Open Access

Breeding of High Daptomycin-Producing Strain by Streptomycin Resistance Superposition

Shuaibei Chu

Shuaibei Chu

College of Life Science and Agricultural Engineering, Nanyang Normal UniversityNanyang, China
Orcid profile
Search for this author on
Sciendo|Google Scholar
Chu, Shuaibei
, 
Wenting Hu

Wenting Hu

College of Life Science and Agricultural Engineering, Nanyang Normal UniversityNanyang, China
Search for this author on
Sciendo|Google Scholar
Hu, Wenting
, 
Kaihong Zhang

Kaihong Zhang

College of Life Science and Agricultural Engineering, Nanyang Normal UniversityNanyang, China
Search for this author on
Sciendo|Google Scholar
Zhang, Kaihong
 and 
Fengli Hui

Fengli Hui

  • College of Life Science and Agricultural Engineering, Nanyang Normal UniversityNanyang, China
  • Research Center of Henan Provincial Agricultural Biomass Resource Engineering and TechnologyNanyang, China
Search for this author on
Sciendo|Google Scholar
Hui, Fengli
  
Sep 24, 2022
Breeding of High Daptomycin-Producing Strain by Streptomycin Resistance Superposition's Cover Image
About this article
Previous Article
Next Article
Cite
Download Cover
Article Category: Original Paper
Published Online: Sep 24, 2022
Page range: 463 - 471
Received: May 05, 2022
Accepted: Aug 02, 2022
DOI: https://doi.org/10.33073/pjm-2022-041
Keywords
© 2022 Shuaibei Chu et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
undefined

Fig. 1

The growth of Streptomyces roseosporus original strain on DT plates with a) 0 μg/ml, b) 0.8 μg/ml, c) 1.0 μg/ml, d) 1.2 μg/ml, e) 1.4 μg/ml streptomycin, respectively.
The growth of Streptomyces roseosporus original strain on DT plates with a) 0 μg/ml, b) 0.8 μg/ml, c) 1.0 μg/ml, d) 1.2 μg/ml, e) 1.4 μg/ml streptomycin, respectively.

Fig. 2

Daptomycin production by Streptomyces roseosporus mutants screened by DT plates with streptomycin.at a concentration of a) 6 μg/ml, b) 1.8 μg/ml, c) 2.0 μg/ml, d) 2.2 μg/ml, e) 2.4 μg/ml, f) 2.6 μg/ml, g) 2.8 μg/ml, and h) 3.0 μg/ml. The dashed line represents daptomycin production of the parent strain.
Daptomycin production by Streptomyces roseosporus mutants screened by DT plates with streptomycin.at a concentration of a) 6 μg/ml, b) 1.8 μg/ml, c) 2.0 μg/ml, d) 2.2 μg/ml, e) 2.4 μg/ml, f) 2.6 μg/ml, g) 2.8 μg/ml, and h) 3.0 μg/ml. The dashed line represents daptomycin production of the parent strain.

Fig. 3

a1) and b1) Colony and individual morphology of Streptomyces roseosporus strains BNCC 342432 and SR-2620; a2) and b2) spore growth capacity of BNCC 342432 and SR-2620; a3) and b3) mycelial pellet morphology of BNCC 342432 and SR-2620.
a1) and b1) Colony and individual morphology of Streptomyces roseosporus strains BNCC 342432 and SR-2620; a2) and b2) spore growth capacity of BNCC 342432 and SR-2620; a3) and b3) mycelial pellet morphology of BNCC 342432 and SR-2620.

Fig. 4

Sequence comparison of the rpsL gene between Streptomyces roseosporus BNCC 342432 and SR-2620 strains.
Sequence comparison of the rpsL gene between Streptomyces roseosporus BNCC 342432 and SR-2620 strains.

Fig. 5

Comparison of shake flask fermentation between Streptomyces roseosporus BNCC 342432 and SR-2620 strains.
Comparison of shake flask fermentation between Streptomyces roseosporus BNCC 342432 and SR-2620 strains.

Stability experiment on high-yield daptomycin strains_

Strain First generation of daptomycin production (mg/l) Second generation of daptomycin production (mg/l) Third generation of daptomycin production (mg/l) Fourth generation of daptomycin production (mg/l) Fifth generation of daptomycin production (mg/l)
BNCC 342432 10.5 ± 0.2 9.9 ± 0.2 10.3 ± 0.2 9.7 ± 0.2 10.4 ± 0.2
SR-2009 28.5 ± 0.2 30.0 ± 0.2 24.3 ± 0.2 25.0 ± 0.2 22.8 ± 0.2
SR-2017 23.1 ± 0.2 21.6 ± 0.2 22.8 ± 0.2 19.8 ± 0.2 14.6 ± 0.2
SR-2023 29.7 ± 0.2 28.8 ± 0.2 30.6 ± 0.2 29.2 ± 0.2 27.2 ± 0.2
SR-2026 24.2 ± 0.2 22.9 ± 0.2 21.8 ± 0.2 20.1 ± 0.2 20.6 ± 0.2
SR-2401 24.5 ± 0.2 25.5 ± 0.2 23.4 ± 0.2 22.5 ± 0.2 24.2 ± 0.2
SR-2620 38.5 ± 0.2 36.9 ± 0.2 41.3 ± 0.2 36.0 ± 0.2 38.5 ± 0.2
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Microbiology and Virology
Journal RSS Feed