Open Access

Structural and Dynamic Analysis of Leaf-Associated Fungal Community of Walnut Leaves Infected by Leaf Spot Disease Based Illumina High-Throughput Sequencing Technology

Shiwei Wang

Shiwei Wang

College of Forestry, Sichuan Agricultural UniversityChengdu, China
Search for this author on
Sciendo|Google Scholar
Wang, Shiwei
, 
Yu Tan

Yu Tan

  • College of Forestry, Sichuan Agricultural UniversityChengdu, China
  • Chengdu Botanical GardenChengdu, China
Search for this author on
Sciendo|Google Scholar
Tan, Yu
, 
Shujiang Li

Shujiang Li

  • College of Forestry, Sichuan Agricultural UniversityChengdu, China
  • National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze RiverChengdu, China
Search for this author on
Sciendo|Google Scholar
Li, Shujiang
 and 
Tianhui Zhu

Tianhui Zhu

College of Forestry, Sichuan Agricultural UniversityChengdu, China
Orcid profile
Search for this author on
Sciendo|Google Scholar
Zhu, Tianhui
  
Sep 24, 2022
Structural and Dynamic Analysis of Leaf-Associated Fungal Community of Walnut Leaves Infected by Leaf Spot Disease Based Illumina High-Throughput Sequencing Technology's Cover Image
About this article
Previous Article
Next Article
Cite
Download Cover
Article Category: Original Paper
Published Online: Sep 24, 2022
Page range: 429 - 441
Received: Apr 01, 2022
Accepted: Jul 25, 2022
DOI: https://doi.org/10.33073/pjm-2022-038
Keywords
© 2022 Shiwei Wang et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
undefined

Fig. 1

Line chart of alpha diversity index for different sampling times; a) dynamic changes of Chao1 index, b) dynamic changes of Shannon index. The different letters indicate the significant difference at the 0.05 level (p < 0.05, n = 5).
Line chart of alpha diversity index for different sampling times; a) dynamic changes of Chao1 index, b) dynamic changes of Shannon index. The different letters indicate the significant difference at the 0.05 level (p < 0.05, n = 5).

Fig. 2

Column map of species relative abundance at phylum level (Group Analysis).
Column map of species relative abundance at phylum level (Group Analysis).

Fig. 3

The group’s phylogenetic relationship of genus species at the level of analysis. A phylogenetic tree constructed from representative sequences of genus levels. The color of the branches and sectors represents the corresponding phylum. The stacked column diagram outside the fan ring illustrates the abundance distribution information of the genus in different samples.
The group’s phylogenetic relationship of genus species at the level of analysis. A phylogenetic tree constructed from representative sequences of genus levels. The color of the branches and sectors represents the corresponding phylum. The stacked column diagram outside the fan ring illustrates the abundance distribution information of the genus in different samples.

Fig. 4

Species relative abundance of species at the genus level (by group analysis); a) relative abundance of the top 10 genera, b) relative abundance of the top 30 genera.
Species relative abundance of species at the genus level (by group analysis); a) relative abundance of the top 10 genera, b) relative abundance of the top 30 genera.

Fig. 5

Heat map of the Genus-level species abundance clustering (by group analysis). The longitudinal direction is the sample information, and the horizontal direction is the species annotation information. The cluster tree on the left side of figure is the species clustering tree. Different colors represent different relative abundances, red represents the high relative abundance, and blue represents the low relative abundance.
Heat map of the Genus-level species abundance clustering (by group analysis). The longitudinal direction is the sample information, and the horizontal direction is the species annotation information. The cluster tree on the left side of figure is the species clustering tree. Different colors represent different relative abundances, red represents the high relative abundance, and blue represents the low relative abundance.

Fig. 6

Line chart of relative abundance for different samples; a) Relative abundance of Colletotrichum spp. at different sampling times, b) relative abundance of Fusarium spp. at different sampling times, c) relative abundance of Alternaria spp. at different sampling times, d) relative abundance of Colletotrichum spp., Fusarium spp., and Alternaria spp. The different letters indicate the significant difference at the 0.05 level (p < 0.05, n = 5).
Line chart of relative abundance for different samples; a) Relative abundance of Colletotrichum spp. at different sampling times, b) relative abundance of Fusarium spp. at different sampling times, c) relative abundance of Alternaria spp. at different sampling times, d) relative abundance of Colletotrichum spp., Fusarium spp., and Alternaria spp. The different letters indicate the significant difference at the 0.05 level (p < 0.05, n = 5).

Fig. 7

Phyllosphere fungal assemblage dissimilarity among healthy (HE1 ~ 5) and infected (IN1 ~ 5) leaves, represented by nonmetric multidimensional scaling (NMDS).
Phyllosphere fungal assemblage dissimilarity among healthy (HE1 ~ 5) and infected (IN1 ~ 5) leaves, represented by nonmetric multidimensional scaling (NMDS).

The non-parametric Wilcox test results_

The non-parametric Wilcox test results of Chao1 index
Difference p-value sig. LCL UCL
HE1-IN1   –4.6 0.3909 –15.3189   6.118942
HE2-IN2    1.2 0.8221 –9.51894 11.91894
HE3-IN3   –8.6 0.1128 –19.3189   2.118942
HE4-IN4   –9.8 0.072 –20.5189   0.918942
HE5-IN5 –20 0.0005 *** –30.7189 –9.28106
The non-parametric Wilcox test results of Shannon index
Difference p-value sig. LCL UCL
HE1-IN1   –6.8 0.1353 –15.8168   2.216805
HE2-IN2 –17.8 0.0003 *** –26.8168 –8.78319
HE3-IN3 –26.2 0 *** –35.2168 –17.1832
HE4-IN4 –12.4 0.0083 ** –21.4168 –3.38319
HE5-IN5   –8.2 0.0735 –17.2168   0.816805
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Microbiology and Virology
Journal RSS Feed