Comparison of two in vitro methods for the detection of ivermectin resistance in Haemonchus contortus in sheep
, , y | 22 abr 2016
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Article Category: Research Article
Publicado en línea: 22 abr 2016
Páginas: 120 - 125
Recibido: 10 sept 2014
Aceptado: 10 dic 2015
DOI: https://doi.org/10.1515/helmin-2015-0002
© 2016 Institute of Parasitology, SAS, Košice
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Resistance factors for ivermectin (IVM) in the larval migration inhibition test
| Strains | IVM RF50 | IVM RF99 |
|---|---|---|
| WR/McM | 3.68 | 3.13 |
| CAVR/McM | 2.71 | 7.17 |
| MOX23/McM | 2.06 | 12.27 |
| ISE-SL/McM | 3.54 | 17.20 |
| ISE-SLI/McM | 4.90 | 8.16 |
| WR/ISE | 3.04 | 2.92 |
| CAVR/ISE | 2.24 | 6.70 |
| MOX23/ISE | 1.70 | 11.45 |
| ISE-SL/ISE | 2.92 | 16.06 |
| ISE-SLI/ISE | 4.04 | 7.62 |
Mean reduction (%) in motility of L3 larvae of resistant and susceptible strains of H. contortusafter incubation in different concentrations of eprinomectin (EPM)
| Concentration of EPM (μg/ml) | |||||||
|---|---|---|---|---|---|---|---|
| Isolate | 0.1 | 1 | 10 | 100 | 200 | 300 | LD50 |
| WR | 43.4 | 43 | 53.3 | 72.2 | 71.7 | 41.04 | |
| CAVR | 35 | 49.48 | 29.3 | 65.3 | 64.7 | 49.77 | |
| McM | 0 | 37.1 | 76.4 | 63.9 | 100 | 100 | 3.98 |
| ISE | 34.6 | 53.23 | 65.2 | 69.3 | 100 | 100 | 1.54 |
| MOX23 | 15.8 | 27.7 | 40.3 | 70.7 | 100 | 100 | 15.4 |
| ISE SL | 14.26 | 11.65 | 68.5 | 50.16 | 45.1 | 66.9 | 47.27 |
Resistance factors for ivermectin (IVM) and eprinomectin (EPM) in the micromotility test
| Strains | IVM RF50 | EPM RF50 |
|---|---|---|
| WR/McM | 57.81 | 10.31 |
| CAVR/McM | 2.67 | 12.50 |
| MOX23/McM | 1.00 | 3.87 |
| ISE-SL/McM | 49.89 | 11.88 |
| WR/ISE | 108.05 | 26.65 |
| CAVR/ISE | 5.00 | 32.32 |
| MOX23/ISE | 1.87 | 10.00 |
| ISE-SL/ISE | 93.25 | 30.69 |
Origin and status of anthelmintic susceptibility (S) or resistance (R) of ISE, McMaster, ISE-SL, ISE-SLI, MOX-23, WR and CAVR isolates of Haemonchus contortus
| Isolate | R/S | Reference | Origin |
|---|---|---|---|
| ISE | susceptible | Kenya | |
| McMaster | susceptible | CSIRO, Armidale | Australia |
| ISE-SL | IVM | Great Britain | |
| ISE-SLI | IVM | Great Britain | |
| MOX-23 | IVM | Prichard et al. (2002) | Canada |
| WR | BZ, CLO, IVM, RAF | South Africa | |
| CAVR | IVM, BZ | LeJambre et al. (1995) | Australia |
Arithmetic mean ± SD of LD50 and LD99 for susceptible and resistant isolates obtained in the larval migration inhibition test with ivermectin
| Isolate | LD50(μg/ml) ± SD | LD99(μg/ml) ± SD |
|---|---|---|
| McMaster | 1.03 ±0.72 | 2.80 ±0.61 |
| ISE | 1.25 ±0.48 | 3.00 ±1.24 |
| MOX23 | 2.13 ±1.77 | 34.36 ±25.14 |
| CAVR | 2.80 ± 0.72 | 20.10 ±12.12 |
| WR | 3.80 ± 0.35 | 8.77 ± 4.92 |
| ISE-SL | 3.65 ± 0.48 | 48.18 ±57.59 |
| ISE-SLI | 5.05 ±1.98 | 22.87 ± 7.45 |
Mean reduction (%) in motility of L3 larvae of resistant and susceptible strains of H. contortusafter incubation in different concentrations of ivermectin (IVM)
| Concentration of IVM (μg/ml) | |||||
|---|---|---|---|---|---|
| Isolate | 0.1 | 1 | 10 | 100 | LD50 |
| WR | 0 | 0 | 26.2 | 49.7 | 90.76 |
| CAVR | 0 | 55.2 | 52.8 | 76.1 | 4.2 |
| McMaster | 0 | 39.7 | 88.9 | 80.9 | 1.57 |
| ISE | 0 | 59.3 | 97.8 | 91.4 | 0.84 |
| MOX23 | 13.7 | 41.2 | 78.9 | 96.4 | 1.57 |
| ISE SL | 11.2 | 32 | 6.7 | 80.5 | 78.33 |