<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec><h3>Introduction</h3>
<p> Venous thromboembolism (VTE) is the third most common hemostatic disease worldwide. Studies have reported a role for microRNA (miRNA) in the homeostasis and development of VTE. The ras-related nuclear protein (<italic>RAN</italic>) and exportin 5 (<italic>XPO5</italic>) genes are involved in miRNA biogenesis, as both regulate the transport of pre-miRNA from the nucleus to the cytoplasm. Therefore, the aim of the current study is to examine the association between <italic>RAN</italic> (rs14035) and <italic>XPO5</italic> (rs11077) single nucleotide polymorphisms (SNPs) and VTE.</p>
</sec>
<sec><h3>Methods</h3>
<p> The study sample consisted of 300 subjects (150 patients and 150 age and sex matched controls). The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and tetra‐primer amplification refractory mutation system (T-ARMS) techniques were used to genotype rs14035 and rs11077, respectively.</p>
</sec>
<sec><h3>Results</h3>
<p> The results showed that there was a significant association between the <italic>XPO5</italic> rs11077 and the risk of VTE (P &lt; 0.05). Subjects with AC (OR: 2.08, CI:1.26–3.44) and CC (OR: 1.77, CI: 0.88–3.55) genotypes were at increased risk of the developing VTE. Regarding <italic>RAN</italic> gene, no association was found between rs14035 and VTE (P > 0.05). In addition, no associations were found between <italic>XPO5</italic> rs11077 and <italic>RAN</italic> rs14035 genotypes with blood cell parameters (P > 0.05). As for the demographic characteristics, the results indicated a strong association between family history and body mass index (BMI) with the risk of VTE (P &lt; 0.01).</p>
</sec>
<sec><h3>Conclusion</h3>
<p> The <italic>XPO5</italic> rs11077, BMI and family history might contribute to the development of VTE in Jordan.</p>
</sec>
</abstract>

Introduction
 Venous thromboembolism (VTE) is the third most common hemostatic disease worldwide. Studies have reported a role for microRNA (miRNA) in the homeostasis and development of VTE. The ras-related nuclear protein (RAN) and exportin 5 (XPO5) genes are involved in miRNA biogenesis, as both regulate the transport of pre-miRNA from the nucleus to the cytoplasm. Therefore, the aim of the current study is to examine the association between RAN (rs14035) and XPO5 (rs11077) single nucleotide polymorphisms (SNPs) and VTE.

Methods
 The study sample consisted of 300 subjects (150 patients and 150 age and sex matched controls). The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and tetra‐primer amplification refractory mutation system (T-ARMS) techniques were used to genotype rs14035 and rs11077, respectively.

Results
 The results showed that there was a significant association between the XPO5 rs11077 and the risk of VTE (P < 0.05). Subjects with AC (OR: 2.08, CI:1.26–3.44) and CC (OR: 1.77, CI: 0.88–3.55) genotypes were at increased risk of the developing VTE. Regarding RAN gene, no association was found between rs14035 and VTE (P > 0.05). In addition, no associations were found between XPO5 rs11077 and RAN rs14035 genotypes with blood cell parameters (P > 0.05). As for the demographic characteristics, the results indicated a strong association between family history and body mass index (BMI) with the risk of VTE (P < 0.01).

Conclusion
 The XPO5 rs11077, BMI and family history might contribute to the development of VTE in Jordan.

Investigation of the association of the <italic>RAN</italic> (rs14035) and <italic>XPO5</italic> (rs11077) polymorphisms with venous thromboembolism

Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences

Jordan University of Science and Technology

Romanian Journal of Internal Medicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Investigation of the association of the  (rs14035) and  (rs11077) polymorphisms with venous thromboembolism

Introduction
 Venous thromboembolism (VTE) is the third most common hemostatic disease worldwide. Studies have reported a role for microRNA (miRNA) in the homeostasis and development of VTE. The ras-related nuclear protein (RAN) and exportin 5 (XPO5) genes are involved in miRNA biogenesis, as both regulate the transport of pre-miRNA from the nucleus to the cytoplasm. Therefore, the aim of the current study is to examine the association between RAN (rs14035) and XPO5 (rs11077) single nucleotide polymorphisms (SNPs) and VTE.

Methods
 The study sample consisted of 300 subjects (150 patients and 150 age and sex matched controls). The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and tetra‐primer amplification refractory mutation system (T-ARMS) techniques were used to genotype rs14035 and rs11077, respectively.

Results
 The results showed that there was a significant association between the XPO5 rs11077 and the risk of VTE (P  0.05). In addition, no associations were found between XPO5 rs11077 and RAN rs14035 genotypes with blood cell parameters (P > 0.05). As for the demographic characteristics, the results indicated a strong association between family history and body mass index (BMI) with the risk of VTE (P < 0.01).

Conclusion
 The XPO5 rs11077, BMI and family history might contribute to the development of VTE in Jordan.

Introduction
 Venous thromboembolism (VTE) is the third most common hemostatic disease worldwide. Studies have reported a role for microRNA (miRNA) in the...

Investigation of the association of the RAN (rs14035) and XPO5 (rs11077) polymorphisms with venous thromboembolism

Published Online: Jun 13, 2023

Page range: 154 - 162

Received: May 06, 2023

DOI: https://doi.org/10.2478/rjim-2023-0014

Keywords
MicroRNAs, Venous Thromboembolism, Risk, Genes, Single nucleotide polymorphism

© 2023 Khloud M. Alquraan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Investigation of the association of the RAN (rs14035) and XPO5 (rs11077) polymorphisms with venous thromboembolism

Published Online: Jun 13, 2023

Page range: 154 - 162

Received: May 06, 2023

DOI: https://doi.org/10.2478/rjim-2023-0014

KeywordsMicroRNAs, Venous Thromboembolism, Risk, Genes, Single nucleotide polymorphism

© 2023 Khloud M. Alquraan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
MicroRNAs, Venous Thromboembolism, Risk, Genes, Single nucleotide polymorphism