<abstract xmlns="http://www.w3.org/1999/xhtml">

The fig-tree skeletonizer moth, <italic>Choreutis nemorana</italic> (Hübner, 1799) (Lepidoptera, Choreutidae), is a widespread species in the Mediterranean region. In October 2023 and June 2024, during pest control operations as part of the harvesting campaign in the fig groves of the Bordj Ghedir and Wilaya de Bouira regions (Algeria), <italic>C. nemorana</italic> was documented for the first time in Algeria.
</abstract>

Laboratory of Characterization and Valorisation of Natural Resources (LCVRN)

Mohamed El Bachir El Ibrahimi de Bordj Bou Arréridj

(CREA) Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia Agraria - Centro di Ricerca Olivicoltura

Laboratoire Biotechnologies et Protection des Ecosystèmes Agricoles et Naturels

Faculté des Sciences de la Nature et Sciences de la Terre, Université de Bouira

Laboratory of Biological Oceanography and the Marine Environment (LOBEM)

Faculty of Biological Sciences, University of Science and Technology of Houari Boumediene (USTHB)

The fig-tree skeletonizer moth, <italic>Choreutis nemorana</italic> (Hübner, 1799) (Lepidoptera: Choreutidae), a new species for the Algeria fauna

<abstract xmlns="http://www.w3.org/1999/xhtml">

The current study evaluated the effect of microbial-derived insecticides (abamectin and spinosad), two microbials (<italic>Beauveria bassiana</italic> (Balsamo) Vuillemin, <italic>Bacillus thuringiensis</italic> Berliner), and three chitin synthesis inhibitors (CSIs) (chlorfluazuron, hexaflumuron and lufenuron) against <italic>Spodoptera frugiperda</italic> larvae. Spinosad and abamectin caused pronounced mortality against second larval instar of <italic>S. frugiperda</italic> using the leaf dipping method. Spinosad induced higher toxicity (LC50 = 4.01 mg/L) than abamectin (LC50 = 8.33 mg/L) one day after treatment. The treatments with <italic>B. bassiana</italic> and <italic>B. thuringiensis</italic> caused higher mortality of <italic>S. frugiperda</italic> larvae 7 days after treatment with LC50 values of 3.0 × 105 spores/ml and 8.2 × 106 cells/ml, respectively. In the case of the CSIs, hexaflumuron showed higher toxicity than chlorfluazuron and lufenuron with LC50 values of 0.01, 0.009 and 0.005 mg/L 3, 7 and 10 days after treatment, respectively.
</abstract>

Faculty of Agriculture, Al-Azhar University

Department of Pesticide Chemistry and Technology

Faculty of Agriculture, 21545-El-Shatby, Alexandria University

Comparative effect of biopesticides against the fall armyworm <italic>Spodoptera frugiperda</italic> (J.E. Smith)

<abstract xmlns="http://www.w3.org/1999/xhtml">

Five fungal endophytes, <italic>Alternaria</italic> sp., <italic>Aspergillus</italic> sp., <italic>Chaetomium</italic> sp., <italic>Rhizopus</italic> sp. and <italic>Curvularia</italic> sp., were isolated from an Egyptian herbaceous plant, <italic>Tribulus terrestris</italic>, and tested for their antibacterial activity against three phytopathogenic bacteria (<italic>Pectobacterium carotovorum</italic> subsp. <italic>carotovorum, Ralstonia solanacearum, Pseudomonas syringae</italic> pv. <italic>syringae</italic>). <italic>Chaetomium</italic> sp. showed the highest antibacterial activity. This strain was identified morphologically and molecularly as <italic>Chaetomium cochliodes</italic> MS03 (MW898133) based on the ITS1-5.8S rRNA-ITS2 genomic region. <italic>Chaetomium cochliodes</italic> caused 15 and 8 mm inhibition zones of <italic>P. carotovorum</italic> subsp. <italic>carotovorum</italic> and <italic>R. solanacearum</italic>, respectively. <italic>Chaetomium cochliodes</italic> isolate was fermented and extracted with ethyl acetate. The crude extract of <italic>C. cochliodes</italic> showed strong antibacterial activity against <italic>P. carotovorum</italic> subsp. <italic>carotovorum</italic> (inhibition zone = 27 mm). Bioassay guided isolation of the crude extract using silica gel column chromatography was conducted to isolate bioactive secondary metabolites. Minimum inhibitory concentrations (MICs) were 500, 32 and 4 mg/L for <italic>C. cochliodes</italic> extract, fraction 14 and fraction 15, respectively, against <italic>P. carotovorum</italic> subsp. <italic>carotovorum</italic>. Bioactive fractions were analyzed by GC/MS. The bioactive pure compound was identified as 9,12-octadecadienoic acid (Z,Z) and the chemical structure was confirmed by H1 NMR and C13 NMR spectral analysis. The isolated compound showed a promising antibacterial activity against <italic>P. carotovorum</italic> subsp. <italic>carotovorum</italic> with MIC value of 32 mg/L.
</abstract>

Antibacterial potential of extracts and metabolites isolated from the endophytic fungus <italic>Chaetomium cochliodes</italic> against phytopathogenic bacteria

<abstract xmlns="http://www.w3.org/1999/xhtml">

Corn weevil (<italic>Sitophilus zeamais)</italic> is one of the most destructive pests of corn seeds during storage. The weevil may be a vector of mycotoxigenic fungi or yeast contaminating seed lots. In this study, an unknown yeast species was isolated from corn weevils found in stored corn seeds. We hypothesized that this yeast had an antifungal activity thereby inhibiting growth of mycotoxigenic fungi in corn seeds. The yeast species was identified as <italic>Hyphopichia burtonii</italic>, using combined morphological and molecular assays, and its potential inhibitory activity was assessed <italic>in vitro</italic> (spread plate and dual culture) against three known mycotoxigenic fungi, <italic>Fusarium verticillioides, Aspergillus niger</italic> and <italic>A. fl avus</italic>. Screening of the antagonistic activity of the yeast isolate showed 50 – 69% colony growth inhibition of three fungi when the yeast was spread plated on PDA but only slight inhibition (5.8 – 13.7% growth inhibition) in the dual culture assay. The sporulation of the fungi was also affected at 57 – 96% and 29 – 40% in spread plating and dual culture assay, respectively. In addition, volatile and non-volatile fractions also showed a reduction in mycelial growth. Variable responses were observed among the mycotoxigenic fungi. Further research would be interesting on the potential utilization of the antagonistic yeast to reduce fungal growth and sporulation, and possible mitigation of mycotoxin contamination in corn grains. To our knowledge, this is the first record of <italic>H. burtonii</italic> isolated from an insect, specifically <italic>S. zeamais.</italic>
</abstract>

Department of Science and Technology-Science Education Institute

Department of Biology, College of Science

First record of <italic>Hyphopichia burtonii</italic> isolated from the storage pest <italic>Sitophilus zeamais</italic> and its bioactivity against mycotoxigenic fungi

<abstract xmlns="http://www.w3.org/1999/xhtml">

The spotted wing drosophila, <italic>Drosophila suzukii</italic> (Matsumura, 1931), population fluctuations and distribution were monitored in four susceptible crops (blueberry, strawberry, cherry, wine grapes) and five major agricultural regions of Georgia (Guria, Samegrelo, Imereti, Kartli and Kakheti) during the years 2021 and 2022 with the aim to study the population size and evaluate the pest status. Population monitoring was conducted in four locations of each studied region on a weekly basis from May to November using PHEROCON® SWD traps with PHEROCON® SWD PEEL-PAK™ Broad Spectrum Lures. The investigation indicated significant growth of population from 2021 to 2022 in most of the studied locations. Population increase was detected in all crop orchards except cherries. Τhe absence of alternative host plants at crop proximity was of critical importance to save the crop from pest invasion. The crop plant species did not have a significant impact on <italic>D. suzukii</italic>. Pest population was significantly larger in summer and autumn compared to spring, possibly influenced by the ripening of alternative crops such as blackberry and elderberry. The sex ratio between male and female individuals was almost 1:1 and remained consistent through the two-year study period and across regions. We consider that <italic>D. suzukii</italic> entered the country from the southwestern part and extended its distribution range towards the east. No strategies for <italic>D. suzukii</italic> control have been elaborated in Georgia so far. Sprays of effective pesticides based on pest monitoring as well as sanitation measures involving removal of alternative host plants and any crop residues from the field are necessary to avoid pest outbreak.
</abstract>

I. Javakhishvili Tbilisi State University

CNFA’s representative office in Georgia

Population monitoring and status evaluation of the new invasive pest, <italic>Drosophila suzukii</italic> (Matsumura) (Diptera: Drosophilidae), in various crop systems of Georgia (Sakartvelo)

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Hellenic Plant Protection Journal

 The Hellenic Plant Protection Journal (HPPJ) (ISSN 1791-3691) is the scientific publication of the Benaki Phytopathological Institute (BPI) replacing the Annals of the Benaki Phytopathological Institute (ISSN 1790-1480) which had been published since 1935. Starting from January 2008, the Hellenic Plant Protection Journal is published semiannually, in January and July each year.  HPPJ publishes scientific work on all aspects of plant health and plant protection referring to plant pathogens, pests, weeds, pesticides and relevant environmental and safety issues. In addition, the topics of the journal extend to aspects related to pests of public health in agricultural and urban areas.  Papers submitted for publication can be either in the form of a complete research article or in the form of a sufficiently documented short communication (including new records). Only original articles which have not been published or submitted for publication elsewhere are considered for publication in the journal. Review articles in related topics, either submitted or invited by the Editorial Board, are also published, normally one article per issue. Upon publication all articles are copyrighted by the BPI.  Manuscripts should be prepared according to instructions available to authors and submitted in electronic form through Editorial Manager. All submitted manuscripts are considered and published after successful completion of a review procedure by two competent referees.  The content of the articles published in HPPJ reflects the view and the official position of the authors. The information and opinions contained herein have not been adopted or approved by the HPPJ Editorial Board. The HPPJ Editorial Board neither guarantees the accuracy of the information included in the published articles nor may be held responsible for the use to which information contained herein may be put.  For all parties involved in the act of publishing (the author(s), the journal editor(s), the peer reviewers, and the publisher) it is necessary to agree upon standards of expected ethical behavior. HPPJ follows the ethics statements of De Gruyter journals, which are based on the Committee on Publication Ethics (COPE) Code of Conduct guidelines available at www.publicationethics.org.  Archiving  Sciendo archives the contents of this journal in Portico - digital long-term preservation service of scholarly books, journals and collections.  Plagiarism Policy  The editorial board is participating in a growing community of Similarity Check System's users in order to ensure that the content published is original and trustworthy. Similarity Check is a medium that allows for comprehensive manuscripts screening, aimed to eliminate plagiarism and provide a high standard and quality peer-review process. 

Hellenic Plant Protection Journal

Volume 17 (2024): Issue 2 (July 2024)

The fig-tree skeletonizer moth, Choreutis nemorana (Hübner, 1799) (Lepidoptera: Choreutidae), a new species for the Algeria fauna

Comparative effect of biopesticides against the fall armyworm Spodoptera frugiperda (J.E. Smith)

Antibacterial potential of extracts and metabolites isolated from the endophytic fungus Chaetomium cochliodes against phytopathogenic bacteria

First record of Hyphopichia burtonii isolated from the storage pest Sitophilus zeamais and its bioactivity against mycotoxigenic fungi

Population monitoring and status evaluation of the new invasive pest, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), in various crop systems of Georgia (Sakartvelo)

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