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Expression of DNA-damage response and repair genes after exposure to DNA-damaging agents in isogenic head and neck cells with altered radiosensitivity

Vesna Todorovic

Vesna Todorovic

Department of Experimental Oncology, Institute of Oncology LjubljanaLjubljana, Slovenia
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Todorovic, Vesna
, 
Blaz Groselj

Blaz Groselj

  • Department of Radiation Oncology, Institute of Oncology LjubljanaLjubljana, Slovenia
  • Faculty of Medicine, University of LjubljanaLjubljana, Slovenia
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Groselj, Blaz
, 
Maja Cemazar

Maja Cemazar

  • Department of Experimental Oncology, Institute of Oncology LjubljanaLjubljana, Slovenia
  • Faculty of Health Sciences, University of PrimorskaIzola, Slovenia
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Cemazar, Maja
, 
Ajda Prevc

Ajda Prevc

Department of Experimental Oncology, Institute of Oncology LjubljanaLjubljana, Slovenia
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Prevc, Ajda
, 
Martina Niksic Zakelj

Martina Niksic Zakelj

Department of Experimental Oncology, Institute of Oncology LjubljanaLjubljana, Slovenia
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Zakelj, Martina Niksic
, 
Primoz Strojan

Primoz Strojan

  • Department of Radiation Oncology, Institute of Oncology LjubljanaLjubljana, Slovenia
  • Faculty of Medicine, University of LjubljanaLjubljana, Slovenia
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Strojan, Primoz
 and 
Gregor Sersa

Gregor Sersa

  • Department of Experimental Oncology, Institute of Oncology LjubljanaLjubljana, Slovenia
  • Faculty of Health Sciences, University of LjubljanaLjubljana, Slovenia
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Sersa, Gregor
  
Apr 07, 2022
Expression of DNA-damage response and repair genes after exposure to DNA-damaging agents in isogenic head and neck cells with altered radiosensitivity's Cover Image
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Article Category: Research Article
Published Online: Apr 07, 2022
Page range: 173 - 184
Received: Feb 25, 2022
Accepted: Mar 16, 2022
DOI: https://doi.org/10.2478/raon-2022-0014
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© 2022 Vesna Todorovic, Blaz Groselj, Maja Cemazar, Ajda Prevc, Martina Niksic Zakelj, Primoz Strojan, Gregor Sersa, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Overall expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Symbols are mean gene expression for each of the 84 DNA-damage signaling genes included on the Human DNA Damage Signaling Pathway RT2 ProfilerTM PCR Array from 3 independent experiments. The lines indicate the median gene expression of all tested genes.* p < 0.05; *** p < 0.001; **** p < 0.0001
Overall expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Symbols are mean gene expression for each of the 84 DNA-damage signaling genes included on the Human DNA Damage Signaling Pathway RT2 ProfilerTM PCR Array from 3 independent experiments. The lines indicate the median gene expression of all tested genes.* p < 0.05; *** p < 0.001; **** p < 0.0001

Figure 2

2-D clustergram analysis of the expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Clusters observed at ¼ dendrogram branch height are shown as blocks of different color.
2-D clustergram analysis of the expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Clusters observed at ¼ dendrogram branch height are shown as blocks of different color.

Figure 3

Deregulated expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Only significantly deregulated genes are shown. Symbols are the mean and SEM from three independent experiments.* p < 0.05; ** p < 0.01; *** p < 0.001
Deregulated expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Only significantly deregulated genes are shown. Symbols are the mean and SEM from three independent experiments.* p < 0.05; ** p < 0.01; *** p < 0.001

Figure 4

Overall gene expression in (parental, HPV-negative) FaDu cells and (radioresistant) FaDu-RR cells in response to cisplatin (CDDP) and bleomycin (BLEO). Symbols are mean gene expression for each of the 84 DNA-damage signaling genes included on the Human DNA Damage Signaling Pathway RT2 ProfilerTM PCR Array from 3 independent experiments. The lines indicate the median gene expression of all tested genes.* p < 0.05; *** p < 0.001; **** p < 0.0001
Overall gene expression in (parental, HPV-negative) FaDu cells and (radioresistant) FaDu-RR cells in response to cisplatin (CDDP) and bleomycin (BLEO). Symbols are mean gene expression for each of the 84 DNA-damage signaling genes included on the Human DNA Damage Signaling Pathway RT2 ProfilerTM PCR Array from 3 independent experiments. The lines indicate the median gene expression of all tested genes.* p < 0.05; *** p < 0.001; **** p < 0.0001

Figure 5

2-D clustergram analysis of the expression of DNA-damage signaling genes in (parental) FaDu cells and (radioresistant) FaDu-RR cells after exposure to cisplatin (CDDP) or bleomycin (BLEO). Clusters observed at ¼ dendrogram branch height are shown as blocks of different color.
2-D clustergram analysis of the expression of DNA-damage signaling genes in (parental) FaDu cells and (radioresistant) FaDu-RR cells after exposure to cisplatin (CDDP) or bleomycin (BLEO). Clusters observed at ¼ dendrogram branch height are shown as blocks of different color.

Figure 6

Deregulated expression of DNA-damage signaling genes in (parental) FaDu cells and (radioresistant) FaDu-RR cells after exposure to cisplatin (CDDP) or bleomycin (BLEO). Only significantly deregulated genes are shown. Symbols are the mean and SEM from three independent experiments.* p < 0.05
Deregulated expression of DNA-damage signaling genes in (parental) FaDu cells and (radioresistant) FaDu-RR cells after exposure to cisplatin (CDDP) or bleomycin (BLEO). Only significantly deregulated genes are shown. Symbols are the mean and SEM from three independent experiments.* p < 0.05
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