<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>This study aimed to evaluate the methane-reducing potential of individual and combined treatments of low levels of nitrate (NIT) and a mixture of capric/lauric acid (CL) in dairy cows. Both <italic>in vitro</italic> and <italic>in vivo</italic> experiments were conducted. In the <italic>in vitro</italic> experiment, the anti-methanogenic effects of NIT (1.825 mmol/l) and CL (250 mg/l; capric acid, 125 mg/l + lauric acid, 125 mg/l) were evaluated in a 2 × 2 factorial design using consecutive batch incubations with rumen fluid. The NIT and CL reduced (P&lt;0.05) methane production by 9.2% and by 21.3%, respectively. However, combining NIT with CL did not show (P>0.05) any benefit in methane reduction compared to the use of CL alone. In the <italic>in vivo</italic> experiment, eight multiparous dry Holstein cows were fed two diets in a crossover design for two 21-day periods (14 days of adaptation and 7 days of sampling). The treatments were: 1) silage-based basal diet + 100 g stearic acid per cow/d (CON) and 2) silage-based basal diet + 50 g capric acid + 50 g lauric acid per cow/d (CL). Gas emissions were measured using open-circuit respiration chambers. Methane production (g/d) was reduced (by 11.5%; P = 0.012) when the diet was supplemented with CL. However, supplementation with CL increased ruminal ammonia-N concentration (by 28.5%; P = 0.015) and gas ammonia production (g/d; by 37.2%; P = 0.005). Ruminal pH, protozoa count, and total and individual volatile fatty acid concentrations (VFA ) did not differ (P>0.05) between the treatments. Treatment did not affect the intake and apparent total tract digestibility (P>0.05). In conclusion, our results suggest that low CL levels have anti-methanogenic potential. However, low levels of CL may compromise nitrogen use efficiency.</p>
</abstract>

This study aimed to evaluate the methane-reducing potential of individual and combined treatments of low levels of nitrate (NIT) and a mixture of capric/lauric acid (CL) in dairy cows. Both in vitro and in vivo experiments were conducted. In the in vitro experiment, the anti-methanogenic effects of NIT (1.825 mmol/l) and CL (250 mg/l; capric acid, 125 mg/l + lauric acid, 125 mg/l) were evaluated in a 2 × 2 factorial design using consecutive batch incubations with rumen fluid. The NIT and CL reduced (P<0.05) methane production by 9.2% and by 21.3%, respectively. However, combining NIT with CL did not show (P>0.05) any benefit in methane reduction compared to the use of CL alone. In the in vivo experiment, eight multiparous dry Holstein cows were fed two diets in a crossover design for two 21-day periods (14 days of adaptation and 7 days of sampling). The treatments were: 1) silage-based basal diet + 100 g stearic acid per cow/d (CON) and 2) silage-based basal diet + 50 g capric acid + 50 g lauric acid per cow/d (CL). Gas emissions were measured using open-circuit respiration chambers. Methane production (g/d) was reduced (by 11.5%; P = 0.012) when the diet was supplemented with CL. However, supplementation with CL increased ruminal ammonia-N concentration (by 28.5%; P = 0.015) and gas ammonia production (g/d; by 37.2%; P = 0.005). Ruminal pH, protozoa count, and total and individual volatile fatty acid concentrations (VFA ) did not differ (P>0.05) between the treatments. Treatment did not affect the intake and apparent total tract digestibility (P>0.05). In conclusion, our results suggest that low CL levels have anti-methanogenic potential. However, low levels of CL may compromise nitrogen use efficiency.

Capric and lauric acid mixture decreased rumen methane production, while combination with nitrate had no further benefit in methane reduction

Department of Nutrition and Feeding of Farm Animals

Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

This study aimed to evaluate the methane-reducing potential of individual and combined treatments of low levels of nitrate (NIT) and a mixture of capric/lauric acid (CL) in dairy cows. Both in vitro and in vivo experiments were conducted. In the in vitro experiment, the anti-methanogenic effects of NIT (1.825 mmol/l) and CL (250 mg/l; capric acid, 125 mg/l + lauric acid, 125 mg/l) were evaluated in a 2 × 2 factorial design using consecutive batch incubations with rumen fluid. The NIT and CL reduced (P0.05) any benefit in methane reduction compared to the use of CL alone. In the in vivo experiment, eight multiparous dry Holstein cows were fed two diets in a crossover design for two 21-day periods (14 days of adaptation and 7 days of sampling). The treatments were: 1) silage-based basal diet + 100 g stearic acid per cow/d (CON) and 2) silage-based basal diet + 50 g capric acid + 50 g lauric acid per cow/d (CL). Gas emissions were measured using open-circuit respiration chambers. Methane production (g/d) was reduced (by 11.5%; P = 0.012) when the diet was supplemented with CL. However, supplementation with CL increased ruminal ammonia-N concentration (by 28.5%; P = 0.015) and gas ammonia production (g/d; by 37.2%; P = 0.005). Ruminal pH, protozoa count, and total and individual volatile fatty acid concentrations (VFA ) did not differ (P>0.05) between the treatments. Treatment did not affect the intake and apparent total tract digestibility (P>0.05). In conclusion, our results suggest that low CL levels have anti-methanogenic potential. However, low levels of CL may compromise nitrogen use efficiency.

This study aimed to evaluate the methane-reducing potential of individual and combined treatments of low levels of nitrate (NIT) and a mixture of capric/lauric...

Capric and lauric acid mixture decreased rumen methane production, while combination with nitrate had no further benefit in methane reduction

Online veröffentlicht: 26. Juli 2023

Seitenbereich: 799 - 808

Eingereicht: 14. Sept. 2022

Akzeptiert: 17. Jan. 2023

DOI: https://doi.org/10.2478/aoas-2023-0010

Schlüsselwörter
rumen fermentation, methane, medium-chain fatty acid, nitrate

© 2023 Miroslav Joch et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Capric and lauric acid mixture decreased rumen methane production, while combination with nitrate had no further benefit in methane reduction

Online veröffentlicht: 26. Juli 2023

Seitenbereich: 799 - 808

Eingereicht: 14. Sept. 2022

Akzeptiert: 17. Jan. 2023

DOI: https://doi.org/10.2478/aoas-2023-0010

Schlüsselwörterrumen fermentation, methane, medium-chain fatty acid, nitrate

© 2023 Miroslav Joch et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Schlüsselwörter
rumen fermentation, methane, medium-chain fatty acid, nitrate