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The influence of probiotic administration on the phagocytic and oxidative burst activity of neutrophils and monocytes in the peripheral blood of dairy cows during different lactation periods

Piotr Brodzki

Piotr Brodzki

Department and Clinic of Animal Reproduction, University of Life Sciences in LublinLublin, Poland
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Brodzki, Piotr
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Hubert Gorzkoś

Hubert Gorzkoś

Private Veterinary Practice, GorvetSztutowo, Poland
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Gorzkoś, Hubert
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Jan Marczuk

Jan Marczuk

Department and Clinic of Animal Internal Medicine, University of Life Sciences in LublinLublin, Poland
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Marczuk, Jan
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Urszula Lisiecka

Urszula Lisiecka

Department of Epizootiology and Clinic of Infectious Diseases, University of Life Sciences in LublinLublin, Poland
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Lisiecka, Urszula
, 
Andrzej Junkuszew

Andrzej Junkuszew

Department of Breeding of Small Ruminants, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in LublinLublin, Poland
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Junkuszew, Andrzej
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Leszek Krakowski

Leszek Krakowski

Department and Clinic of Animal Reproduction, University of Life Sciences in LublinLublin, Poland
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Krakowski, Leszek
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Marek Szczubiał

Marek Szczubiał

Department and Clinic of Animal Reproduction, University of Life Sciences in LublinLublin, Poland
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Szczubiał, Marek
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Nikodem Brodzki

Nikodem Brodzki

Faculty of Veterinary Medicine, University of Life Sciences in LublinLublin, Poland
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Brodzki, Nikodem
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Katarzyna Głodkowska

Katarzyna Głodkowska

Department and Clinic of Animal Reproduction, University of Life Sciences in LublinLublin, Poland
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Głodkowska, Katarzyna
  
Aug 08, 2024
The influence of probiotic administration on the phagocytic and oxidative burst activity of neutrophils and monocytes in the peripheral blood of dairy cows during different lactation periods's Cover Image
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Published Online: Aug 08, 2024
Page range: 401 - 408
Received: Jan 18, 2024
Accepted: Jul 31, 2024
DOI: https://doi.org/10.2478/jvetres-2024-0043
Keywords
© 2024 P. Brodzki et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Cytometric analysis of phagocytosis activity of neutrophils (A and B) and monocytes (C and D) in the peripheral blood of cows at various periods of lactation (mean ± standard error of the mean). DBD – day before drying; DBP – day before parturition; DPP – day postpartum; Experimental – cows provided with probiotic (n = 10); Control – cows without probiotic (n = 10); Phagocytic cells (%) – percentage of neutrophils and monocytes that had engulfed bacteria; MFI – mean fluorescence intensities expressing individual cellular phagocytic activity (number of bacteria per cell). Statistical significance – * p ≤ 0.05; ** p ≤ 0.01 with respect to the control; a–c – significant differences between the results for the material collected at different times in the group (P-value < 0.01)
Cytometric analysis of phagocytosis activity of neutrophils (A and B) and monocytes (C and D) in the peripheral blood of cows at various periods of lactation (mean ± standard error of the mean). DBD – day before drying; DBP – day before parturition; DPP – day postpartum; Experimental – cows provided with probiotic (n = 10); Control – cows without probiotic (n = 10); Phagocytic cells (%) – percentage of neutrophils and monocytes that had engulfed bacteria; MFI – mean fluorescence intensities expressing individual cellular phagocytic activity (number of bacteria per cell). Statistical significance – * p ≤ 0.05; ** p ≤ 0.01 with respect to the control; a–c – significant differences between the results for the material collected at different times in the group (P-value < 0.01)

Fig. 2.

Cytometric analysis of oxidative burst activity of neutrophils (A and B) in the peripheral blood of cows at various periods of lactation (mean ± standard error of the mean). DBD – day before drying; DBP – day before parturition; DPP – day postpartum; Experimental – cows provided with probiotic (n = 10); Control – cows without probiotic (n = 10); Phagocytic cells (%) – the percentage of phagocytic cells that produced reactive oxygen substrates (measured by the conversion of DHR123 to R123); MFI – mean fluorescence intensities expressing enzymatic activity of phagocytic cells (measured by the amount of R123 per cell). Statistical significance – * p ≤ 0.05; ** p ≤ 0.01 with respect to the control; a–c – differences between the results for the material collected at different times in the group (P-value < 0.01)
Cytometric analysis of oxidative burst activity of neutrophils (A and B) in the peripheral blood of cows at various periods of lactation (mean ± standard error of the mean). DBD – day before drying; DBP – day before parturition; DPP – day postpartum; Experimental – cows provided with probiotic (n = 10); Control – cows without probiotic (n = 10); Phagocytic cells (%) – the percentage of phagocytic cells that produced reactive oxygen substrates (measured by the conversion of DHR123 to R123); MFI – mean fluorescence intensities expressing enzymatic activity of phagocytic cells (measured by the amount of R123 per cell). Statistical significance – * p ≤ 0.05; ** p ≤ 0.01 with respect to the control; a–c – differences between the results for the material collected at different times in the group (P-value < 0.01)

Composition of the total mixed ration (TMR) and dry mass (DM) daily feed ration for lactating cows

Dose component Amount per cow, daily
kg TMR kg DM
Maize silage 25.0 8.8
Haylage 8.0 3.2
Ensiled brewery spent grain 8.0 2.7
Wheat straw 0.8 0.7
Ensiled maize grain 2.5 1.7
Ground barley grain 1.5 1.3
Ground triticale grain 1.5 1.3
Ground rapeseed 2.7 2.2
Extracted soyabean meal 2.0 1.7
Glycerine 0.3 0.24
Vitamin and mineral mixture 0.2 0.18
Sodium bicarbonate 0.2 0.2
Calcium carbonate 0.05 0.05
Total 52.75 24.27
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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