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Breeding of High Daptomycin-Producing Strain by Streptomycin Resistance Superposition

Shuaibei Chu
Shuaibei Chu
, 
Wenting Hu
Wenting Hu
, 
Kaihong Zhang
Kaihong Zhang
 y 
Fengli Hui
Fengli Hui
   | 24 sept 2022
Polish Journal of Microbiology's Cover Image
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Article Category: Original Paper
Publicado en línea: 24 sept 2022
Páginas: 463 - 471
Recibido: 05 may 2022
Aceptado: 02 ago 2022
DOI: https://doi.org/10.33073/pjm-2022-041
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© 2022 Shuaibei Chu et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1

The growth of Streptomyces roseosporus original strain on DT plates with a) 0 μg/ml, b) 0.8 μg/ml, c) 1.0 μg/ml, d) 1.2 μg/ml, e) 1.4 μg/ml streptomycin, respectively.
The growth of Streptomyces roseosporus original strain on DT plates with a) 0 μg/ml, b) 0.8 μg/ml, c) 1.0 μg/ml, d) 1.2 μg/ml, e) 1.4 μg/ml streptomycin, respectively.

Fig. 2

Daptomycin production by Streptomyces roseosporus mutants screened by DT plates with streptomycin.at a concentration of a) 6 μg/ml, b) 1.8 μg/ml, c) 2.0 μg/ml, d) 2.2 μg/ml, e) 2.4 μg/ml, f) 2.6 μg/ml, g) 2.8 μg/ml, and h) 3.0 μg/ml. The dashed line represents daptomycin production of the parent strain.
Daptomycin production by Streptomyces roseosporus mutants screened by DT plates with streptomycin.at a concentration of a) 6 μg/ml, b) 1.8 μg/ml, c) 2.0 μg/ml, d) 2.2 μg/ml, e) 2.4 μg/ml, f) 2.6 μg/ml, g) 2.8 μg/ml, and h) 3.0 μg/ml. The dashed line represents daptomycin production of the parent strain.

Fig. 3

a1) and b1) Colony and individual morphology of Streptomyces roseosporus strains BNCC 342432 and SR-2620; a2) and b2) spore growth capacity of BNCC 342432 and SR-2620; a3) and b3) mycelial pellet morphology of BNCC 342432 and SR-2620.
a1) and b1) Colony and individual morphology of Streptomyces roseosporus strains BNCC 342432 and SR-2620; a2) and b2) spore growth capacity of BNCC 342432 and SR-2620; a3) and b3) mycelial pellet morphology of BNCC 342432 and SR-2620.

Fig. 4

Sequence comparison of the rpsL gene between Streptomyces roseosporus BNCC 342432 and SR-2620 strains.
Sequence comparison of the rpsL gene between Streptomyces roseosporus BNCC 342432 and SR-2620 strains.

Fig. 5

Comparison of shake flask fermentation between Streptomyces roseosporus BNCC 342432 and SR-2620 strains.
Comparison of shake flask fermentation between Streptomyces roseosporus BNCC 342432 and SR-2620 strains.

Stability experiment on high-yield daptomycin strains.

Strain First generation of daptomycin production (mg/l) Second generation of daptomycin production (mg/l) Third generation of daptomycin production (mg/l) Fourth generation of daptomycin production (mg/l) Fifth generation of daptomycin production (mg/l)
BNCC 342432 10.5 ± 0.2 9.9 ± 0.2 10.3 ± 0.2 9.7 ± 0.2 10.4 ± 0.2
SR-2009 28.5 ± 0.2 30.0 ± 0.2 24.3 ± 0.2 25.0 ± 0.2 22.8 ± 0.2
SR-2017 23.1 ± 0.2 21.6 ± 0.2 22.8 ± 0.2 19.8 ± 0.2 14.6 ± 0.2
SR-2023 29.7 ± 0.2 28.8 ± 0.2 30.6 ± 0.2 29.2 ± 0.2 27.2 ± 0.2
SR-2026 24.2 ± 0.2 22.9 ± 0.2 21.8 ± 0.2 20.1 ± 0.2 20.6 ± 0.2
SR-2401 24.5 ± 0.2 25.5 ± 0.2 23.4 ± 0.2 22.5 ± 0.2 24.2 ± 0.2
SR-2620 38.5 ± 0.2 36.9 ± 0.2 41.3 ± 0.2 36.0 ± 0.2 38.5 ± 0.2
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