<abstract xmlns="http://www.w3.org/1999/xhtml">

Stress can be a reason for some physiological and biological disorders in the body. The antioxidative defense system is necessary for the maintenance of redox homeostasis in the organisms. Alkaline water (AW) is in the focus of the scientific interest due to its antioxidative effect. The treatment with AW and sodium ascorbate (SA) is expected to have potential preventive effect on the organism to hyperthermic stress. The aim of this study was to investigate the effect of AW and SA on glucose and cortisol levels during acute hyperthermic stress, in white female Wistar laboratory rats. The rats were divided into three groups, each having 10 subjects. They were exposed in hyperthermic conditions (41˚C) for 80 min, in 21 consecutive days in order to induce oxidative stress. The first group received drinkable water (control group), the second AW, and the third, AW and SA. Plasma glucose levels were determined by colorimetric method. Cortisol level was measured by the enzyme-linked immunosorbent assay method (ELISA). The means were compared using the Tukey test. Differences were considered significant at a level of p&lt;0.05. Our results showed that levels of glucose and cortisol were significantly higher in the group treated with AW on the 21st day after treatment (p&lt;0.0001), but not on the 7th and 14th day as compared to the control group. Also, co-treatment of animals with AW and SA had significantly increased the levels of glucose and cortisol on the 21st day after treatment, indicating a synergistic effect. In conclusion, the individual action of AW or in synergism with SA caused a high protective effect on oxidative damage in white Wistar laboratory rats.
</abstract>

Stress can be a reason for some physiological and biological disorders in the body. The antioxidative defense system is necessary for the maintenance of redox homeostasis in the organisms. Alkaline water (AW) is in the focus of the scientific interest due to its antioxidative effect. The treatment with AW and sodium ascorbate (SA) is expected to have potential preventive effect on the organism to hyperthermic stress. The aim of this study was to investigate the effect of AW and SA on glucose and cortisol levels during acute hyperthermic stress, in white female Wistar laboratory rats. The rats were divided into three groups, each having 10 subjects. They were exposed in hyperthermic conditions (41˚C) for 80 min, in 21 consecutive days in order to induce oxidative stress. The first group received drinkable water (control group), the second AW, and the third, AW and SA. Plasma glucose levels were determined by colorimetric method. Cortisol level was measured by the enzyme-linked immunosorbent assay method (ELISA). The means were compared using the Tukey test. Differences were considered significant at a level of p<0.05. Our results showed that levels of glucose and cortisol were significantly higher in the group treated with AW on the 21st day after treatment (p<0.0001), but not on the 7th and 14th day as compared to the control group. Also, co-treatment of animals with AW and SA had significantly increased the levels of glucose and cortisol on the 21st day after treatment, indicating a synergistic effect. In conclusion, the individual action of AW or in synergism with SA caused a high protective effect on oxidative damage in white Wistar laboratory rats.

The Effect of Alkaline Water and Sodium Ascorbate on Glucose and Cortisol Levels During Acute Hyperthermic Stress in White Laboratory Rats

Department of Biochemistry and Physiology, Faculty of Natural Sciences and Mathematics

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Stress can be a reason for some physiological and biological disorders in the body. The antioxidative defense system is necessary for the maintenance of redox...

The Effect of Alkaline Water and Sodium Ascorbate on Glucose and Cortisol Levels During Acute Hyperthermic Stress in White Laboratory Rats

Published Online: Oct 26, 2021

Page range: 179 - 185

Received: May 12, 2021

Accepted: Jul 14, 2021

DOI: https://doi.org/10.2478/macvetrev-2021-0023

Keywords
alkaline water, sodium ascorbate, hyperthermic stress, glucose, cortisol

© 2021 Valdrina Ajeti et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The Effect of Alkaline Water and Sodium Ascorbate on Glucose and Cortisol Levels During Acute Hyperthermic Stress in White Laboratory Rats

Published Online: Oct 26, 2021

Page range: 179 - 185

Received: May 12, 2021

Accepted: Jul 14, 2021

DOI: https://doi.org/10.2478/macvetrev-2021-0023

Keywordsalkaline water, sodium ascorbate, hyperthermic stress, glucose, cortisol

© 2021 Valdrina Ajeti et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
alkaline water, sodium ascorbate, hyperthermic stress, glucose, cortisol