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Correlation of t(14;18) translocation breakpoint site with clinical characteristics in follicular lymphoma

Matej Panjan
Matej Panjan
, 
Lucka Boltezar
Lucka Boltezar
, 
Srdjan Novakovic
Srdjan Novakovic
, 
Ira Kokovic
Ira Kokovic
 and 
Barbara Jezersek Novakovic
Barbara Jezersek Novakovic
   | Jul 13, 2023
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Article Category: Research Article
Published Online: Jul 13, 2023
Page range: 487 - 492
Received: Apr 04, 2023
Accepted: May 31, 2023
DOI: https://doi.org/10.2478/raon-2023-0030
Keywords
© 2023 Matej Panjan et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

FIGURE 1.

Diagram of the BCL2/JH t(14;18) translocation breakpoints. Relative positions of major breakpoint region (MBR), 3′MBR subcluster, 5′mcr subcluster and minor cluster region (mcr) are shown according to the report of van Dongen JJM et al.16
Diagram of the BCL2/JH t(14;18) translocation breakpoints. Relative positions of major breakpoint region (MBR), 3′MBR subcluster, 5′mcr subcluster and minor cluster region (mcr) are shown according to the report of van Dongen JJM et al.16

FIGURE 2.

Comparison of progression-free survival between the 3′MBR (blue), MBR (red) and mcr (green) groups. Censored cases are marked as vertical lines on their respective curves. Log Rank (Mantel-Cox) significance: 0.157. Log Rank (Mantel-Cox) significance comparing the MBR and mcr group: 0.235.MBR = major breakpoint region; mcr = minor cluster region
Comparison of progression-free survival between the 3′MBR (blue), MBR (red) and mcr (green) groups. Censored cases are marked as vertical lines on their respective curves. Log Rank (Mantel-Cox) significance: 0.157. Log Rank (Mantel-Cox) significance comparing the MBR and mcr group: 0.235.MBR = major breakpoint region; mcr = minor cluster region

FIGURE 3.

Comparison of overall survival between the 3′MBR (blue), MBR (red) and mcr (green) groups. Censored cases are marked as vertical lines on their respective curves. Log Rank (Mantel-Cox) significance: 0.426. Log Rank (Mantel-Cox) significance comparing the MBR and mcr group: 0.351.MBR = major breakpoint region; mcr = minor cluster region
Comparison of overall survival between the 3′MBR (blue), MBR (red) and mcr (green) groups. Censored cases are marked as vertical lines on their respective curves. Log Rank (Mantel-Cox) significance: 0.426. Log Rank (Mantel-Cox) significance comparing the MBR and mcr group: 0.351.MBR = major breakpoint region; mcr = minor cluster region

FIGURE 4.

Comparison of lymphoma-specific overall survival between the 3′MBR (blue), MBR (red) and mcr (green) groups. Censored cases are marked as vertical lines on their respective curves. Log Rank (Mantel-Cox) significance: 0.409. Log Rank (Mantel-Cox) significance comparing the MBR and mcr groups: 0.301.MBR = major breakpoint region, mcr = minor cluster region
Comparison of lymphoma-specific overall survival between the 3′MBR (blue), MBR (red) and mcr (green) groups. Censored cases are marked as vertical lines on their respective curves. Log Rank (Mantel-Cox) significance: 0.409. Log Rank (Mantel-Cox) significance comparing the MBR and mcr groups: 0.301.MBR = major breakpoint region, mcr = minor cluster region

Comparison of clinical features at diagnosis between the breakpoint-site groups (major breakpoint region [MBR], 3′MBR, mcr)

MBR (N = 63) 3′MBR (N = 4) mcr (N = 17) p1 p2
Median (mean) stage 4 (3.70) 4 (3.75) 4 (3.94) 0.361 0.023
Median (mean) FLIPI 2 (2.51) 3 (2.75) 3 (3.00) 0.226 0.094
Median (mean) age 61 (60.25) 62 (63.25) 64 (63.71) 0.423 0.218

Sequences of primers used for detection of the t(14;18)(q32;q21) translocation. Relative positions of primers are indicated downstream of the first nucleotide of corresponding reference sequence

t(14;18) MBR primers
primer name NCBI accession no. position primer sequence
MBR1 AY220759.1 (+193443) 5′-GACCAGCAGATTCAAATCTATGG-3′
MBR2 AY220759.1 (+192940) 5′-ACTCTGTGGCATTATTGCATTATAT-3′
t(14;18) 3′MBR primers
primer name NCBI accession no. position primer sequence
3′MBR1 AH010747.2 (+717) 5′-GCACCTGCTGGATACAACACTG-3′
3′MBR2 AH010747.2 (+1530) 5′-GGTGACAGAGCAAAACATGAACA-3′
3′MBR3 AH010747.2 (+1787) 5′-GTAATGACTGGGGAGCAAATCTT-3′
3′MBR4 AH010747.2 (+2718) 5′-ACTGGTTGGCGTGGTTTAGAGA-3′
t(14;18) mcr primers
primer name NCBI accession no. position primer sequence
mcr1 AF275873.1 (+1961) 5′-TAGAGCAAGCGCCCAATAAATA-3′
mcr2 AF275873.1 (+2407) 5′-TGAATGCCATCTCAAATCCAA-3′
5′mcr AH010747.2 (+15849) 5′-CCTTCTGAAAGAAACGAAAGCA-3′
Consensus JH primer
primer name NCBI accession no. position primer sequence
JH OL807663.1 (+239) 3′-CCAGTGGCAGAGGAGTCCATTC-5′

Comparison of clinical features at diagnosis between the breakpoint-site groups (MBR, 3′MBR, mcr) using Fisher's exact test

MBR (N = 63) 3′MBR (N = 4) mcr (N = 17) p1 p2
Male sex 24 (38%) 1 (25%) 4 (24%) 0.571 0.391
Grade* 3 11 (20%) 2 (50%) 4 (25%) 0.303 0.729
B-symptoms 23 (37%) 2 (50%) 8 (47%) 0.641 0.576
Bulky disease** 17 (27%) 1 (25%) 2 (12%) 0.497 0.335
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