<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The present study aimed to isolate the high-efficiency petrol metabolizing thermophilic bacteria from petrol contaminated soil samples. Isolation was carried out through enrichment culture, serial dilution and pour plate methods using the petrol supplemented minimal salt media. The isolated bacteria were analyzed to document growth behavior, petrol removal efficiencies, antibiotic resistance profile, and biochemical characteristics. The 16S rRNA based phylogenetic analysis helped to reveal the identity of isolated bacterial species and construct the phylogenetic trees. Total nine bacteria were isolated, out of which three (IUBP2, IUBP3, IUBP5) were identified as <italic>Brevibacillus formosus</italic>, one (IUBP1) was found similar to <italic>Brevibacillus agri</italic>, four (IUBP7, IUBP8, IUBP13, and IUBP14) shared homology with <italic>Burkholderia lata</italic>, and one (IUBP15) with <italic>Burkholderia pyrrocinia</italic>. All the isolates were fast growing and exhibited considerable petrol degradation potential. The highest petrol removal efficiency (69.5% ± 13.44/6 days) was recorded for the strain IUBP15 at a petrol concentration of 0.1% (v/v). All bacteria studied (100%) were positive for esculinase and phosphatase. Many strains exhibited positive responses for arginine dehydrolase (22%), <italic>β</italic>-naphthylamidase (11%), <italic>β</italic>-D-glucosaminide (33%), mannitol (55%), sorbitol (66%) and inulin (88%) fermentation test. While all were sensitive to the antibiotics, some of them were found resistant against chloramphenicol and oxacillin. The remarkable biochemical characteristics and considerable petrol removal potential (40–70%) highlights utilization of the bacteria isolated for petrol bioremediation, mineralization of organophosphates, dairy and food industry, and also as biofertilizers and biocontrol agents.</p></abstract>

The present study aimed to isolate the high-efficiency petrol metabolizing thermophilic bacteria from petrol contaminated soil samples. Isolation was carried out through enrichment culture, serial dilution and pour plate methods using the petrol supplemented minimal salt media. The isolated bacteria were analyzed to document growth behavior, petrol removal efficiencies, antibiotic resistance profile, and biochemical characteristics. The 16S rRNA based phylogenetic analysis helped to reveal the identity of isolated bacterial species and construct the phylogenetic trees. Total nine bacteria were isolated, out of which three (IUBP2, IUBP3, IUBP5) were identified as Brevibacillus formosus, one (IUBP1) was found similar to Brevibacillus agri, four (IUBP7, IUBP8, IUBP13, and IUBP14) shared homology with Burkholderia lata, and one (IUBP15) with Burkholderia pyrrocinia. All the isolates were fast growing and exhibited considerable petrol degradation potential. The highest petrol removal efficiency (69.5% ± 13.44/6 days) was recorded for the strain IUBP15 at a petrol concentration of 0.1% (v/v). All bacteria studied (100%) were positive for esculinase and phosphatase. Many strains exhibited positive responses for arginine dehydrolase (22%), β-naphthylamidase (11%), β-D-glucosaminide (33%), mannitol (55%), sorbitol (66%) and inulin (88%) fermentation test. While all were sensitive to the antibiotics, some of them were found resistant against chloramphenicol and oxacillin. The remarkable biochemical characteristics and considerable petrol removal potential (40–70%) highlights utilization of the bacteria isolated for petrol bioremediation, mineralization of organophosphates, dairy and food industry, and also as biofertilizers and biocontrol agents.

An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The present study aimed to isolate the high-efficiency petrol metabolizing thermophilic bacteria from petrol contaminated soil samples. Isolation was carried...

Group	Isolate	Accession No.	Closest phylogenetic relative	Similarity	URLs
Group I	IUBP2	MH023312	Brevibacillus formosus strain NBRC 15716	100%	https://www.ncbi.nlm.nih.gov/nuccore/MH023312.1
	IUBP3	MH023313	Brevibacillus formosus strain NBRC 15716	100%	https://www.ncbi.nlm.nih.gov/nuccore/MH023313.1
	IUBP5	MH023314	Brevibacillus formosus strain NBRC 15716	100%	https://www.ncbi.nlm.nih.gov/nuccore/MH023314.1
Group II	IUBP1	MH368051	Brevibacillus agri strain NBRC 15538	100%	https://www.ncbi.nlm.nih.gov/nuccore/MH368051
Group III	IUBP7	MH368052	Burkholderia lata strain 383	99%	https://www.ncbi.nlm.nih.gov/nuccore/MH368052.1
	IUBP8	MH368053	Burkholderia lata strain 383	99%	https://www.ncbi.nlm.nih.gov/nuccore/MH368053.1
	IUBP13	MH368054	Burkholderia lata strain 383	99%	https://www.ncbi.nlm.nih.gov/nuccore/MH368054.1
	IUBP14	MH368055	Burkholderia lata strain 383	99%	https://www.ncbi.nlm.nih.gov/nuccore/MH368055.1
Group IV	IUBP15	MH368057	Burkholderia pyrrocinia strain LMG 14191	98%	https://www.ncbi.nlm.nih.gov/nuccore/MH368057

An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

Article Category: original-paper

Published Online: Jun 28, 2019

Page range: 193 - 201

Received: Dec 01, 2018

Accepted: Jan 31, 2019

DOI: https://doi.org/10.33073/pjm-2019-019

Keywords
bioremediation, minimal salt media, green technology, gasoline and 16S rRNA profiling

© 2019 Fatima Muccee and Samina Ejaz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Phylogenetic analysis of petrol metabolizing bacteria.

An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

Article Category: original-paper

Published Online: Jun 28, 2019

Page range: 193 - 201

Received: Dec 01, 2018

Accepted: Jan 31, 2019

DOI: https://doi.org/10.33073/pjm-2019-019

Keywordsbioremediation, minimal salt media, green technology, gasoline and 16S rRNA profiling

© 2019 Fatima Muccee and Samina Ejaz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Phylogenetic analysis of petrol metabolizing bacteria.

Keywords
bioremediation, minimal salt media, green technology, gasoline and 16S rRNA profiling