<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in <italic>Enterococcus</italic> spp. isolated from different cheeses and also investigate some of their virulence factors. <italic>Enterococcus</italic> strains were isolated from 33 different cheeses. <italic>Enterococcus faecium</italic> (6 strains) and <italic>Enterococcus faecalis</italic> (5 strains) enterocin-producing strains were identified by 16S rDNA analyses. Structural genes <italic>ent</italic>A, <italic>ent</italic>B, <italic>ent</italic>P and <italic>ent</italic>X were detected in some isolates. Multiple enterocin structural genes were found in 7 strains. None of the tested enterococci demonstrated anyβ-haemolytic activity and only one strain had gelatinase activity. Six strains showed multiple antibiotic resistance patterns and in addition, <italic>vanA</italic> and several virulence genes were detected in many strains. Only <italic>E. faecalis</italic> MBE1-9 showed tyrosine decarboxylase activity and <italic>tdc</italic> gene was detected only in this strain.</p>
</abstract>

The purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in Enterococcus spp. isolated from different cheeses and also investigate some of their virulence factors. Enterococcus strains were isolated from 33 different cheeses. Enterococcus faecium (6 strains) and Enterococcus faecalis (5 strains) enterocin-producing strains were identified by 16S rDNA analyses. Structural genes entA, entB, entP and entX were detected in some isolates. Multiple enterocin structural genes were found in 7 strains. None of the tested enterococci demonstrated anyβ-haemolytic activity and only one strain had gelatinase activity. Six strains showed multiple antibiotic resistance patterns and in addition, vanA and several virulence genes were detected in many strains. Only E. faecalis MBE1-9 showed tyrosine decarboxylase activity and tdc gene was detected only in this strain.

Safety Evaluation of Enterocin Producer <italic>Enterococcus</italic> sp. Strains Isolated from Traditional Turkish Cheeses

Faculty of Engineering, Department of Food Engineering

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Safety Evaluation of Enterocin Producer  sp. Strains Isolated from Traditional Turkish Cheeses

The purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in Enterococcus spp. isolated from...

Safety Evaluation of Enterocin Producer Enterococcus sp. Strains Isolated from Traditional Turkish Cheeses

Published Online: Jun 28, 2017

Page range: 223 - 233

Received: Jul 26, 2016

Accepted: Jan 12, 2017

DOI: https://doi.org/10.5604/pjm-2017-223

© 2017 MINE AVCI et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.