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Characterization of Serotypes and Molecular Drug Resistance Patterns of Haemophilus influenzae in Kunming Children

Mei Yuan

Mei Yuan

Department of Laboratory, Children’s Hospital affiliated with Kunming Medical UniversityKunming, China
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Yuan, Mei
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Mingbiao Ma

Mingbiao Ma

Department of Laboratory, Children’s Hospital affiliated with Kunming Medical UniversityKunming, China
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Ma, Mingbiao
, 
Hongchao Jiang

Hongchao Jiang

Science and Education Section, Children’s Hospital affiliated with Kunming Medical UniversityKunming, China
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Jiang, Hongchao
, 
Mao Fan

Mao Fan

Department of Laboratory, Children’s Hospital affiliated with Kunming Medical UniversityKunming, China
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Fan, Mao
, 
Ying Sun

Ying Sun

Epilepsy Center of Children, Children’s Hospital affiliated with Kunming Medical UniversityKunming, China
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Sun, Ying
, 
Bailing Zhou

Bailing Zhou

Department of Laboratory, Children’s Hospital affiliated with Kunming Medical UniversityKunming, China
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Zhou, Bailing
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Xingxing Feng

Xingxing Feng

Department of Laboratory, Children’s Hospital affiliated with Kunming Medical UniversityKunming, China
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Feng, Xingxing
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Junyi Yang

Junyi Yang

Department of Laboratory, Children’s Hospital affiliated with Kunming Medical UniversityKunming, China
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Yang, Junyi
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Min Su

Min Su

Department of Laboratory, Children’s Hospital affiliated with Kunming Medical UniversityKunming, China
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Su, Min
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Xiaoli He

Xiaoli He

Institute of Pediatrics, Children’s Hospital affiliated with Kunming Medical UniversityKunming, China
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He, Xiaoli
  
14 giu 2023
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Categoria dell'articolo: ORIGINAL PAPER
Pubblicato online: 14 giu 2023
Pagine: 125 - 131
Ricevuto: 11 dic 2022
Accettato: 31 gen 2023
DOI: https://doi.org/10.33073/pjm-2023-006
Parole chiave
© 2023 Mei Yuan et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Electrophoregram of Haemophilus influenzae capsular genes’ amplicons, including NT0Hi, Hie, Hib, Hif, and Hic strains.
Electrophoregram of Haemophilus influenzae capsular genes’ amplicons, including NT0Hi, Hie, Hib, Hif, and Hic strains.

Fig. 2.

Electrophoregram of Haemophilus influenzae drug-resistance genes (in the BLNAR and BLPACR phenotype strains).
Electrophoregram of Haemophilus influenzae drug-resistance genes (in the BLNAR and BLPACR phenotype strains).

The primers for drug resistance genes of Haemophilus influenzae (Søndergaard and Nørskov-Lauritsen 2016; Li et al_ 2020)_

Gene Primer Product size (bp)
TEM F: TAAGAGAATTATGCAGTGCTGCCR: TCCATAGTTGCCTGACTCCCC 458
ROB F: AAGCCCGCATTGGCGTTTATR: GGGCAATCGTCATGCCTTTG 521
PBP3S F: GATACTACGTCCTTTAAATTAAGR: GCAGTAAATGCCACATACTTA 551
PBP3BLN F: TTCAAGTAACCGTGGTGTGACR: GCAGTAAATGCCACATATTTC 465

Drug resistance rates of Haemophilus influenzae strains [n (%)]_

Antibiotic β-lactamase-positive (n = 474) β-lactamase-negative (n = 326) χ2 p
Ampicillin 474 (100.0) 113 (34.6) 45.725 ≤ 0.001***
Sulfamethoxazole/trimethoprim 399 (84.2) 232 (71.2) 14.558 ≤ 0.001***
Cefaclor 266 (56.1) 137 (42.0) 2.515 0.113
Cefuroxime 225 (47.5) 110 (33.8) 0.871 0.351
Amoxicillin/clavulanate 123 (25.9) 109 (33.4) 31.911 ≤ 0.001***
Tetracycline 48 (10.1) 12 (3.7) 3.266 0.071
Chloramphenicol 41 (8.6) 4 (1.2) 10.184 0.001***
Cefotaxime 4 (0.8) 5 (1.5) 1.593 0.207
Ofloxacin 2 (0.4) 1 (0.3) 0.010 0.919
Multidrug resistance 295 (62.2) 106 (32.5) 3.911 0.048*

The primers for capsular genes of Haemophilus influenzae (Fella et al_ 1994)_

Gene Primer Product size (bp)
P6 F: ACGATGCTGCAGGCAATGGTR: CATCAGTATTACCTTCTACTAAT 198
Hi (bexA) F: CGTTTGTATGATGTTGATCCAGACR: TGTCCATGTCTTCAAAATGATG 343
Hi-a F: CTACTCATTGCAGCATTTGCR: GAATATGACCTGATCTTCTG 250
Hi-b F: GCGAAAGTGAACTCTTATCTCTCR: GCTTACGCTTCTATCTCGGTGAA 480
Hi-c F: TCTGTGTAGATGATGGTTCAR: CAGAGGCAAGCTATTAGTGA 250
Hi-d F: TGATGACCGATACAACCTGTR: TCCACTCTTCAAACCATTCT 150
Hi-e F: GGTAACGAATGTAGTGGTAGR: GCTTTACTGTATAAGTCTAG 1,350
Hi-f F: GCTACTTATCAAGTCCAAATCR: CGCAATTATGGAAGAAAGCT 450
GAPDH F: ACAACTTTGGTATCGTGGAAGGR: GCCATCACGCCACAGTTTC 230
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