1. bookVolume 75 (2021): Issue 3 (June 2021)
Journal Details
License
Format
Journal
First Published
14 Sep 2008
Publication timeframe
6 times per year
Languages
English
access type Open Access

Influence of Macro- and Micro-Element Content on Mycelial Growth of Phoma Sp.1

Published Online: 22 Jul 2021
Page range: 206 - 210
Received: 08 Nov 2019
Accepted: 08 Dec 2020
Journal Details
License
Format
Journal
First Published
14 Sep 2008
Publication timeframe
6 times per year
Languages
English
Abstract

Phoma blight of conifer seedlings is poorly studied. The main causal agent in Belarus differs in DNA sequence from other described Phoma species, and has not been fully taxonomically described, and therefore has been temporarily designated Phoma sp. 1. The basic morphological and growth culture characteristics of Phoma sp. 1 have recently been published. This report provides additional data about the influence of macro- and micro-elements on the growth of Phoma sp. 1 in vitro to complement the description of growth culture and morphological features. It was found that addition of tricalcium phosphate and potassium chloride to the media at concentrations of 1 g/l and 2 g/l slows the mycelial growth by an average of 30%. The micro-elements copper sulphate (at 50 mg/l and 100 mg/l) and zinc sulphate (at 100 mg/l) decreased growth by 50%, 63%, and 32%, respectively. Urea completely inhibited Ph. sp.1 growth at 0.5 g/l. Summarising previously published information, Phoma sp. 1 is a mesophilic acidophile, capable of forming chlamydospores in culture. Pycnidia form deep in the media only after prolonged low-temperature storage. The optimal pH for growth is 4.3–4.9. Constant medium aeration favours the formation of typical mycelium, proportional growth and stable biomass accumulation.

Keywords

Aveskamp, M. (2014). Phylogeny and DNA-based identification in Phoma and related genera. PhD Thesis. Wageningen University, Wageningen, Netherlands. Search in Google Scholar

Baranov, O. Yu., Panteleev, S. V., Rubel, I. E., Yarmolovich, V. A., Seredich, M. O. (2015). Identification and annotation of repeating sequences in the genome of Phoma sp. 1. In: Collection of Scientific Papers “Problems of Forestry and Forest Management”, Vol. 75. Forest Institute of the National Academy of Sciences of Belarus, Gomel [Баранов, О. Ю., Пантелеев, С. В., Рубль, И. Е., Ярмолович, В. А., Середин, М. О. Идентификация и аннотация повторяющихся последовательностей в геноме Phoma sp. 1. В кн.: Сборник научных трудов “Проблемы лесоведения и лесоводства”, вып. 75. Институт леса Национальной академии наук Беларуси. Гомель], pp. 183–195 (in Russian). Search in Google Scholar

Baranov, O. Yu., Yarmolovich, V. A., Panteleev, S. V., Kupreenko, D. G. (2012). Molecular-genetic diagnostics of fungal diseases in forest nurseries. Lesnoe i Ohotnich’e Hozjajstvo [Баранов, О. Ю., Ярмолович, В. А., Пантелеев, С. В., Купреенко, Д. Г. Молекулярно-генетическая диагностика грибных болезней в лесных питомниках. Лесное и охотничье хозяйство], 6, 21–29 (in Russian). Search in Google Scholar

Boerema, G. H., de Gruyter, J., Noordeloos, M., Hamers, M. (eds.) (2004). Phoma Identification Manual. CABI Publishing, Cambridge. 470 pp. Search in Google Scholar

Fitt, B. D. L., Brun, H., Barbetti, M. J., Rimmer, S. R. (2006). World-wide importance of phoma stem canker (Leptosphaeria maculans and L. biglo-bosa) on oilseed rape (Brassica napus). Eur. J. Plant Pathol., 114 (1), 3–15. Search in Google Scholar

Gardes, M., Bruns, T. D. (1993). ITS primers with enhanced specificity for basidiomycetes — application to the identification of mycorrhizae and rusts. Mol. Ecol., 2 (2), 113–118. Search in Google Scholar

Hansen, E. M., Hamm, P. B. (1988). Canker diseases of Douglas-fir seedlings in Oregon and Washington bareroot nurseries. Can. J. Forest Res., 18 (8), 1053–1058. Search in Google Scholar

Jakimov, N. I., Gvozdev, V. K., Prahodskij, A. N. (2007). Forest Culture and Protective Forestry [Якимов, H. И., Гвоздев, В. К., Праходский, А. Н. Лесные культуры и защитноелесоразведение]. Belarusian State Technological University, Minsk. 311 pp. (in Russian). Search in Google Scholar

James, R. L. (1986). Mortality of Containerized Western Larch Seedlings at the Champion Timberlands Nursery, Plains, Montana (Report 8616). United States Forest Service, Montana. Search in Google Scholar

James, R. L. (1998). Phoma branch dieback of 30 bare root douglas-fir seedlings USDA forest service nursery, Coeur D’alene, Idaho. Nursery disease notes (No. 136). USDA Forest Service. Search in Google Scholar

Kliejunas, J. T., Allison, J. R., McCain, A. H., Smith, R. S. (1985). Phoma blight of fir and Douglas-fir seedlings in a California Nursery. Plant Dis., 69, 773–775. Search in Google Scholar

National Center for Biotechnological Information, NCBI (Electronic resource). http://www.ncbi.nlm.nih.gov (accessed 14 July 2019). Search in Google Scholar

Panteleev, S. V., Baranov, O. Yu., Yarmolovich, V. A., Seredich, M. O., Rubel, I. E. (2015). The analysis of microsatellite loci of the Phoma genome on the basis of full-genomic sequencing. In: Collection of Scientific Papers “Problems of Forestry and Forest Management”, Vol. 75. Forest Institute of the National Academy of Sciences of Belarus, Gomel [Пантелеев, С. В., Баранов, О. Ю., Ярмолович, В. А, Середин, М. О., Рубль, И. Е. Анализ микросателлитных локусов генома Phoma на основании полногеномного секвенирования. В кн.: Сборник научных трудов “Проблемы лесоведения и лесоводства”, Вып. 75. Институт леса Национальной академии наук Беларуси. Гомель], pp. 259-263 (in Russian). Search in Google Scholar

Seifert, K. A., Rossman, A. Y. (2010). How to describe a new fungal species. IMA Fungus, 1 (2), 109–116. Search in Google Scholar

Seredich, M.* (2016). Cultural and morphological characteristics of fungus Phoma sp. 1 – Phoma blight causing agents in coniferous tree seedlings. Proceedings of Belarusian State Technological University, 1, 98–101. Search in Google Scholar

Siaredzich, M. * (2017). Justification of actions for protection of planting material of Scots pine and Norway spruce against Phoma blight in forest nurseries in Belarus [Середич, M. О. Обоснование мероприятий по защите посадочного материала сосны обыкновенной и ели европейской от фомоза в лесных питомниках Беларуси]. Doctoral dissertation, Belarusian State Technological University, Minsk, Belarus. (in Russian). Search in Google Scholar

Šķipars, V., Siaredzich, M., Belevich, V., Bruņeviča, N., Brūna, L., Ruņģis, D. E. (2018). Genetic differentiation of Phoma sp. isolates using retrotransposon-based iPBS assays. Environ. Exp. Biol., 16, 307–314. Search in Google Scholar

Smith, I. M., McNamara, D. G., Scott, P. R., Harris, K. M. (eds.) (1992). Quarantine Pests for Europe. Data Sheets on Quarantine Pests for the European Communities and for the European and Mediterranean Plant Protection Organization. CAB International, Wallingford. 1032 pp. Search in Google Scholar

Srago, M. D., James, R. L., Kliejunas, J. T. (1989). Phoma blight. In: Cordell, C. E., Anderson, R. L., Hoffard, W. H., Landis, T. D., Smith Jr., R. S., Toko, H. V. (eds.). Forest Nursery Pests. Agriculture Handbook No. 680. U.S. Department of Agriculture, Forest Service, pp. 54–55. Search in Google Scholar

Yarmolovich, V., Baranov, O., Dishuk, N., Romanenko, M. * (2013). Phoma blight of planting material in forest nurseries. Lesnoe i Ohotnich’e Hozjajstvo [Ярмолович, В., Баранов, О., Дишук, Н., Романенко, M. Фомоз посадочного материала в лесных питомниках. Лесное и охотничье хозяйство], 3, 18–24 (in Russian). Search in Google Scholar

Zavertkina, I. V. (2007). Biological features of the Siberian population of Phoma exiqua var. foveata and improvement of potato protection system from phoma blight [Заверткина, И. В. Биологические особенности сибирской популяции Phoma exiqua var. foveata и совершенствование системы защиты картофеля от фомоза]. Doctoral dissertation. Novosibirsk State Agricultural University, Kinel, Russian Federation (in Russian). Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo