<abstract xmlns="http://www.w3.org/1999/xhtml">The present study is focused on the determination of optimized activity conditions of peroxidase (POD) of turnip (<italic>Brassica rapa</italic>). The enzyme is extracted from turnip with 0.5M Na2HPO4 (pH7.0). Peroxidase activity is evaluated by monitoring the oxidation of guaiacol to tetraguaiacol in the presence of hydrogen peroxide (H2O2). Before the optimization of peroxidase activity from a turnip by response surface methodology (RSM), a preliminary step is conducted for each studied parameter (pH, temperature, and NaCl concentration). Further, RSM following Box-Behnken design is used in order to refine the optimization of the conditions. Response surface analysis showed that parameter values with the highest peroxidase activity are 5.9, 29.8°C, and 1.07M for pH, temperature, and NaCl, respectively. Under these experimental conditions, peroxidase activity is found to be 4027.25U/mg. The obtained experimental value agrees with that of the calculated one, indicating the pertinence of the developed model for the optimization of peroxidase activity.</abstract>

The present study is focused on the determination of optimized activity conditions of peroxidase (POD) of turnip (Brassica rapa). The enzyme is extracted from turnip with 0.5M Na2HPO4 (pH7.0). Peroxidase activity is evaluated by monitoring the oxidation of guaiacol to tetraguaiacol in the presence of hydrogen peroxide (H2O2). Before the optimization of peroxidase activity from a turnip by response surface methodology (RSM), a preliminary step is conducted for each studied parameter (pH, temperature, and NaCl concentration). Further, RSM following Box-Behnken design is used in order to refine the optimization of the conditions. Response surface analysis showed that parameter values with the highest peroxidase activity are 5.9, 29.8°C, and 1.07M for pH, temperature, and NaCl, respectively. Under these experimental conditions, peroxidase activity is found to be 4027.25U/mg. The obtained experimental value agrees with that of the calculated one, indicating the pertinence of the developed model for the optimization of peroxidase activity.

Optimization of Peroxidase Activity of Turnip (<italic>Brassica rapa</italic>) Using Response Surface Methodology

Laboratoire de Biochimie Appliquée, Département des Sciences Alimentaires, Faculté des Sciences de la Nature et de la Vie

Acta Universitatis Cibiniensis. Series E: Food Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Optimization of Peroxidase Activity of Turnip () Using Response Surface Methodology

The present study is focused on the determination of optimized activity conditions of peroxidase (POD) of turnip (Brassica rapa). The enzyme is extracted from...

Optimization of Peroxidase Activity of Turnip (Brassica rapa) Using Response Surface Methodology

Online veröffentlicht: 24. Dez. 2020

Seitenbereich: 186 - 194

Eingereicht: 24. Juli 2020

Akzeptiert: 12. Okt. 2020

DOI: https://doi.org/10.2478/aucft-2020-0017

Schlüsselwörter
Peroxidase activity, turnip, optimization, response surface methodology

© 2020 Amel Dahdouh et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Optimization of Peroxidase Activity of Turnip (Brassica rapa) Using Response Surface Methodology

Online veröffentlicht: 24. Dez. 2020

Seitenbereich: 186 - 194

Eingereicht: 24. Juli 2020

Akzeptiert: 12. Okt. 2020

DOI: https://doi.org/10.2478/aucft-2020-0017

SchlüsselwörterPeroxidase activity, turnip, optimization, response surface methodology

© 2020 Amel Dahdouh et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Schlüsselwörter
Peroxidase activity, turnip, optimization, response surface methodology