Characterization of Serotypes and Molecular Drug Resistance Patterns of Haemophilus influenzae in Kunming Children
, , , , , , , , and | Jun 14, 2023
About this article
Article Category: ORIGINAL PAPER
Published Online: Jun 14, 2023
Page range: 125 - 131
Received: Dec 11, 2022
Accepted: Jan 31, 2023
DOI: https://doi.org/10.33073/pjm-2023-006
Keywords
© 2023 Mei Yuan et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Fig. 1.
Fig. 2.
The primers for drug resistance genes of Haemophilus influenzae (Søndergaard and Nørskov-Lauritsen 2016; Li et al. 2020).
| Gene | Primer | Product size (bp) |
|---|---|---|
| F: TAAGAGAATTATGCAGTGCTGCC |
458 | |
| F: AAGCCCGCATTGGCGTTTAT |
521 | |
| F: GATACTACGTCCTTTAAATTAAG |
551 | |
| F: TTCAAGTAACCGTGGTGTGAC |
465 |
Drug resistance rates of Haemophilus influenzae strains [n (%)].
| Antibiotic | β-lactamase-positive (n = 474) | β-lactamase-negative (n = 326) | χ2 | |
|---|---|---|---|---|
| Ampicillin | 474 (100.0) | 113 (34.6) | 45.725 | ≤ 0.001 |
| Sulfamethoxazole/trimethoprim | 399 (84.2) | 232 (71.2) | 14.558 | ≤ 0.001 |
| Cefaclor | 266 (56.1) | 137 (42.0) | 2.515 | 0.113 |
| Cefuroxime | 225 (47.5) | 110 (33.8) | 0.871 | 0.351 |
| Amoxicillin/clavulanate | 123 (25.9) | 109 (33.4) | 31.911 | ≤ 0.001 |
| Tetracycline | 48 (10.1) | 12 (3.7) | 3.266 | 0.071 |
| Chloramphenicol | 41 (8.6) | 4 (1.2) | 10.184 | 0.001 |
| Cefotaxime | 4 (0.8) | 5 (1.5) | 1.593 | 0.207 |
| Ofloxacin | 2 (0.4) | 1 (0.3) | 0.010 | 0.919 |
| Multidrug resistance | 295 (62.2) | 106 (32.5) | 3.911 | 0.048 |
The primers for capsular genes of Haemophilus influenzae (Fella et al. 1994).
| Gene | Primer | Product size (bp) |
|---|---|---|
| F: ACGATGCTGCAGGCAATGGT |
198 | |
| F: CGTTTGTATGATGTTGATCCAGAC |
343 | |
| F: CTACTCATTGCAGCATTTGC |
250 | |
| F: GCGAAAGTGAACTCTTATCTCTC |
480 | |
| F: TCTGTGTAGATGATGGTTCA |
250 | |
| F: TGATGACCGATACAACCTGT |
150 | |
| F: GGTAACGAATGTAGTGGTAG |
1,350 | |
| F: GCTACTTATCAAGTCCAAATC |
450 | |
| F: ACAACTTTGGTATCGTGGAAGG |
230 |