Primer name | Nematode | 5′-3′ Sequence | Band size | References |
---|---|---|---|---|
ITS5 | Forward | GGAAGTAAAAGTCGTAACAAGG | – |
|
PITSp4 |
|
ACAACAGCAATCGTCGAG | 265 bp |
|
PITSr3 |
|
AGCGCAGACATGCCGCAA | 434 bp |
Species | Shape of knob | J2 stylet length (µm) | Number of cuticula ridges | Granek’s ratio |
---|---|---|---|---|
|
Rounded | (21.8) | 16-31 (>14) | 1.3-9.5 (>3) |
|
Pointed | 22-24 (23.8) | 8-20 (<14) | 1.2-3.5 (<3) |
Moroccan population |
Pointed | 22.6 | 9 | 2.2 |