Correlation of t(14;18) translocation breakpoint site with clinical characteristics in follicular lymphoma

FIGURE 1.

FIGURE 2.

FIGURE 3.

FIGURE 4.

Comparison of clinical features at diagnosis between the breakpoint-site groups (major breakpoint region [MBR], 3′MBR, mcr)

	MBR (N = 63)	3′MBR (N = 4)	mcr (N = 17)	p1	p2
Median (mean) stage	4 (3.70)	4 (3.75)	4 (3.94)	0.361	0.023
Median (mean) FLIPI	2 (2.51)	3 (2.75)	3 (3.00)	0.226	0.094
Median (mean) age	61 (60.25)	62 (63.25)	64 (63.71)	0.423	0.218

Sequences of primers used for detection of the t(14;18)(q32;q21) translocation_ Relative positions of primers are indicated downstream of the first nucleotide of corresponding reference sequence

t(14;18) MBR primers
primer name	NCBI accession no.	position	primer sequence
MBR1	AY220759.1	(+193443)	5′-GACCAGCAGATTCAAATCTATGG-3′
MBR2	AY220759.1	(+192940)	5′-ACTCTGTGGCATTATTGCATTATAT-3′
t(14;18) 3′MBR primers
primer name	NCBI accession no.	position	primer sequence
3′MBR1	AH010747.2	(+717)	5′-GCACCTGCTGGATACAACACTG-3′
3′MBR2	AH010747.2	(+1530)	5′-GGTGACAGAGCAAAACATGAACA-3′
3′MBR3	AH010747.2	(+1787)	5′-GTAATGACTGGGGAGCAAATCTT-3′
3′MBR4	AH010747.2	(+2718)	5′-ACTGGTTGGCGTGGTTTAGAGA-3′
t(14;18) mcr primers
primer name	NCBI accession no.	position	primer sequence
mcr1	AF275873.1	(+1961)	5′-TAGAGCAAGCGCCCAATAAATA-3′
mcr2	AF275873.1	(+2407)	5′-TGAATGCCATCTCAAATCCAA-3′
5′mcr	AH010747.2	(+15849)	5′-CCTTCTGAAAGAAACGAAAGCA-3′
Consensus JH primer
primer name	NCBI accession no.	position	primer sequence
JH	OL807663.1	(+239)	3′-CCAGTGGCAGAGGAGTCCATTC-5′

Comparison of clinical features at diagnosis between the breakpoint-site groups (MBR, 3′MBR, mcr) using Fisher's exact test

	MBR (N = 63)	3′MBR (N = 4)	mcr (N = 17)	p1	p2
Male sex	24 (38%)	1 (25%)	4 (24%)	0.571	0.391
Grade* 3	11 (20%)	2 (50%)	4 (25%)	0.303	0.729
B-symptoms	23 (37%)	2 (50%)	8 (47%)	0.641	0.576
Bulky disease**	17 (27%)	1 (25%)	2 (12%)	0.497	0.335