<abstract xmlns="http://www.w3.org/1999/xhtml">

Fungi belonging to the Botryosphaeriaceae family are widespread pathogens of many angiosperms, causing disease on various high value crops. The most important members of the family for the Greek region and other Mediterranean countries are <italic>Botryosphaeria dothidea</italic>, <italic>Neofusicoccum hellenicum</italic>, <italic>Neofusicoccum mediterraneum</italic> and <italic>Neofusicoccum parvum</italic>. The frequently concurrent isolation of Botryosphaeriaceae species from the same host, as well as the extensive host range of <italic>B. dothidea</italic>, necessitate the development of rapid and reliable detection methods. This study presents a new and robust molecular diagnostic tool, in the form of a PCR method based on primers designed on an SNP (single nucleotide polymorphism) located in the ITS region (Internal Transcribed Region) of <italic>B. dothidea</italic> and <italic>Neofusicoccum</italic> species. SNP primers constructed with or without added mismatch nucleotides were combined with the same upstream universal primer to generate distinct amplicons. When evaluated in PCR assays, mismatched primers were found to have the highest differentiation capability. The potential for further development of SNP assays in order to differentiate between species is being evaluated.
</abstract>

Differentiation between <italic>Botryosphaeria dothidea</italic> and <italic>Neofusicoccum</italic> spp. based on a single nucleotide polymorphism in the ITS region

<abstract xmlns="http://www.w3.org/1999/xhtml">

Efficiency of various organic acids, organic salts, essential oils, algae (an extract of <italic>Chlorella vulgaris</italic> and a commercial product), and bioagents against <italic>Magnaporthiopsis maydis</italic>, causing maize late wilt disease, was evaluated in laboratory and field conditions. For the <italic>in vitro</italic> tests, isolated <italic>M. maydis</italic> field strains from Egypt were used. Additionally, in field experiments different application methods were tested for their efficacy throughout two successive growing seasons. Results showed maximum growth inhibition of <italic>M. maydis</italic> at different concentrations of salicylic acid, ascorbic acid, benzoic acid and humic acid as well as sodium benzoate, potassium sorbate, di-potassim phosphate and calcium choloride, in descending order. The essential oils of carnation, lemongrass and black seed followed a similar trend. The minimum pathogenic fungal growth was achieved when the pathogen was exposed to the antagonistic <italic>Trichoderma viride</italic> followed by <italic>Trichoderma harzianum, Bacillus subtilis</italic> and <italic>Pseudomonas fluorescens</italic> treatments. Under field conditions, the highest disease reduction was recorded after treatment with salicylic acid (all application methods), humic acid (all application methods), sodium benzoate (seed dressing), carnation oil (seed dressing or seed dressing +foliar spray) and the bioagents <italic>B. subtilis</italic> and <italic>P. fluorescens</italic> (soil drench). Overall, all treatments and all application methods led to significant lower disease incidence compared to the untreated control in both growing seasons. Additionally, all treatments achieved an enhancement of plant morpho-genesis and yield characters. The most effective combinations of compounds/bioagents and application methods can be considered for future use in IPM management of late wilt disease of maize.
</abstract>

Efficacy of fungicide alternatives against late wilt disease of maize and their influence on plant morphogenesis and yield characters

<abstract xmlns="http://www.w3.org/1999/xhtml">

The Nearctic weevil <italic>Sphenophorus placidus vestitus</italic> Chittenden, 1904, an alien pest which is known to infest turfgrasses is reported for the first time on the island of Cyprus. A single specimen was collected from Famagusta (Ammochostos), Protaras in 2012. The species distribution and possible economic impacts are shortly discussed. A checklist for the alien Dryophthorinae of Cyprus is presented.
</abstract>

Department of Forests, Ministry of Agriculture, Rural Development and Environment

Section of Ecology and Systematics, Department of Biology

National and Kapodistrian University of Athens

First record of <italic>Sphenophorus placidus vestitus</italic> (Coleoptera: Curculionidae: Dryophthorinae) in Cyprus

<abstract xmlns="http://www.w3.org/1999/xhtml">

The Nearctic spider beetle <italic>Ozognathus cornutus</italic> (LeConte, 1859) is recorded for the first time in Cyprus during entomological surveys on alien <italic>Eucalyptus</italic> spp. The biology of this alien species is still understudied. A short overview of the species distribution and ecology in the Mediterranean is presented, adding a new host plant for the beetle.
</abstract>

Department of Ecology and Systematics, Faculty of Biology

First record of the Nearctic <italic>Ozognathus cornutus</italic> (LeConte, 1859) (Coleoptera: Ptinidae: Anobiinae) in Cyprus

<abstract xmlns="http://www.w3.org/1999/xhtml">

Biological parameters of phylloxera local strains as well as infestation were studied on Syrian grapevine varieties including Baladi, Salamone, Karawane, Hafarzale, Gharbe and the American resistance rootstock (ARR-B41) (<italic>Vitis vinifera</italic> x <italic>Vitis berlandieri</italic>). Root artificial infestation method revealed significant differences in phylloxera population between the majority of tested varieties comparing to the ARR-B41 rootstock. Hafarzale and Karawane have shown the lowest population of grape phylloxera, with a similar level of resistance to rootstock ARR-B41. Furthermore, Salamone and Gharbe are more conducive to grape phylloxera reproduction. Field investigation has shown significant differences between the local varieties and ARR-B41 in terms of the average number of galls “nodosities”. Overall, Hafarzale and Karawane tended to form significantly fewer galls than the other varieties.
</abstract>

Biological parameters of grape phylloxera, <italic>Daktulosphaira vitifoliae</italic>, on local grapevine varieties in central Syria – Implications on their susceptibility

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Hellenic Plant Protection Journal

 The Hellenic Plant Protection Journal (HPPJ) (ISSN 1791-3691) is the scientific publication of the Benaki Phytopathological Institute (BPI) replacing the Annals of the Benaki Phytopathological Institute (ISSN 1790-1480) which had been published since 1935. Starting from January 2008, the Hellenic Plant Protection Journal is published semiannually, in January and July each year.  HPPJ publishes scientific work on all aspects of plant health and plant protection referring to plant pathogens, pests, weeds, pesticides and relevant environmental and safety issues. In addition, the topics of the journal extend to aspects related to pests of public health in agricultural and urban areas.  Papers submitted for publication can be either in the form of a complete research article or in the form of a sufficiently documented short communication (including new records). Only original articles which have not been published or submitted for publication elsewhere are considered for publication in the journal. Review articles in related topics, either submitted or invited by the Editorial Board, are also published, normally one article per issue. Upon publication all articles are copyrighted by the BPI.  Manuscripts should be prepared according to instructions available to authors and submitted in electronic form through Editorial Manager. All submitted manuscripts are considered and published after successful completion of a review procedure by two competent referees.  The content of the articles published in HPPJ reflects the view and the official position of the authors. The information and opinions contained herein have not been adopted or approved by the HPPJ Editorial Board. The HPPJ Editorial Board neither guarantees the accuracy of the information included in the published articles nor may be held responsible for the use to which information contained herein may be put.  For all parties involved in the act of publishing (the author(s), the journal editor(s), the peer reviewers, and the publisher) it is necessary to agree upon standards of expected ethical behavior. HPPJ follows the ethics statements of De Gruyter journals, which are based on the Committee on Publication Ethics (COPE) Code of Conduct guidelines available at www.publicationethics.org.  Archiving  Sciendo archives the contents of this journal in Portico - digital long-term preservation service of scholarly books, journals and collections.  Plagiarism Policy  The editorial board is participating in a growing community of Similarity Check System's users in order to ensure that the content published is original and trustworthy. Similarity Check is a medium that allows for comprehensive manuscripts screening, aimed to eliminate plagiarism and provide a high standard and quality peer-review process. 

Hellenic Plant Protection Journal

Volume 15 (2022): Issue 2 (July 2022)

Differentiation between Botryosphaeria dothidea and Neofusicoccum spp. based on a single nucleotide polymorphism in the ITS region

Efficacy of fungicide alternatives against late wilt disease of maize and their influence on plant morphogenesis and yield characters

First record of Sphenophorus placidus vestitus (Coleoptera: Curculionidae: Dryophthorinae) in Cyprus

First record of the Nearctic Ozognathus cornutus (LeConte, 1859) (Coleoptera: Ptinidae: Anobiinae) in Cyprus

Biological parameters of grape phylloxera, Daktulosphaira vitifoliae, on local grapevine varieties in central Syria – Implications on their susceptibility

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