<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>When glucose and Amadori products are auto-oxidized, glycation occurs, resulting in the formation of early (Amadori) and late advanced glycation end products (AGEs), as well as free radicals. Glycation and an increase in free radical activity induce diabetic complications. Antioxidant and antiglycation compounds may aid in the prevention of oxidation and glycation. The goal of this study was to assess the antiglycation and antioxidant capacity of C-phycocyanin (C-PC) derived from Plectonema sp. The DPPH (1, 1-diphenyl-2-picrylhydrazyl), nitric oxide, hydroxyl radical scavenging activities and ferric ions reducing antioxidant power (FRAP) assays were used to assess antioxidant activity, while an in vitro bovine serum albumin-methyl glyoxal glycation (BSA-MG) model was used to assess glycation inhibitory potential. Glycation inhibition was measured using a variety of spectroscopic and biochemical parameters, including UV-visible &amp; fluorescence spectroscopy, ketoamine, carbonyl and hydroxymethyl furfural content, as well as free lysine &amp; free arginine estimations. In vitro, C-PC exhibited dose-dependent potent antioxidant activity, but lacked significant antiglycation potential. As a result, it is recommended that further studies be conducted to evaluate the antiglycation potential of C-PC.</p>
</abstract>

When glucose and Amadori products are auto-oxidized, glycation occurs, resulting in the formation of early (Amadori) and late advanced glycation end products (AGEs), as well as free radicals. Glycation and an increase in free radical activity induce diabetic complications. Antioxidant and antiglycation compounds may aid in the prevention of oxidation and glycation. The goal of this study was to assess the antiglycation and antioxidant capacity of C-phycocyanin (C-PC) derived from Plectonema sp. The DPPH (1, 1-diphenyl-2-picrylhydrazyl), nitric oxide, hydroxyl radical scavenging activities and ferric ions reducing antioxidant power (FRAP) assays were used to assess antioxidant activity, while an in vitro bovine serum albumin-methyl glyoxal glycation (BSA-MG) model was used to assess glycation inhibitory potential. Glycation inhibition was measured using a variety of spectroscopic and biochemical parameters, including UV-visible & fluorescence spectroscopy, ketoamine, carbonyl and hydroxymethyl furfural content, as well as free lysine & free arginine estimations. In vitro, C-PC exhibited dose-dependent potent antioxidant activity, but lacked significant antiglycation potential. As a result, it is recommended that further studies be conducted to evaluate the antiglycation potential of C-PC.

Non-inhibitory effects of the potent antioxidant <italic>C-phycocyanin</italic> from <italic>Plectonema</italic> sp. on the <italic>in vitro</italic> glycation reaction

Department of Medical Laboratory Sciences

Department of Basic Dental and Medical Sciences

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution 4.0 International License.

Non-inhibitory effects of the potent antioxidant  from  sp. on the  glycation reaction

When glucose and Amadori products are auto-oxidized, glycation occurs, resulting in the formation of early (Amadori) and late advanced glycation end products...

Non-inhibitory effects of the potent antioxidant C-phycocyanin from Plectonema sp. on the in vitro glycation reaction

Published Online: May 09, 2022

Page range: 199 - 213

Received: Nov 25, 2021

Accepted: Apr 08, 2022

DOI: https://doi.org/10.2478/rrlm-2022-0018

Keywords
glycation, antioxidant, antiglycation, plectonema sp., C-phycocyanin, Bovine Serum Albumin (BSA), Methylglyoxal (MG)

© 2022 Arbab Husain et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Non-inhibitory effects of the potent antioxidant C-phycocyanin from Plectonema sp. on the in vitro glycation reaction

Published Online: May 09, 2022

Page range: 199 - 213

Received: Nov 25, 2021

Accepted: Apr 08, 2022

DOI: https://doi.org/10.2478/rrlm-2022-0018

Keywordsglycation, antioxidant, antiglycation, plectonema sp., C-phycocyanin, Bovine Serum Albumin (BSA), Methylglyoxal (MG)

© 2022 Arbab Husain et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
glycation, antioxidant, antiglycation, plectonema sp., C-phycocyanin, Bovine Serum Albumin (BSA), Methylglyoxal (MG)