<abstract xmlns="http://www.w3.org/1999/xhtml">Ligninolytic bacteria degrading lignin were isolates and identified, and their biodegradation mechanism of alkaline-lignin was investigated. Four strains with lignin degradation capability were screened and identified from the soil, straw, and silage based on their decolorizing capacity of aniline blue and colony size on alkaline-lignin medium. The degradation ratio of <italic>Bacillus aryabhattai</italic> BY5, <italic>Acinetobacter johnsonii</italic> LN2, <italic>Acinetobacter lwoffii</italic> LN4, and <italic>Micrococcus yunnanensis</italic> CL32 have been assayed using alkaline-lignin as the unique carbon source. Further, the Lip (lignin peroxidase) and Mnp (manganese peroxidase) activities of strains were investigated. Lip activity of <italic>A. lwoffii</italic> LN4 was highest after 72 h of incubation and reached 7151.7 U · l–1. Mnp activity of <italic>M. yunnanensis</italic> CL32 was highest after 48 h and reached 12533 U · l–1. The analysis of alkaline-lignin degradation products by GC-MS revealed that the strains screened could utilize aromatic esters compounds such as dibutyl phthalate (DBP), and decomposite monocyclic aromatic compounds through the DBP aerobic metabolic pathway. The results indicate that <italic>B. aryabhattai</italic> BY5, <italic>A. johnsonii</italic> LN2, <italic>A. lwoffii</italic> LN4, and <italic>M. yunnanensis</italic> CL32 have high potential to degrade alkaline-lignin, and might utilize aromatic compounds by DBP aerobic metabolic pathway in the process of lignin degradation.</abstract>

Ligninolytic bacteria degrading lignin were isolates and identified, and their biodegradation mechanism of alkaline-lignin was investigated. Four strains with lignin degradation capability were screened and identified from the soil, straw, and silage based on their decolorizing capacity of aniline blue and colony size on alkaline-lignin medium. The degradation ratio of Bacillus aryabhattai BY5, Acinetobacter johnsonii LN2, Acinetobacter lwoffii LN4, and Micrococcus yunnanensis CL32 have been assayed using alkaline-lignin as the unique carbon source. Further, the Lip (lignin peroxidase) and Mnp (manganese peroxidase) activities of strains were investigated. Lip activity of A. lwoffii LN4 was highest after 72 h of incubation and reached 7151.7 U · l–1. Mnp activity of M. yunnanensis CL32 was highest after 48 h and reached 12533 U · l–1. The analysis of alkaline-lignin degradation products by GC-MS revealed that the strains screened could utilize aromatic esters compounds such as dibutyl phthalate (DBP), and decomposite monocyclic aromatic compounds through the DBP aerobic metabolic pathway. The results indicate that B. aryabhattai BY5, A. johnsonii LN2, A. lwoffii LN4, and M. yunnanensis CL32 have high potential to degrade alkaline-lignin, and might utilize aromatic compounds by DBP aerobic metabolic pathway in the process of lignin degradation.

Characterization of Ligninolytic Bacteria and Analysis of Alkali-Lignin Biodegradation Products

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Ligninolytic bacteria degrading lignin were isolates and identified, and their biodegradation mechanism of alkaline-lignin was investigated. Four strains with...

Characterization of Ligninolytic Bacteria and Analysis of Alkali-Lignin Biodegradation Products

Article Category: original-paper

Published Online: Sep 09, 2020

Page range: 339 - 347

Received: May 25, 2020

Accepted: Aug 11, 2020

DOI: https://doi.org/10.33073/pjm-2020-037

Keywords
isolation, bacteria, alkali-lignin, biodegradation products

© 2020 Yi Xiong et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Supplementary Material Details

Characterization of Ligninolytic Bacteria and Analysis of Alkali-Lignin Biodegradation Products

Article Category: original-paper

Published Online: Sep 09, 2020

Page range: 339 - 347

Received: May 25, 2020

Accepted: Aug 11, 2020

DOI: https://doi.org/10.33073/pjm-2020-037

Keywordsisolation, bacteria, alkali-lignin, biodegradation products

© 2020 Yi Xiong et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Supplementary Material Details

Keywords
isolation, bacteria, alkali-lignin, biodegradation products