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Eco-friendly high-rate formation of silver nanoparticles in agave inulin and its bactericidal effect against Escherichia coli

María Teresa Sánchez-Vieyra
María Teresa Sánchez-Vieyra
, 
Miguel Ojeda-Martínez
Miguel Ojeda-Martínez
, 
Eden Oceguera-Contreras
Eden Oceguera-Contreras
, 
Sergio Yair Rodríguez-Preciado
Sergio Yair Rodríguez-Preciado
, 
Mariana Díaz-Zaragoza
Mariana Díaz-Zaragoza
, 
Brenda Esmeralda Martínez-Zérega
Brenda Esmeralda Martínez-Zérega
, 
José Luis González-Solís
José Luis González-Solís
 and 
David Omar Oseguera-Galindo
David Omar Oseguera-Galindo
   | Jan 09, 2024
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Published Online: Jan 09, 2024
Page range: 62 - 73
Received: Jul 03, 2023
Accepted: Nov 25, 2023
DOI: https://doi.org/10.2478/msp-2023-0034
Keywords
© 2023 María Teresa Sánchez-Vieyra et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Experimental setup for the synthesis of AgNPs. Variables were (1) agave inulin; (2) AgNO3; (3) temperature; (4) pH
Experimental setup for the synthesis of AgNPs. Variables were (1) agave inulin; (2) AgNO3; (3) temperature; (4) pH

Fig. 2.

UV-Vis absorbance of nanoparticles obtained using different agave inulin concentrations
UV-Vis absorbance of nanoparticles obtained using different agave inulin concentrations

Fig. 3.

UV-Vis absorbance of nanoparticles obtained using different AgNO3 concentration
UV-Vis absorbance of nanoparticles obtained using different AgNO3 concentration

Fig. 4.

UV-Vis absorbance of nanoparticles obtained using different temperatures
UV-Vis absorbance of nanoparticles obtained using different temperatures

Fig. 5.

(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using a temperature of 75°C
(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using a temperature of 75°C

Fig. 6.

UV-Vis absorbance of nanoparticles obtained using different pH values
UV-Vis absorbance of nanoparticles obtained using different pH values

Fig. 7.

(a) UV-Vis spectra of nanoparticles obtained with a pH of 12 using different solutions in water. (b) Line calibration of the maximum absorbance versus the agave inulin concentration (mg/mL)
(a) UV-Vis spectra of nanoparticles obtained with a pH of 12 using different solutions in water. (b) Line calibration of the maximum absorbance versus the agave inulin concentration (mg/mL)

Fig. 8.

(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using pH 11
(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using pH 11

Fig. 9.

(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using pH 10
(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using pH 10

Fig. 10.

(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using a pH of 12
(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using a pH of 12

Fig. 11.

Bactericidal effect. LB medium was used to determine the bactericidal effect. (1) Positive control: LB medium; (2) Negative control: LB medium and 100 μL E. coli strain; (3) LB medium, 100 μL E. coli strain, and 100 μL agave inulin; (4) LB medium, 100 μL of E. coli strain, and 100 μL of AgNPs (S1bac); (5) LB medium, 100 μL of E. coli strain, and 100 μL of AgNPs (S2bac); (6) LB medium, 100 μL E. Coli strain, and 100 μL agave inulin
Bactericidal effect. LB medium was used to determine the bactericidal effect. (1) Positive control: LB medium; (2) Negative control: LB medium and 100 μL E. coli strain; (3) LB medium, 100 μL E. coli strain, and 100 μL agave inulin; (4) LB medium, 100 μL of E. coli strain, and 100 μL of AgNPs (S1bac); (5) LB medium, 100 μL of E. coli strain, and 100 μL of AgNPs (S2bac); (6) LB medium, 100 μL E. Coli strain, and 100 μL agave inulin

Parameters and values used in the preparation of samples to study agave inulin’s effect on the synthesis of AgNPs

Sample A B C D
S1A 6 1 23 6.5
S2A 12 1 23 6.5
S3A 18 1 23 6.5
S4A 24 1 23 6.5

Parameters and values used in the preparation of samples to study pH effect on the synthesis of AgNPs

Sample A B C D
S1D 20 1 23 12
S2D 20 1 23 11
S3D 20 1 23 10
S4D 20 1 23 9.3
S5D 20 1 23 8.3

Contents of the test tubes to study antibacterial activity

Tube LB (mL) E. coli (μL) Inulin (μL) Sample (μL)
1 5 – – –
2 5 100 – –
3 5 100 100 –
4 5 100 – S1bac (100)
5 5 100 – S2bac (100)
6 5 100 100 –

Parameters and values used in the preparation of samples to study the AgNO3 effect on the synthesis of AgNPs

Sample A B C D
S1B 20 1 23 6.5
S2B 20 3 23 6.5
S3B 20 5 23 6.5

Parameters and values used in the preparation of samples to study temperature effect on the synthesis of AgNPs

Sample A B C D
S1C 20 1 23 6.5
S2C 20 1 40 6.5
S3C 20 1 60 6.5
S4C 20 1 75 6.5
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