<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Staphylococcus aureus</italic> is the causative agent of numerous and varied clinical infections. Crude aqueous extracts of <italic>Melia azedarach</italic> fruits inhibit the planktonic growth and initial biofilm formation of <italic>S. aureus</italic> in a dose-dependent manner. Moreover, the biofilm topologies became sparse and decreased as the concentration of the aqueous extracts increased. RNA-Seq analyses revealed 532 differentially expressed genes (DEGs) after <italic>S. aureus</italic> exposure to 0.25 g/ml extracts; 319 of them were upregulated, and 213 were downregulated. The majority of DEGs were categorized into abundant sub-groups in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, untargeted UHPLC-MS/MS analyses of the aqueous extracts of <italic>M. azedarach</italic> fruits demonstrated a highly complex profile in positive and negative electrospray ionization modes. The extracts primarily consisted of lipids and lipid-like molecules, organic acids and their derivatives, phenylpropanoids, polyketides, organoheterocyclic compounds, and benzenoids annotated by abundant lipid maps and KEGG pathways. Overall, this study provides evidences that the aqueous extracts of <italic>M. azedarach</italic> fruits can control <italic>S. aureus</italic> infections and sought to understand the mode of action of these extracts on <italic>S. aureus</italic>.</p></abstract>

Staphylococcus aureus is the causative agent of numerous and varied clinical infections. Crude aqueous extracts of Melia azedarach fruits inhibit the planktonic growth and initial biofilm formation of S. aureus in a dose-dependent manner. Moreover, the biofilm topologies became sparse and decreased as the concentration of the aqueous extracts increased. RNA-Seq analyses revealed 532 differentially expressed genes (DEGs) after S. aureus exposure to 0.25 g/ml extracts; 319 of them were upregulated, and 213 were downregulated. The majority of DEGs were categorized into abundant sub-groups in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, untargeted UHPLC-MS/MS analyses of the aqueous extracts of M. azedarach fruits demonstrated a highly complex profile in positive and negative electrospray ionization modes. The extracts primarily consisted of lipids and lipid-like molecules, organic acids and their derivatives, phenylpropanoids, polyketides, organoheterocyclic compounds, and benzenoids annotated by abundant lipid maps and KEGG pathways. Overall, this study provides evidences that the aqueous extracts of M. azedarach fruits can control S. aureus infections and sought to understand the mode of action of these extracts on S. aureus.

Chemical Components of Aqueous Extracts of <italic>Melia azedarach</italic> Fruits and Their Effects on The Transcriptome of <italic>Staphylococcus aureus</italic>

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Chemical Components of Aqueous Extracts of  Fruits and Their Effects on The Transcriptome of 

Staphylococcus aureus is the causative agent of numerous and varied clinical infections. Crude aqueous extracts of Melia azedarach fruits inhibit the...

Chemical Components of Aqueous Extracts of Melia azedarach Fruits and Their Effects on The Transcriptome of Staphylococcus aureus

Article Category: original-paper

Published Online: Oct 29, 2021

Page range: 447 - 459

Received: Jun 20, 2021

Accepted: Sep 15, 2021

DOI: https://doi.org/10.33073/pjm-2021-041

Keywords
fruits, differentially expressed genes, biofilms, UHPLC-MS/MS

© 2021 Hong Peng et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Chemical Components of Aqueous Extracts of Melia azedarach Fruits and Their Effects on The Transcriptome of Staphylococcus aureus

Article Category: original-paper

Published Online: Oct 29, 2021

Page range: 447 - 459

Received: Jun 20, 2021

Accepted: Sep 15, 2021

DOI: https://doi.org/10.33073/pjm-2021-041

Keywordsfruits, differentially expressed genes, biofilms, UHPLC-MS/MS

© 2021 Hong Peng et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
fruits, differentially expressed genes, biofilms, UHPLC-MS/MS