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Expression of DNA-damage response and repair genes after exposure to DNA-damaging agents in isogenic head and neck cells with altered radiosensitivity

Vesna Todorovic
Vesna Todorovic
, 
Blaz Groselj
Blaz Groselj
, 
Maja Cemazar
Maja Cemazar
, 
Ajda Prevc
Ajda Prevc
, 
Martina Niksic Zakelj
Martina Niksic Zakelj
, 
Primoz Strojan
Primoz Strojan
 and 
Gregor Sersa
Gregor Sersa
   | Apr 07, 2022
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Article Category: Research Article
Published Online: Apr 07, 2022
Page range: 173 - 184
Received: Feb 25, 2022
Accepted: Mar 16, 2022
DOI: https://doi.org/10.2478/raon-2022-0014
© 2022 Vesna Todorovic, Blaz Groselj, Maja Cemazar, Ajda Prevc, Martina Niksic Zakelj, Primoz Strojan, Gregor Sersa, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Overall expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Symbols are mean gene expression for each of the 84 DNA-damage signaling genes included on the Human DNA Damage Signaling Pathway RT2 ProfilerTM PCR Array from 3 independent experiments. The lines indicate the median gene expression of all tested genes.* p < 0.05; *** p < 0.001; **** p < 0.0001
Overall expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Symbols are mean gene expression for each of the 84 DNA-damage signaling genes included on the Human DNA Damage Signaling Pathway RT2 ProfilerTM PCR Array from 3 independent experiments. The lines indicate the median gene expression of all tested genes.* p < 0.05; *** p < 0.001; **** p < 0.0001

Figure 2

2-D clustergram analysis of the expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Clusters observed at ¼ dendrogram branch height are shown as blocks of different color.
2-D clustergram analysis of the expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Clusters observed at ¼ dendrogram branch height are shown as blocks of different color.

Figure 3

Deregulated expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Only significantly deregulated genes are shown. Symbols are the mean and SEM from three independent experiments.* p < 0.05; ** p < 0.01; *** p < 0.001
Deregulated expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Only significantly deregulated genes are shown. Symbols are the mean and SEM from three independent experiments.* p < 0.05; ** p < 0.01; *** p < 0.001

Figure 4

Overall gene expression in (parental, HPV-negative) FaDu cells and (radioresistant) FaDu-RR cells in response to cisplatin (CDDP) and bleomycin (BLEO). Symbols are mean gene expression for each of the 84 DNA-damage signaling genes included on the Human DNA Damage Signaling Pathway RT2 ProfilerTM PCR Array from 3 independent experiments. The lines indicate the median gene expression of all tested genes.* p < 0.05; *** p < 0.001; **** p < 0.0001
Overall gene expression in (parental, HPV-negative) FaDu cells and (radioresistant) FaDu-RR cells in response to cisplatin (CDDP) and bleomycin (BLEO). Symbols are mean gene expression for each of the 84 DNA-damage signaling genes included on the Human DNA Damage Signaling Pathway RT2 ProfilerTM PCR Array from 3 independent experiments. The lines indicate the median gene expression of all tested genes.* p < 0.05; *** p < 0.001; **** p < 0.0001

Figure 5

2-D clustergram analysis of the expression of DNA-damage signaling genes in (parental) FaDu cells and (radioresistant) FaDu-RR cells after exposure to cisplatin (CDDP) or bleomycin (BLEO). Clusters observed at ¼ dendrogram branch height are shown as blocks of different color.
2-D clustergram analysis of the expression of DNA-damage signaling genes in (parental) FaDu cells and (radioresistant) FaDu-RR cells after exposure to cisplatin (CDDP) or bleomycin (BLEO). Clusters observed at ¼ dendrogram branch height are shown as blocks of different color.

Figure 6

Deregulated expression of DNA-damage signaling genes in (parental) FaDu cells and (radioresistant) FaDu-RR cells after exposure to cisplatin (CDDP) or bleomycin (BLEO). Only significantly deregulated genes are shown. Symbols are the mean and SEM from three independent experiments.* p < 0.05
Deregulated expression of DNA-damage signaling genes in (parental) FaDu cells and (radioresistant) FaDu-RR cells after exposure to cisplatin (CDDP) or bleomycin (BLEO). Only significantly deregulated genes are shown. Symbols are the mean and SEM from three independent experiments.* p < 0.05
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