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Chemical coding of piglets small intestine neurons after prenatal exposure to β-hydroxy-β-methylbutyrate

Aleksandra Dajnowska

Aleksandra Dajnowska

Department of Animal Anatomy and Histology, Department of Animal Physiology, Institute of Animal Nutrition and Bromatology, Faculty of Veterinary Medicine, Department of Biophysics, Faculty of Environmental Biology, University of Life Sciences in LublinLublin, Poland
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Dajnowska, Aleksandra
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Katarzyna Kras

Katarzyna Kras

Department of Animal Anatomy and Histology, Department of Animal Physiology, Institute of Animal Nutrition and Bromatology, Faculty of Veterinary Medicine, Department of Biophysics, Faculty of Environmental Biology, University of Life Sciences in LublinLublin, Poland
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Kras, Katarzyna
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Ewa Tomaszewska

Ewa Tomaszewska

Department of Animal Physiology, Institute of Animal Nutrition and Bromatology, Faculty of Veterinary Medicine, Department of Biophysics, Faculty of Environmental Biology, University of Life Sciences in LublinLublin, Poland
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Tomaszewska, Ewa
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Piotr Dobrowolski

Piotr Dobrowolski

Department of Functional Anatomy and Cytobiology, Maria Curie-Skłodowska UniversityLublin, Poland
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Dobrowolski, Piotr
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Renata Klebaniuk

Renata Klebaniuk

Institute of Animal Nutrition and Bromatology, Faculty of Veterinary Medicine, Department of Biophysics, Faculty of Environmental Biology, University of Life Sciences in LublinLublin, Poland
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Klebaniuk, Renata
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Siemowit Muszyński

Siemowit Muszyński

Department of Biophysics, Faculty of Environmental Biology, University of Life Sciences in LublinLublin, Poland
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Muszyński, Siemowit
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Marcin Bartłomiej Arciszewski

Marcin Bartłomiej Arciszewski

Department of Animal Anatomy and Histology, Department of Animal Physiology, Institute of Animal Nutrition and Bromatology, Faculty of Veterinary Medicine, Department of Biophysics, Faculty of Environmental Biology, University of Life Sciences in LublinLublin, Poland
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Arciszewski, Marcin Bartłomiej
  
Apr 19, 2025
Chemical coding of piglets small intestine neurons after prenatal exposure to β-hydroxy-β-methylbutyrate's Cover Image
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Published Online: Apr 19, 2025
Page range: 249 - 255
Received: Nov 11, 2024
Accepted: Apr 01, 2025
DOI: https://doi.org/10.2478/jvetres-2025-0024
Keywords
© 2025 Aleksandra Dajnowska et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1.

Prenatal effect of β-hydroxy-β-methylbutyrate (HMB) supplementation on the area occupied by cocaine-and-amphetamine-regulated transcript (CART)-immunoreactive (IR), substance P (SP)-IR, vasoactive intestinal peptide (VIP)-IR, and neuronal nitric oxide synthase (nNOS)-IR nerve fibres in the muscular layer of the duodenum, jejunum and ileum (a, b and c), the submucosa of the duodenum, jejunum and ileum (d, e and f), and the mucosa of the duodenum, jejunum and ileum (g, h and i) in newborn piglets prenatally supplemented with HMB and control piglets. * – significant difference between the control and HMB groups at P-value < 0.05; ** – at P-value < 0.01; *** – at P-value < 0.001
Prenatal effect of β-hydroxy-β-methylbutyrate (HMB) supplementation on the area occupied by cocaine-and-amphetamine-regulated transcript (CART)-immunoreactive (IR), substance P (SP)-IR, vasoactive intestinal peptide (VIP)-IR, and neuronal nitric oxide synthase (nNOS)-IR nerve fibres in the muscular layer of the duodenum, jejunum and ileum (a, b and c), the submucosa of the duodenum, jejunum and ileum (d, e and f), and the mucosa of the duodenum, jejunum and ileum (g, h and i) in newborn piglets prenatally supplemented with HMB and control piglets. * – significant difference between the control and HMB groups at P-value < 0.05; ** – at P-value < 0.01; *** – at P-value < 0.001

Fig. 2.

Representative photomicrographs of the immunohistochemical reactions for cocaine-and-amphetamine-regulated transcript (CART) (A and B), substance P (SP) (C and D), vasoactive intestinal peptide (VIP) (E and F) and neuronal nitric oxide synthase (nNOS) (G and H) in the duodenum (A, B, E and F) and jejunum (C, D, G and H) of control (A, C, E and G) and prenatally HMB-exposed (B, D, F and H) piglets. Arrow – positive reaction. Scale bar = 200 μm
Representative photomicrographs of the immunohistochemical reactions for cocaine-and-amphetamine-regulated transcript (CART) (A and B), substance P (SP) (C and D), vasoactive intestinal peptide (VIP) (E and F) and neuronal nitric oxide synthase (nNOS) (G and H) in the duodenum (A, B, E and F) and jejunum (C, D, G and H) of control (A, C, E and G) and prenatally HMB-exposed (B, D, F and H) piglets. Arrow – positive reaction. Scale bar = 200 μm

Primary and secondary antibodies used in the study

Antibody Host Catalogue number Dilution Manufacturer
Primary antibody
anti-VIP rabbit ab22736 1:400 Abcam Limited, Cambridge, UK
anti-CART rabbit H-003-62 1:2,000 Phoenix Pharmaceuticals, Burlingame, CA, USA
anti-nNOS rabbit 160870 1:200 Cayman Chemical, Ann Arbor, MI, USA
anti-SP mouse ab14184 1:1,000 Abcam Limited, Cambridge, UK
Secondary antibody
anti-mouse/anti-rabbit goat DPVB-HRP RTU ImmunoLogic, Duiven, the Netherlands
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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