<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The present study aimed to determine the capsular serotype distribution and antimicrobial drug resistance patterns of <italic>Haemophilus influenzae</italic> from children in the Kunming region of China. This information could guide policymakers in clinical treatment. In the present study, <italic>H. influenzae</italic> isolates were tested for their serotypes, antimicrobial susceptibility pattern, and presence of β-lactamases. One-hundred forty-eight <italic>H. influenzae</italic> strains isolated from children 0–2 years old were investigated for capsular types by glass slide agglutination and molecular methods, and biotyped by the biochemical reactions. The drug resistance-encoding genes <italic>TEM-1</italic>, <italic>ROB-1</italic>, and the <italic>ftsI</italic> gene mutations <italic>PBP3-3</italic>, and <italic>PBP3-BLN</italic> were detected with real-time quantitative polymerase chain reaction (qPCR). The prevalence of β-lactamase-producing strains (60.3%) was significantly higher (<italic>p</italic> &lt; 0.05) than non-enzyme-producing strains. β-Lactamase-producing strains were multidrug resistant to various antibiotics such as ampicillin, tetracycline, sulfamethoxazole/trimethoprim, chloramphenicol, cefuroxime, and cefaclor. Among β-lactamase-producing strains, the detection rates of the <italic>TEM-1</italic>, <italic>PBP3-BLN</italic>, <italic>PBP3-s</italic>, and <italic>ROB-1</italic> were 54.1%, 18.9%, 11.8%, and 6.9%, respectively. The biotyping results show that most <italic>H. influenzae</italic> strains were of type II and III. Non-typeable <italic>H. influenza</italic>e (NTHi) accounted for 89.3% of the strains. NTHi strains were the most prevalent in this region; most belonged to biological types II and III. β-Lactamase-positive ampi-cillin-resistant (BLPAR) strains were prevalent among <italic>H. influenzae</italic> isolates in this region.</p>
</abstract>

The present study aimed to determine the capsular serotype distribution and antimicrobial drug resistance patterns of Haemophilus influenzae from children in the Kunming region of China. This information could guide policymakers in clinical treatment. In the present study, H. influenzae isolates were tested for their serotypes, antimicrobial susceptibility pattern, and presence of β-lactamases. One-hundred forty-eight H. influenzae strains isolated from children 0–2 years old were investigated for capsular types by glass slide agglutination and molecular methods, and biotyped by the biochemical reactions. The drug resistance-encoding genes TEM-1, ROB-1, and the ftsI gene mutations PBP3-3, and PBP3-BLN were detected with real-time quantitative polymerase chain reaction (qPCR). The prevalence of β-lactamase-producing strains (60.3%) was significantly higher (p < 0.05) than non-enzyme-producing strains. β-Lactamase-producing strains were multidrug resistant to various antibiotics such as ampicillin, tetracycline, sulfamethoxazole/trimethoprim, chloramphenicol, cefuroxime, and cefaclor. Among β-lactamase-producing strains, the detection rates of the TEM-1, PBP3-BLN, PBP3-s, and ROB-1 were 54.1%, 18.9%, 11.8%, and 6.9%, respectively. The biotyping results show that most H. influenzae strains were of type II and III. Non-typeable H. influenzae (NTHi) accounted for 89.3% of the strains. NTHi strains were the most prevalent in this region; most belonged to biological types II and III. β-Lactamase-positive ampi-cillin-resistant (BLPAR) strains were prevalent among H. influenzae isolates in this region.

Characterization of Serotypes and Molecular Drug Resistance Patterns of <italic>Haemophilus influenzae</italic> in Kunming Children

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characterization of Serotypes and Molecular Drug Resistance Patterns of  in Kunming Children

The present study aimed to determine the capsular serotype distribution and antimicrobial drug resistance patterns of Haemophilus influenzae from children in...

Characterization of Serotypes and Molecular Drug Resistance Patterns of Haemophilus influenzae in Kunming Children

Article Category: ORIGINAL PAPER

Published Online: Jun 14, 2023

Page range: 125 - 131

Received: Dec 11, 2022

Accepted: Jan 31, 2023

DOI: https://doi.org/10.33073/pjm-2023-006

Keywords
e, biotyping and capsule typing, resistant phenotype

© 2023 Mei Yuan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characterization of Serotypes and Molecular Drug Resistance Patterns of Haemophilus influenzae in Kunming Children

Article Category: ORIGINAL PAPER

Published Online: Jun 14, 2023

Page range: 125 - 131

Received: Dec 11, 2022

Accepted: Jan 31, 2023

DOI: https://doi.org/10.33073/pjm-2023-006

Keywordse, biotyping and capsule typing, resistant phenotype

© 2023 Mei Yuan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
e, biotyping and capsule typing, resistant phenotype