Eco-friendly high-rate formation of silver nanoparticles in agave inulin and its bactericidal effect against Escherichia coli

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Parameters and values used in the preparation of samples to study agave inulin’s effect on the synthesis of AgNPs

Sample	A	B	C	D
S1A	6	1	23	6.5
S2A	12	1	23	6.5
S3A	18	1	23	6.5
S4A	24	1	23	6.5

Parameters and values used in the preparation of samples to study pH effect on the synthesis of AgNPs

Sample	A	B	C	D
S1D	20	1	23	12
S2D	20	1	23	11
S3D	20	1	23	10
S4D	20	1	23	9.3
S5D	20	1	23	8.3

Contents of the test tubes to study antibacterial activity

Tube	LB (mL)	E. coli (μL)	Inulin (μL)	Sample (μL)
1	5	–	–	–
2	5	100	–	–
3	5	100	100	–
4	5	100	–	S1bac (100)
5	5	100	–	S2bac (100)
6	5	100	100	–

Parameters and values used in the preparation of samples to study the AgNO3 effect on the synthesis of AgNPs

Sample	A	B	C	D
S1B	20	1	23	6.5
S2B	20	3	23	6.5
S3B	20	5	23	6.5

Parameters and values used in the preparation of samples to study temperature effect on the synthesis of AgNPs

Sample	A	B	C	D
S1C	20	1	23	6.5
S2C	20	1	40	6.5
S3C	20	1	60	6.5
S4C	20	1	75	6.5