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<p>In the current study, 65 <italic>Vibrio</italic> spp. were isolated from the Monastir lagoon water, were characterized phenotypically and genotypically. In addition, we looked for the presence of three <italic>Vibrio parahaemolyticus</italic> virulence genes (<italic>tlh</italic>, <italic>trh</italic> and <italic>tdh</italic>) and ten <italic>Vibrio cholerae</italic> virulence genes (<italic>ctx</italic>A, <italic>vpi</italic>, <italic>zot</italic>, <italic>ace</italic>, <italic>tox</italic>R, <italic>tox</italic>T, <italic>tos</italic>S, <italic>tox</italic>RS, <italic>tcp</italic>A and <italic>cp</italic>P). We also investigated the antibiotic susceptibilities and the adherence ability of the identified strains to abiotic material and to biotic surfaces. The cytotoxicity activity against HeLa and Vero cell lines were also carried out for all tested strains. All <italic>Vibrio</italic> isolates were identified to the species level and produced several hydrolytic exoenzymes. The results also revealed that all strains were expressing high rates of resistance to tested antibiotics. The minimum inhibitory concentration (MIC) values showed that tetracycline and chloramphenicol were the most effective antibiotics against the tested bacteria. <italic>Vibrio alginolyticus</italic> and <italic>V. cholerae</italic> species were the most adhesive strains to both biotic and abiotic surfaces. Besides, <italic>V. alginolyticus</italic> isolates has the high levels of recombination of genes encoding <italic>V. cholerae</italic> and <italic>V. parahaemolyticus</italic> virulence factors. <italic>In vitro</italic> cytotoxic activities of several <italic>Vibrio</italic> extracellular product were also observed among HeLa and Vero cells.</p>
</abstract>

In the current study, 65 Vibrio spp. were isolated from the Monastir lagoon water, were characterized phenotypically and genotypically. In addition, we looked for the presence of three Vibrio parahaemolyticus virulence genes (tlh, trh and tdh) and ten Vibrio cholerae virulence genes (ctxA, vpi, zot, ace, toxR, toxT, tosS, toxRS, tcpA and cpP). We also investigated the antibiotic susceptibilities and the adherence ability of the identified strains to abiotic material and to biotic surfaces. The cytotoxicity activity against HeLa and Vero cell lines were also carried out for all tested strains. All Vibrio isolates were identified to the species level and produced several hydrolytic exoenzymes. The results also revealed that all strains were expressing high rates of resistance to tested antibiotics. The minimum inhibitory concentration (MIC) values showed that tetracycline and chloramphenicol were the most effective antibiotics against the tested bacteria. Vibrio alginolyticus and V. cholerae species were the most adhesive strains to both biotic and abiotic surfaces. Besides, V. alginolyticus isolates has the high levels of recombination of genes encoding V. cholerae and V. parahaemolyticus virulence factors. In vitro cytotoxic activities of several Vibrio extracellular product were also observed among HeLa and Vero cells.

Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among <italic>Vibrio</italic> spp. Strains Isolated from the Monastir Lagoon, Tunisia

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among  spp. Strains Isolated from the Monastir Lagoon, Tunisia

In the current study, 65 Vibrio spp. were isolated from the Monastir lagoon water, were characterized phenotypically and genotypically. In addition, we looked...

Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among Vibrio spp. Strains Isolated from the Monastir Lagoon, Tunisia

Published Online: Aug 26, 2016

Page range: 307 - 318

Received: Oct 15, 2014

Accepted: Feb 11, 2016

DOI: https://doi.org/10.5604/17331331.1215610

Keywords
spp., antibiotic susceptibility, biofilm, Monastir lagoon, virulence genes

© 2016 BADREDDINE MECHRI et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among Vibrio spp. Strains Isolated from the Monastir Lagoon, Tunisia

Published Online: Aug 26, 2016

Page range: 307 - 318

Received: Oct 15, 2014

Accepted: Feb 11, 2016

DOI: https://doi.org/10.5604/17331331.1215610

Keywordsspp., antibiotic susceptibility, biofilm, Monastir lagoon, virulence genes

© 2016 BADREDDINE MECHRI et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
spp., antibiotic susceptibility, biofilm, Monastir lagoon, virulence genes