<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In order to improve the viable counts of <italic>Bifidobacterium bifidum</italic> BB01 in the liquid medium, the Central Composite Design (CCD) was used to optimize the nitrogen source in the medium of <italic>B. bifidum</italic> BB01. The results showed that the nitrogen source composition of <italic>B. bifidum</italic> BB01 was: peptone 0.9%, yeast extracts 0.3%, beef paste 0.7%. Under the optimal conditions, the viable counts of <italic>B. bifidum</italic> BB01 reached (2.49±0.06)×109CFU/mL after cultured at 18h, which was 42.97% higher than MRS (lactose), and 12.85% higher than the optimized MRS medium (carbon source and prebiotics were optimized). Therefore, the CCD used in this study is workable for promoting the growth of <italic>B. bifidum BB01.</italic></p></abstract>

In order to improve the viable counts of Bifidobacterium bifidum BB01 in the liquid medium, the Central Composite Design (CCD) was used to optimize the nitrogen source in the medium of B. bifidum BB01. The results showed that the nitrogen source composition of B. bifidum BB01 was: peptone 0.9%, yeast extracts 0.3%, beef paste 0.7%. Under the optimal conditions, the viable counts of B. bifidum BB01 reached (2.49±0.06)×109CFU/mL after cultured at 18h, which was 42.97% higher than MRS (lactose), and 12.85% higher than the optimized MRS medium (carbon source and prebiotics were optimized). Therefore, the CCD used in this study is workable for promoting the growth of B. bifidum BB01.

Optimization of nitrogen source for <italic>Bifidobacterium bifidum</italic> using response surface methodology

Shaanxi University of Science & Technology

Acta Universitatis Cibiniensis. Series E: Food Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Optimization of nitrogen source for  using response surface methodology

In order to improve the viable counts of Bifidobacterium bifidum BB01 in the liquid medium, the Central Composite Design (CCD) was used to optimize the...

Optimization of nitrogen source for Bifidobacterium bifidum using response surface methodology

Publicado en línea: 18 jun 2016

Páginas: 53 - 64

DOI: https://doi.org/10.1515/aucft-2016-0004

Palabras clave
response surface methodology, optimization, nitrogen source

© 2016 Lucian Blaga University, published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Optimization of nitrogen source for Bifidobacterium bifidum using response surface methodology

Publicado en línea: 18 jun 2016

Páginas: 53 - 64

DOI: https://doi.org/10.1515/aucft-2016-0004

Palabras claveresponse surface methodology, optimization, nitrogen source

© 2016 Lucian Blaga University, published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
response surface methodology, optimization, nitrogen source