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Expression Level of the mip, pmp18D, and ompA Genes in Chlamydia abortus Isolated from Aborted Ewes

Eman Dhahir Arif

Eman Dhahir Arif

Department of Microbiology, College of Veterinary Medicine, University of SulaimaniSulaymaniyah, Iraq
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Arif, Eman Dhahir
, 
Nahla Mohammad Saeed

Nahla Mohammad Saeed

Department of Microbiology, College of Veterinary Medicine, University of SulaimaniSulaymaniyah, Iraq
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Saeed, Nahla Mohammad
, 
Shwan Kamal Rachid

Shwan Kamal Rachid

Charmo UniversitySulaymaniyah, Iraq
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Rachid, Shwan Kamal
, 
Hiewa Othman Dyary

Hiewa Othman Dyary

Department of Basic Sciences, College of Veterinary Medicine, University of SulaimaniSulaymaniyah, Iraq
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Dyary, Hiewa Othman
 and 
Peshnyar M.A. Rashid

Peshnyar M.A. Rashid

  • Kurdistan Institution for Strategic Studies and Scientific ResearchSulaymaniyah, Iraq
  • Molecular Diagnostic Laboratory, Directorate of Veterinary Services in SulaymaniyahSulaymaniyah, Iraq
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Rashid, Peshnyar M.A.
  
Mar 30, 2022
Expression Level of the mip, pmp18D, and ompA Genes in Chlamydia abortus Isolated from Aborted Ewes's Cover Image
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Article Category: Original Paper
Published Online: Mar 30, 2022
Page range: 115 - 121
Received: Nov 04, 2021
Accepted: Feb 20, 2022
DOI: https://doi.org/10.33073/pjm-2022-014
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© 2022 Eman Dhahir Arif et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1

mRNA expression levels of the ompA, pmp18D, and mip genes as fold change in pregnant and nonpregnant mice tissues at different pregnancy times by RT-qPCR. Data are presented as means of values for six mice (columns) ± SEM (error bars).
mRNA expression levels of the ompA, pmp18D, and mip genes as fold change in pregnant and nonpregnant mice tissues at different pregnancy times by RT-qPCR. Data are presented as means of values for six mice (columns) ± SEM (error bars).

Sequences of the forward and reverse primers of four genes used in Real-time PCR_

Target gene Primer sequence (5′ to 3′) Size (bp)
GAPDH Forward: GGGGTCCCAGCTTAGGTTCAReverse: ACGGCCAAATCCGTTCACA 95
mip Forward: AAGAAAACCTCTCCCTAGCCReverse: CTGAAGGTTTCCCTGATATTG 139
ompA Forward: GCGGCATTCAACCTCGTTReverse: CCTTGAGTGATGCCTACATTGG 85
pmp18D Forward: TCCACTGGGATGATCACCAATAReverse: GCATAGAAAGCGTATCGAGAATCAC 81

Wilcoxon signed-rank test analysis of the mip, pmp18D, and ompA genes expression between the pregnant groups in comparison to the nonpregnant groups at each time interval of pregnancy_

Group Gene type Period of pregnancy (days) p-values
Pregnant group – nonpregnant mip 101520 0.5930.6550.655
Pregnant group – nonpregnant pmp18D 101520 0.1090.5930.593
Pregnant group – nonpregnant ompA 101520 0.2851.0000.655
Language:
English
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4 times per year
Journal Subjects:
Life Sciences, Microbiology and Virology
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