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Comparison of two in vitro methods for the detection of ivermectin resistance in Haemonchus contortus in sheep

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Resistance factors for ivermectin (IVM) in the larval migration inhibition test

StrainsIVM RF50IVM RF99
WR/McM3.683.13
CAVR/McM2.717.17
MOX23/McM2.0612.27
ISE-SL/McM3.5417.20
ISE-SLI/McM4.908.16
WR/ISE3.042.92
CAVR/ISE2.246.70
MOX23/ISE1.7011.45
ISE-SL/ISE2.9216.06
ISE-SLI/ISE4.047.62

Mean reduction (%) in motility of L3 larvae of resistant and susceptible strains of H. contortusafter incubation in different concentrations of eprinomectin (EPM)

Concentration of EPM (μg/ml)
Isolate0.1110100200300LD50
WR43.44353.372.271.741.04
CAVR3549.4829.365.364.749.77
McM037.176.463.91001003.98
ISE34.653.2365.269.31001001.54
MOX2315.827.740.370.710010015.4
ISE SL14.2611.6568.550.1645.166.947.27

Resistance factors for ivermectin (IVM) and eprinomectin (EPM) in the micromotility test

StrainsIVM RF50EPM RF50
WR/McM57.8110.31
CAVR/McM2.6712.50
MOX23/McM1.003.87
ISE-SL/McM49.8911.88
WR/ISE108.0526.65
CAVR/ISE5.0032.32
MOX23/ISE1.8710.00
ISE-SL/ISE93.2530.69

Origin and status of anthelmintic susceptibility (S) or resistance (R) of ISE, McMaster, ISE-SL, ISE-SLI, MOX-23, WR and CAVR isolates of Haemonchus contortus

IsolateR/SReferenceOrigin
ISEsusceptibleRoos et al. (2004)Kenya
McMastersusceptibleCSIRO, ArmidaleAustralia
ISE-SLIVMColes (2005)Great Britain
ISE-SLIIVMColes (2005)Great Britain
MOX-23IVMPrichard et al. (2002)Canada
WRBZ, CLO, IVM, RAFVan Wyk and Malan (1988)South Africa
CAVRIVM, BZLeJambre et al. (1995)Australia

Arithmetic mean ± SD of LD50 and LD99 for susceptible and resistant isolates obtained in the larval migration inhibition test with ivermectin

IsolateLD50(μg/ml) ± SDLD99(μg/ml) ± SD
McMaster1.03 ±0.722.80 ±0.61
ISE1.25 ±0.483.00 ±1.24
MOX232.13 ±1.7734.36 ±25.14
CAVR2.80 ± 0.7220.10 ±12.12
WR3.80 ± 0.358.77 ± 4.92
ISE-SL3.65 ± 0.4848.18 ±57.59
ISE-SLI5.05 ±1.9822.87 ± 7.45

Mean reduction (%) in motility of L3 larvae of resistant and susceptible strains of H. contortusafter incubation in different concentrations of ivermectin (IVM)

Concentration of IVM (μg/ml)
Isolate0.1110100LD50
WR0026.249.790.76
CAVR055.252.876.14.2
McMaster039.788.980.91.57
ISE059.397.891.40.84
MOX2313.741.278.996.41.57
ISE SL11.2326.780.578.33
eISSN:
1336-9083
ISSN:
0440-6605
Lingua:
Inglese
Frequenza di pubblicazione:
4 volte all'anno
Argomenti della rivista:
Life Sciences, Zoology, Ecology, other, Medicine, Clinical Medicine, Microbiology, Virology and Infection Epidemiology