<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Listeria monocytogenes</italic> is a widespread foodborne pathogen contaminating foods during their production or processing stages. Fresh meat is susceptible to such contamination if it is not properly preserved. Our study was conducted to reveal the level of contamination and prevalence of <italic>Listeria</italic> spp. present in livestock and poultry meat from Gansu province. A total of 1,387 samples were collected from five cities in Gansu Province according to standard sampling procedures, of which 174 samples (12.5%) were positive for <italic>Listeria</italic> species. Among them, 14 isolates of <italic>L. monocytogenes</italic> (1.0%), 150 isolates of <italic>Listeria innocua</italic> (10.8%), and ten isolates of <italic>Listeria welshimeri</italic> (0.7%) were identified by conventional bacteriological and molecular identification methods. All isolates were subjected to serological assays, antimicrobial susceptibility tests, growth curve assays, determination of biofilm-forming capacity, and cluster analysis of the 16S rRNA gene sequences. Four predominant serotypes of <italic>L. monocytogenes</italic> were identified, including 1/2a (35.7%, 5/14), 1/2b (14.3%, 2/14), 1/2c (42.9%, 6/14), and 4b (7.1%, 1/14). All <italic>L. monocytogenes</italic> isolates were resistant to tetracycline and cefoxitin. Most <italic>L. innocua</italic> isolates (63.6%, 14/22) and <italic>L. welshimeri</italic> (40%, 4/10) were resistant to tetracycline. The high biofilm-forming ability was observed among 1/2c and 1/2a serotype isolates. The cluster analysis of the 16S rRNA gene sequences revealed a close genetic relationship between the three <italic>Listeria</italic> species. This study fills the gap in the knowledge of livestock and poultry meat that carry <italic>Listeria</italic> in slaughterhouses and markets in Gansu Province.<fig id="j_pjm-2023-002_ufig_001" position="float" fig-type="figure">
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/j_pjm-2023-002_ufig_001.jpg"></graphic>
</fig></p>
</abstract>

Listeria monocytogenes is a widespread foodborne pathogen contaminating foods during their production or processing stages. Fresh meat is susceptible to such contamination if it is not properly preserved. Our study was conducted to reveal the level of contamination and prevalence of Listeria spp. present in livestock and poultry meat from Gansu province. A total of 1,387 samples were collected from five cities in Gansu Province according to standard sampling procedures, of which 174 samples (12.5%) were positive for Listeria species. Among them, 14 isolates of L. monocytogenes (1.0%), 150 isolates of Listeria innocua (10.8%), and ten isolates of Listeria welshimeri (0.7%) were identified by conventional bacteriological and molecular identification methods. All isolates were subjected to serological assays, antimicrobial susceptibility tests, growth curve assays, determination of biofilm-forming capacity, and cluster analysis of the 16S rRNA gene sequences. Four predominant serotypes of L. monocytogenes were identified, including 1/2a (35.7%, 5/14), 1/2b (14.3%, 2/14), 1/2c (42.9%, 6/14), and 4b (7.1%, 1/14). All L. monocytogenes isolates were resistant to tetracycline and cefoxitin. Most L. innocua isolates (63.6%, 14/22) and L. welshimeri (40%, 4/10) were resistant to tetracycline. The high biofilm-forming ability was observed among 1/2c and 1/2a serotype isolates. The cluster analysis of the 16S rRNA gene sequences revealed a close genetic relationship between the three Listeria species. This study fills the gap in the knowledge of livestock and poultry meat that carry Listeria in slaughterhouses and markets in Gansu Province.

Prevalence and Biological Characteristics of <italic>Listeria</italic> Species Isolated from Livestock and Poultry Meat in Gansu Province, China

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Prevalence and Biological Characteristics of  Species Isolated from Livestock and Poultry Meat in Gansu Province, China

Listeria monocytogenes is a widespread foodborne pathogen contaminating foods during their production or processing stages. Fresh meat is susceptible to such...

Prevalence and Biological Characteristics of Listeria Species Isolated from Livestock and Poultry Meat in Gansu Province, China

Article Category: ORIGINAL PAPER

Published Online: Mar 24, 2023

Page range: 11 - 20

Received: Oct 06, 2022

Accepted: Dec 27, 2022

DOI: https://doi.org/10.33073/pjm-2023-002

Keywords
livestock and poultry meat, slaughtering, cluster analysis

© 2023 ZHIJIE DONG et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Supplementary Material Details

Prevalence and Biological Characteristics of Listeria Species Isolated from Livestock and Poultry Meat in Gansu Province, China

Article Category: ORIGINAL PAPER

Published Online: Mar 24, 2023

Page range: 11 - 20

Received: Oct 06, 2022

Accepted: Dec 27, 2022

DOI: https://doi.org/10.33073/pjm-2023-002

Keywordslivestock and poultry meat, slaughtering, cluster analysis

© 2023 ZHIJIE DONG et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Supplementary Material Details

Keywords
livestock and poultry meat, slaughtering, cluster analysis