Endometrial carcinoma (EC) is a common malignant tumor of the female reproductive system worldwide, and approximately 80% of newly diagnosed EC patients are in the early stage (International Federation of Gynecology and Obstetrics (FIGO) stage IA, IB).1 The TP53 is an important suppressor gene that is deeply involved in tumorigenesis and can control cell growth, apoptosis and regulate angiogenesis. Several studies have shown that high expression of TP53 is closely associated with poor prognosis in EC patients.2,3 Risk stratification based on the histologic subtype, grade, FIGO stage, and lymphovascular space invasion (LVSI) is the primary basis for determining treatment strategies for early-stage EC.4 For non-low-risk (intermediate-, high-intermediate-, and high-risk) patients, lymphadenectomy (LND) is required in addition to the standard treatment of total hysterectomy with bilateral salpingo-oophorectomy, since it can significantly improve patient benefit. But for low-risk patients, LND is not recommended as it is likely to lead to complications and increased care costs.5 Currently, preoperative biopsy and routine magnetic resonance imaging (MRI) are the primary means of obtaining the TP53 status and risk stratification information of EC, respectively.6 However, biopsy may not be sufficient for a reliable diagnosis due to shortcomings such as unstable sampling depending on operator experience, inadequate sampling, and invasiveness.7,8 At the same time, conventional T1-weighted imaging (T1WI) and T2-weighted imaging (T2WI) not only fail to reflect the TP53 status, histological subtype, and grade information of the lesion but also likely to have a poor to moderate pooled sensitivity in detecting high-risk factors, including deep myometrial invasion and cervical stromal infiltration, due to the presence of adenomyosis and leiomyomas and the loss of the junctional zone.9,10 Therefore, finding a noninvasive and effective means to assess the TP53 status and risk stratification in early-stage EC is of great benefit to patients.
Dynamic contrast-enhanced MRI (DCE-MRI) is a promising quantitative MRI sequence that can detect blood supply in biological tissues by analyzing the dynamic distribution of contrast agents through pharmacokinetic models.11,12 Intravoxel incoherent motion (IVIM) can also be used to reflect blood perfusion, and compared to DCE-MRI, it not only eliminates the need for contrast agents but also provides additional information on the diffusion of water molecules within the lesion.13,14,15,16 Recently, some authors have used IVIM and DCE-MRI for EC-related studies. For example, Satta
The purpose of this study was to investigate the contributory value of quantitative parameters derived from DCE-MRI and IVIM in differentiating TP53-mutant from TP53-wild, low-risk from non-low-risk early-stage EC, offering a potential reference for the clinical management of early-stage EC.
This prospective study was complied with ethical committee standards and approved by the ethics committee of the First Affiliated Hospital of Xinxiang Medical University (NO. EC-022-002) and informed consent was taken from all individual participants. From January 2021 to April 2022, 114 female patients underwent pelvic MRI due to suspected EC by clinical examination, ultrasound (US), or computed tomography (CT). Forty participants were excluded during this study: 1) 7 patients were diagnosed with an endometrial polyp, atypical hyperplasia, or other non-EC diseases; 2) 16 patients had FIGO stage ≥ II; 3) 4 patients received radiotherapy or neoadjuvant chemotherapy; 4) 3 patients had claustrophobia or other diseases that prevented them from completing all the sequences; 5) 6 patients had inadequate DCE-MRI or IVIM imaging quality for analysis due to severe artifacts, and 6) 4 patients decided to perform histological analysis and treatment in other institutes. Ultimately, 74 patients were enrolled in the study (Figure 1).
A 1.5 T MR system (Optima MR360, Waukesha, WI, USA) with a 12-channel phased-array body coil was used in this study. The imaging protocol included oblique axial (perpendicular to the long axis of the uterus) T1WI, T2WI, DWI, IVIM, and DCE-MRI. For DWI and DCE-MRI sequences, the scans covered the anterior superior iliac spine to the symphysis pubis. For IVIM (b = 0, 20, 40, 80, 160, 200, 400, 600, 800, and 1000 s/mm2), to minimize scan time, the scan was limited to the lesion area (determined by an experienced radiologist from the DWI images), and its location, layer thickness, and layer spacing were consistent with the corresponding layer of DWI.18 DCE-MRI was performed by a three-dimensional liver acquisition with volume acceleration (3D-LAVA) sequence with 40 phases (time resolution, 9s), and gadopentetate dimeglumine (Gd-DTPA, Bayer Pharmaceutical, Berlin, Germany) was injected intravenously with an automatic injector (0.2 mL/kg, 3.0 mL/s). The protocol details are provided in Table 1.
Imaging protocol parameters
Sequence | 2D-FSE | 2D-FSE | 2D-SS-EPI | 2D-SS-EPI | 3D-LAVA |
Orientation | Oblique Axial | Oblique Axial | Oblique Axial | Oblique Axial | Oblique Axial |
TR/TE (ms) | 659/12.3 | 6000/95 | 3708/74.3 | 2000/80.7 | 3.5/1.7 |
FOV (cm2) | 40 × 40 | 40 × 40 | 40 × 40 | 40 × 40 | 36 × 36 |
Matrix | 288 × 192 | 320 × 320 | 96 × 128 | 128 × 192 | 288 × 192 |
Flip angle (°) | 160 | 160 | 90 | 90 | 15 |
Slice thickness (mm) | 6 | 6 | 6 | 6 | 6 |
No. of sections | 20 | 20 | 20 | Based on lesion's size | 26 |
NEX | 1 | 1 | 1, 4 | 1, 1, 1, 1, 1, 1, 2, 4, 4, 6 | 0.73 |
Fat suppression | / | STIR | STIR | STIR | FLEX |
b-values (s/mm2) | / | / | 0, 800 | 0, 20, 40, 80, 160, 200, 400, 600, 800, 1000 | / |
Respiratory compensation | Free | Free | Free | Free | Free |
Scan time | 1 min 56 s | 48 s | 1 min 04 s | 3~6min | 6 min 08 s (40 phases) |
DCE-MRI = dynamic contrast-enhanced magnetic resonance imaging; DWI = diffusion-weighted imaging; FOV = field of view; FLEX = FLEXible; FSE = fast spin echo; IVIM = intravoxel incoherent motion; LAVA = liver acquistion with volume assessmeNT; NEX = number of excitations; SS-EPI = single shot echo planar imaging; STIR = short-inversion time(TI) recovery; TR/TE = repetition time/echo time; T1WI = T1-weighted imaging; T2WI = T2-weighted imaging
All images were transferred to the Advantage Workstation (version 4.7), and the IVIM and DCE-MRI images were analyzed within the workstation using vendor-provided software named MADC and GenIQ, respectively. The IVIM parameters were calculated by the following formula:
For regions of interest (ROI), first, images of DCE-MRI and IVIM were co-registered, and then on the DCE-MRI images of the phase with the clearest lesion display21, ROIs were delineated layer by layer for all slices containing the tumor, and these ROIs were manually drawn along the inside margin of the primary tumor, avoiding areas with cystic degeneration, necrosis, apparent signs and hemorrhage artifacts, and blood vessels. Subsequently, all completed ROIs were automatically copied to the pseudo-color maps of the DCE-MRI and IVIM-derived parameters to calculate the mean values based on the volume of interest (VOI). All of these procedures were completed independently by two radiologists with 7 and 15 years of experience who were blinded to each other's results and the patient's clinicopathological data.
All lesion specimens were obtained surgically, and the median interval from pelvic MRI examination to surgery was 12 days (1–25 days). The specimens were processed by an experienced pathologist. The histological subtype, grade, and LVSI were confirmed by hematoxylin/eosin (HE) staining. The stage was estimated with the FIGO staging system.22 According to the European Society for Medical Oncology (ESMO) clinical practice guidelines, low-risk patients were classified into the low-risk group, while intermediate-risk, high-intermediate-risk, and high-risk patients were classified into the non-low-risk group.4 The TP53 status was evaluated by immunohistochemical (IHC) staining, where non-staining was viewed as the wild group, and faint, moderate, and strong staining was viewed as the mutant group. Ultimately, risk stratification was evaluated in all 74 patients, and TP53 status was evaluated in 46 patients (28 patients declined IHC for financial or other reasons).
All data were analyzed with Stata version 16.0 (Stata Corp) and MedCalc version 15.0 (MedCalc Software). P < 0.05 was considered statistically significant. The interobserver consistency of two radiologists was classified using the intraclass correlation coefficient (ICC) as poor (ICC < 0.40), fair (0.40 ≤ ICC < 0.60), good (0.60 ≤ r < 0.75), or excellent (ICC ≥ 0.75).23 The Shapiro–Wilk test was employed to check the normality of the data. The Mann–Whitney U test and the independent samples t-test were used for nonnormally distributed data (median and interquartile range) and normally distributed data (mean ± standard deviation), respectively. The area under the receiver operating characteristic (ROC) curve (AUC) was employed to quantify the diagnostic efficacy of different parameters, and the differences were assessed using DeLong analysis. The combination of parameters was investigated by logistic regression, evaluated by bootstrap (random number set 123, repeated sampling 1000 times, backward strategy, bounded by a value of 0.1), calibration curves, and decision curve analysis (DCA).24
The clinicopathological and imaging characteristics are shown in Table 2 and Figure 2, respectively.
Clinicopathologic features of the patients
Age (mean ± SD) (years) | 54.00 ± 7.91 |
Maximum diameter (mean ± SD) (mm) | 25.10 (13.76, 42.58) |
FIGO stage n (%) | |
IA | 44 (59.46) |
IB | 30 (40.54) |
Histologic subtype n (%) | |
Adenocarcinoma | 67 (90.54) |
Non-adenocarcinoma | 7 (9.46) |
Clear-cell | 3 (4.06) |
Undifferentiated carcinoma | 2 (2.70) |
Carcinosarcoma | 2 (2.70) |
Lymphovascular space invasion n (%) | |
Positive | 10 (6.76) |
Negative | 64 (93.24) |
Histologic grade n (%) | |
Grade 1 | 54 (72.98) |
Grade 2 | 10 (13.51) |
Grade 3 | 10 (13.51) |
Risk stratification n (%) | |
Low | 44 (59.46) |
Intermediate | 20 (27.03) |
High-intermediate | 0 (0.00) |
High | 10 (13.51) |
TP53 expression | |
Mutant | 21 (28.38) |
Wild | 25 (33.78) |
No result | 28 (37.84) |
FIGO = International Federation of Gynecology and Obstetrics; SD = standard deviation
The D, D*, f, Ktrans, Ve, and Kep measured by 2 radiologists had excellent consistency, and the ICCs were 0.864 (95% CI: 0.788 – 0.913), 0.799 (95% CI: 0.696 – 0.867), 0.855 (95% CI: 0.729 – 0.918), 0.868 (95% CI: 0.799 – 0.915), 0.834 (95% CI: 0.748 – 0.892), and 0.828 (95% CI: 0.739 – 0.888), respectively. The average results were used for the ultimate analysis.
The Ktrans and Kep were higher and D was lower in the TP53-mutant group than in the TP53-wild group (P = 0.038, 0.002, and 0.037, respectively), f, D*, and Ve were not significantly different between the two groups (P = 0.750, 0.604, and 0.434, respectively). The Ktrans, Ve, f, and D values were lower in the non-low-risk group than in the low-risk group (P < 0.001, < 0.001, 0.002, and < 0.001, respectively), Kep and D* were not significantly different between the two groups (P = 0.218 and 0.601) (Table 3, Figure 3).
Comparison of different parameters
Risk stratification | ||||||
High-risk (n = 10) | 0.63 (0.40, 0.73) | 58.40 (40.10, 88.73) | 1.64 ± 0.60 | 0.35 (0.15, 0.43) | 0.30 ± 0.07 | 1.23 (0.48, 1.54) |
High-intermediate-risk (n = 0) | / | / | / | / | / | / |
Intermediate-risk (n = 20) | 0.55 (0.40, 0.81) | 52.00 (26.88, 74.33) | 1.74 ± 0.96 | 0.37 (0.29, 0.47) | 0.33 ± 0.14 | 1.24 (0.82, 1.98) |
Low-risk (n = 44) | 0.86 (0.64, 1.16) | 44.35 (21.93, 95.33) | 2.43 ± 1.08 | 0.61 (0.43, 1.14) | 0.58 ± 0.25 | 1.53 (0.79, 2.21) |
P-value | 0.464 a | 0.191 a | ||||
P-value (High |
0.880 b | 0.248 b | 0.735 c | 0.397 b | 0.532 c | 0.307 b |
P-value (High |
0.238 b | 0.099 b | ||||
P-value (Intermediate vs Low) | 0.937 b | 0.582 | ||||
Low-risk (n = 44) | 0.86 (0.64, 1.16) | 44.35 (21.93, 95.33) | 2.43 ± 1.08 | 0.61 (0.43, 1.14) | 0.58 ± 0.25 | 1.53 (0.79, 2.21) |
Non-low-risk (High + Intermediate, n = 30) | 0.58 (0.40, 0.77) | 55.25 (34.63, 72.78) | 1.71 ± 0.84 | 0.37 (0.28, 0.45) | 0.32 ± 0.12 | 1.23 (0.82, 1.87) |
z/t value | − 3.793 | −0.523 | 3.234 | −5.109 | 5.304 | −1.233 |
P-value | 0.601 b | 0.218 b | ||||
TP53 expression | ||||||
Mutant (n = 21) | 0.72 ± 0.31 | 43.70 (16.30, 90.75) | 2.30 ± 1.09 | 0.67 (0.41, 1.14) | 0.32 (0.25, 0.91) | 1.67 (1.17, 2.09) |
Wild (n = 25) | 0.91 ± 0.29 | 50.60 (26.90, 82.75) | 2.20 ± 1.02 | 0.43 (0.37, 0.49) | 0.49 (0.36, 0.76) | 0.90 (0.58, 1.55) |
Z/t value | −2.155 | −0.518 | 0.321 | −2.073 | −0.783 | −3.165 |
P-value | 0.604 b | 0.750 c | 0.434 b |
The bold typeface in the table indicates the comparison with statistical significance.
Comparisons were performed by
comparisons were performed by Mann–Whitney U test;
comparisons were performed by independent t test.
In the identification of TP53-mutant and TP53-wild early-stage EC, the potential related factors such as age, tumor size, risk stratification, FIGO stage, subtype, grade, LVSI, D, D*, f, Ktrans, Ve, and Kep were all enrolled in regression analysis. Univariate analysis demonstrated that grade, D, Ktrans, and Kep were all risk predictors (P all < 0.1), while multivariate analysis showed that only D and Ktrans were independent predictors (P = 0.003, 0.016).
In the identification of non-low-risk and low-risk early-stage EC, potential risk-related factors such as age, tumor size, TP53 status, D, D*, f, Ktrans, Ve, and Kep were all enrolled in regression analysis. Univariate analysis demonstrated that tumor size, D, f, Ktrans, and Ve were all risk predictors (P all < 0.1), while multivariate analysis showed that only f, Ktrans, and Ve were independent predictors (P = 0.036, 0.003, and 0.024, respectively) (Table 4).
Logistic regression analyses
Low vs non-low risk | ||||
Age (year) | 1.462 (0.894–2.388) | 0.130 | / | / |
Tumor size (mm) | 1.055 (1.003–1.110) | 1.083 (0.979–1.197) | 0.123 | |
TP53 mutant | 1.506 (0.407–5.578) | 0.540 | / | / |
D (×10−3mm2/s) | 0.089 (0.021–0.373) | 0.144 (0.015–1.334) | 0.088 | |
D* (×10−3mm2/s) | 0.867 (0.533–1.412) | 0.567 | / | / |
f (%) | 0.419 (0.226–0.776) | 0.292 (0.093–0.921) | ||
Ktrans (min−1) | 0.009 (0.001–0.153) | 0.001 (0.000–0.089) | ||
Ve | 0.173 (0.069–0.432) | 0.130 (0.022–0.766) | ||
Kep (min−1) | 0.642 (0.367–1.126) | 0.122 | / | / |
TP53 mutant vs wild | ||||
Age (year) | 0.855 (0.465–1.548) | 0.605 | / | / |
Tumor size (mm) | 1.175 (0.649–2.127) | 0.594 | / | / |
Subtype | 77.708 (0.001–100.5) | 0.999 | / | / |
Grade | 2.099 (0.957–4.602) | 1.961 (0.816–4.717) | 0.132 | |
Risk stratification | 1.506 (0.407–5.578) | 0.540 | / | / |
FIGO stage | 1.360 (0.739–2.505) | 0.323 | / | / |
LVSI | 802.578 (0.001–1150.5) | 0.999 | / | / |
D (×10−3mm2/s) | 2.063 (1.016–4.191) | 8.274 (2.066–33.136) | ||
D* (×10−3mm2/s) | 1.020 (0.567–1.835) | 0.948 | / | / |
f (%) | 0.906 (0.504–1.629) | 0.742 | / | / |
Ktrans (min−1) | 0.487 (0.236–1.003) | 0.155 (0.034–0.710) | ||
Ve | 1.008 (0.560–1.812) | 0.979 | / | / |
Kep (min−1) | 0.501 (0.244–1.032) | 1.172 (0.425–3.234) | 0.759 |
D = true diffusion coefficient; D* = pseudo-diffusion coefficient; f = microvascular volume fraction; FIGO = international federation of gynecology and obstetrics; CI = confidence interval; Kep = rate transfer constant; Ktrans = volume transfer constant; LVSI = lymphovascular space invasion; OR = odds ratio;. SD = standard deviation; Ve = volume of extravascular extracellular space per unit volume of tissue
The bold typeface in the table indicates the logistic regression analyses with statistical significance.
In the analysis of the high- and low-risk group, the TP53 mutant data were analysed only for these patients who had the p53 gene test. The remaining parameters, such as diameter, were analysed for all 74 patients.
In the differentiation of TP53-mutant and TP53-wild early-stage EC, the combination of independent predictors (Ktrans and D) showed the optimal diagnostic efficacy (AUC = 0.867; sensitivity, 92.00%; specificity, 80.95%; P < 0.001), which was significantly better than D (AUC = 0.694, Z = 2.169, P = 0.030), and Ktrans (AUC = 0.679, Z = 2.572, P = 0.010). However, the difference between the combination of independent predictors and Kep (AUC = 0.773) was not significant (AUC = 0.773, Z = 1.272, P = 0.203) (Figure 4A, Table 5).
Predictive performance of different parameters
Low vs non-low risk | ||||||
D (×10−3mm2/s) | 0.761 (0.648–0.853) | < 0.001 | 0.691 | 73.33% | 72.73% | Z = 3.113, P = 0.002 |
D* (×10−3mm2/s) | 0.536 (0.416–0.653) | 0.598 | / | / | / | / |
f (%) | 0.688 (0.569–0.790) | 0.003 | 1.240 | 36.67% | 93.18% | Z = 4.317, P < 0.001 |
Ktrans (min−1) | 0.852 (0.750–0.924) | < 0.001 | 0.487 | 90.00% | 68.18% | Z = 2.713, P = 0.007 |
Ve | 0.808 (0.700–0.890) | < 0.001 | 0.401 | 83.33% | 70.45% | Z = 3.175, P = 0.002 |
Kep (min−1) | 0.585 (0.652–0.849) | 0.204 | / | / | / | / |
Combined diagnosis 1 | 0.947 (0.869–0.986) | < 0.001 | / | 83.33% | 93.18% | / |
TP53 mutant vs wild | ||||||
D (×10−3mm2/s) | 0.694 (0.541–0.821) | 0.019 | 0.605 | 92.00% | 47.62% | Z = 2.169, P = 0.030 |
D* (×10−3mm2/s) | 0.545 (0.391–0.692) | 0.498 | / | / | / | / |
f (%) | 0.535 (0.382–0.648) | 0.388 | / | / | / | / |
Ktrans (min−1) | 0.679 (0.525–0.809) | 0.036 | 0.499 | 80.00% | 61.90% | Z = 2.572, P = 0.010 |
Ve | 0.568 (0.413–0.713) | 0.675 | / | / | / | / |
Kep (min−1) | 0.773 (0.626–0.884) | < 0.001 | 1.557 | 80.00% | 66.67% | Z = 1.272, P = 0.203 |
Combined diagnosis 2 | 0.867 (0.734–0.949) | < 0.001 | / | 92.00% | 80.95% | / |
AUC =
The combined diagnosis 1 represents f + Ktrans + Ve; the combined diagnosis 2 represents D + Ve
In the differentiation of low-risk and non-low-risk early-stage EC, the combination of independent predictors (f, Ktrans, and Ve) showed the optimal diagnostic efficacy (AUC = 0.947; sensitivity, 83.33%; specificity, 93.18%; P < 0.001), which was significantly better than D (AUC = 0.761, Z = 3.113, P = 0.002), f (AUC = 0.688, Z = 4.317, P < 0.001), Ktrans (AUC = 0.852, Z = 2.713, P = 0.007), and Ve (AUC = 0.808, Z = 3.175, P = 0.002) (Figure 4B, Table 5).
Bootstrapped samples were used to validate the combination of independent predictors. The ROC and the calibration curve indicated that the validation models not only had high accuracy in identifying TP53-mutant and TP53-wild early-stage EC (AUC, 0.815; 95% CI, 0.782 – 0.846, Figure 5A), and low-risk and risk early-stage EC (AUC, 0.922; 95% CI, 0.895 – 0.940, Figure 6A), but also highly had good consistency (Figure 5B, 6B). Also, DCA showed that the above combinations of independent predictors were both reliable clinical decision tools (Figure 5C, Figure 6C).
The parameter D of IVIM can reflect the diffusion movement of water molecules in the tissue, and usually, the more obvious the restriction of water molecule diffusion, the smaller the D value.13 In this study, the D value of the TP53-mutant group was significantly lower than that of the TP53-wild group, which was similar to the results of Wang
D* was a perfusion parameter of IVIM that is mainly correlated with the velocity of blood flow within the microcirculation.13 Previous publications have demonstrated that D* values with poor stability and repeatability could not effectively evaluate histopathological information of early-stage EC due to the influence of the scanning parameters, the ROI determination method, the signal-to-noise ratio (SNR), and other factors.17,18,19,20 In this study, there was no statistically significant difference in D* between the TP53-mutant and TP53-wild groups, and the low-risk and the non-low-risk groups, which was consistent with the above research, further proving that the D* value was unable to play a role in the assessment of TP53 status and risk stratification in early-stage EC.
As another perfusion parameter derived from IVIM, f was mainly related to the microvascular density of the tissue.13,27 A study by Zhang
Ktrans is the most significant perfusion-related parameter in DCE-MRI, mainly reflecting the transfer rate of the contrast agent from the vessel to the EES.30 Previous studies have shown that the more neovascularization in the tissue and the greater the permeability, the greater the Ktrans value.31 In terms of TP53 status assessment, the present study found a significantly higher Ktrans value in the TP53-mutant group compared with the TP53-wild group, which we suggest may be related to the ability of TP53 gene overexpression to promote angiogenesis.2,3 In terms of risk stratification assessment, several studies have shown that EC with aggressive characteristics, such as grade 3, advanced FIGO stage, and non-endometrioid sub-type, grows quickly without sufficient neoangiogenesis (i.e., blood support), resulting in tissue hypoxia. Hypoxia will lead to tissue necrosis and the formation of hypoperfused areas, thus eventually causing a decrease in overall tumor perfusion and a decrease in Ktrans values.12,17,32,33 In this work, the Ktrans value was significantly lower in the non-low-risk group than in the low-risk group, which was consistent with the above findings and further demonstrates that the Ktrans value can play a role in the risk stratification of early-stage EC.
Kep was designed to reflect the transfer rate of the contrast agent from the EES into vessels, so similar to Ktrans, its size was closely related to the number of new vessels and vascular permeability.29 In this study, since TP53 overexpression can promote angiogenesis2,3, the Kep value of the TP53-mutant group was significantly higher than those of the TP53-wild group, and the diagnostic efficacy was 0.773. However, the Kep value did not show significant value in the identification of different risk stratifications, which was not consistent with the study of Ye
Ve is a parameter in DCE-MRI that can reflect the volume of EES. In the present study, there was no significant difference in Ve between the TP53-mutant and TP53-wild groups, which may be related to the fact that TP53 overexpression promotes both cell proliferation and angiogenesis, resulting in difficulty in significant changes in EES.2,3 In terms of risk stratification assessment, Ve values in the non-low-risk group were significantly smaller than those in the low-risk group, which was similar to the results of previous studies17,34, and we speculated that the reason for this result may lie in the fact that the non-low-risk group had greater invasiveness and therefore greater cell density, tighter tissue structure, and smaller EEC compared with the low-risk group. However, some studies have also concluded that Ve was difficult to use in the evaluation of diseases such as EC and breast cancer.12,35 This may be related to the fact that Ve is less stable and susceptible to factors such as lesion edema and microcystic changes.36 In a follow-up study, we will expand the sample size and further explore the role of Ve in EC assessment to obtain more convincing results.
The diagnostic efficacy of the combination of independent predictors and each individual parameter was compared in this study, and the results showed that the diagnostic efficacy of the former was significantly higher than that of the latter, which may be because the combination of independent predictors concentrates the advantages of different parameters and therefore can reflect the lesion characteristics more comprehensively and accurately. Therefore, we suggest that the combined application of IVIM and DCE-MRI in clinical routine may provide a more reliable basis for the TP53 status and risk stratification prediction of early-stage EC when conditions permit.
In this study, TP53 mutation and risk stratification in early-stage EC were included in each other's regression analysis, and the results showed that neither was a predictor of the other. Although the small sample size may have affected the reliability of the above results to a certain extent, it indicates to some extent that the TP53 status in early-stage EC is not significantly correlated with risk stratification. In the future, as the sample size increases, we will conduct more in-depth studies on the relationship between the two, with a view to obtaining more accurate results.
This study has several limitations. First, our study was designed at a single institution with a relatively small number of patients, especially since some patients forgo immunohistochemical testing for financial reasons, which may have led to selection bias. Second, due to the small sample size, this study did not set up a separate validation set but used the bootstrap (1000 samples) method to validate the combination of independent predictors, which may have reduced the reliability of the experimental results. Third, areas of cystic degeneration, necrosis, apparent signs and hemorrhage artifacts, or vessels were avoided in the delineation of the ROI, which may influence the determination of some parameters. Finally, the machine used in this study was a 1.5 T MRI, and its imaging quality and parameter reliability may be inferior to those of a 3.0 T MRI.
Both DCE-MRI and IVIM facilitate the prediction of TP53 status and risk stratification in early-stage EC. Comparison with each single parameter, the combination of independent predictors provided better predictive power and may serve as a superior imaging marker.