<abstract xmlns="http://www.w3.org/1999/xhtml"><italic>Rhizoctonia solani</italic> is a soil-borne fungus causing a wide range of plants diseases. <italic>Trichoderma gamsii</italic> strain T30 has previously been reported as antagonistic against <italic>R. solani</italic>. Although there are a few studies about the influence of <italic>Trichoderma</italic> strains on the <italic>R. solani</italic> density in a pathosystem in the presence of plant hosts, this report for the first time comprehensively describes <italic>in situ</italic> effects of a <italic>T. gamsii</italic> strain on the population density of <italic>R. solani</italic> in the soil microcosmic conditions. The population dynamics of <italic>R. solani</italic> were followed in the autoclaved and non-autoclaved soils in artificially prepared microcosms up to day 25 after co-inoculation with <italic>T. gamsii</italic> in the variable ratios (R1/T1; R1/T0.1; R1/T0.01 of <italic>R. solani</italic>/<italic>T. gamsii</italic>). The population density of <italic>R. solani</italic> was evaluated by qPCR. In the autoclaved soil, target DNA copies of <italic>R. solani</italic> increased in the control samples from 1 × 105 to 6.5 × 106. At R1/T0.01, the number of target DNA copies were not significantly changed until day 11; however, it decreased by around five times at day 25. At R1/T0.1 and R1/T1, the number of DNA copies was reduced to 2.1 × 106 and 7.6 × 105 at day 11, respectively and the reduction was as much as 17 times at day 25. In the non-autoclaved soil, the number of the fungal cells decreased at day 25 whether inoculated or not with <italic>Trichoderma</italic> indicating a general suppression by the soil microbiome. In brief, <italic>T. gamsii</italic> significantly inhibited the growth of <italic>R. solani</italic> in the soil <italic>in situ</italic> and there was a general suppressive effect of the natural microbiome.</abstract>

Rhizoctonia solani is a soil-borne fungus causing a wide range of plants diseases. Trichoderma gamsii strain T30 has previously been reported as antagonistic against R. solani. Although there are a few studies about the influence of Trichoderma strains on the R. solani density in a pathosystem in the presence of plant hosts, this report for the first time comprehensively describes in situ effects of a T. gamsii strain on the population density of R. solani in the soil microcosmic conditions. The population dynamics of R. solani were followed in the autoclaved and non-autoclaved soils in artificially prepared microcosms up to day 25 after co-inoculation with T. gamsii in the variable ratios (R1/T1; R1/T0.1; R1/T0.01 of R. solani/T. gamsii). The population density of R. solani was evaluated by qPCR. In the autoclaved soil, target DNA copies of R. solani increased in the control samples from 1 × 105 to 6.5 × 106. At R1/T0.01, the number of target DNA copies were not significantly changed until day 11; however, it decreased by around five times at day 25. At R1/T0.1 and R1/T1, the number of DNA copies was reduced to 2.1 × 106 and 7.6 × 105 at day 11, respectively and the reduction was as much as 17 times at day 25. In the non-autoclaved soil, the number of the fungal cells decreased at day 25 whether inoculated or not with Trichoderma indicating a general suppression by the soil microbiome. In brief, T. gamsii significantly inhibited the growth of R. solani in the soil in situ and there was a general suppressive effect of the natural microbiome.

<italic>In situ</italic> Impact of the Antagonistic Fungal Strain, <italic>Trichoderma gamsii</italic> T30 on the Plant Pathogenic Fungus, <italic>Rhizoctonia solani</italic> in Soil

Polish Journal of Microbiology

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 Impact of the Antagonistic Fungal Strain,  T30 on the Plant Pathogenic Fungus,  in Soil

Rhizoctonia solani is a soil-borne fungus causing a wide range of plants diseases. Trichoderma gamsii strain T30 has previously been reported as antagonistic...

In situ Impact of the Antagonistic Fungal Strain, Trichoderma gamsii T30 on the Plant Pathogenic Fungus, Rhizoctonia solani in Soil

Article Category: original-paper

Published Online: Jun 28, 2019

Page range: 211 - 216

Received: Oct 14, 2018

Accepted: Feb 12, 2019

DOI: https://doi.org/10.33073/pjm-2019-021

Keywords
study, population density, antagonism, real-time PCR

© 2019 Muhammad Anees et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

In situ Impact of the Antagonistic Fungal Strain, Trichoderma gamsii T30 on the Plant Pathogenic Fungus, Rhizoctonia solani in Soil

Article Category: original-paper

Published Online: Jun 28, 2019

Page range: 211 - 216

Received: Oct 14, 2018

Accepted: Feb 12, 2019

DOI: https://doi.org/10.33073/pjm-2019-021

Keywordsstudy, population density, antagonism, real-time PCR

© 2019 Muhammad Anees et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
study, population density, antagonism, real-time PCR