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Analysis of the role of Dirofilaria repens macrophage migration inhibitory factors in host–parasite interactions

Justyna Karabowicz

Justyna Karabowicz

Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life SciencesWarszawa, Poland
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Karabowicz, Justyna
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Ewa Długosz

Ewa Długosz

Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life SciencesWarszawa, Poland
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Długosz, Ewa
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Piotr Bąska

Piotr Bąska

Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life SciencesWarszawa, Poland
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Bąska, Piotr
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Mateusz Pękacz

Mateusz Pękacz

Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life SciencesWarszawa, Poland
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Pękacz, Mateusz
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Magdalena Elżbieta Wysmołek

Magdalena Elżbieta Wysmołek

Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life SciencesWarszawa, Poland
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Wysmołek, Magdalena Elżbieta
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Maciej Klockiewicz

Maciej Klockiewicz

Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life SciencesWarszawa, Poland
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Klockiewicz, Maciej
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Marcin Wiśniewski

Marcin Wiśniewski

Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life SciencesWarszawa, Poland
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Wiśniewski, Marcin
  
Sep 23, 2024
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Published Online: Sep 23, 2024
Page range: 381 - 388
Received: Apr 02, 2024
Accepted: Jul 11, 2024
DOI: https://doi.org/10.2478/jvetres-2024-0038
Keywords
© 2024 Justyna Karabowicz et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Dirofilaria repens is a zoonotic parasitic filarial nematode that infects carnivores and occasionally humans. Knowledge of the host–parasite molecular interactions enabling the parasite’s avoidance of the host immune response in subcutaneous dirofilariasis remains limited. Parasitic orthologues of host macrophage migration inhibitory factor (MIF) are molecules potentially involved in this process.

Material and Methods

Complementary DNA encoding two D. repens MIF orthologues (rDre-MIF-1 and rDre-MIF-2) was cloned into a pET-28a expression vector. The recombinant proteins were produced in Escherichia coli and purified using affinity nickel chromatography. The reactivity of both recombinant proteins was analysed with infected dog and immunised mouse sera.

Results

Stronger antibody production was induced by rDre-MIF-1 in mice, as evidenced by significantly higher levels of anti-rDre-MIF-1 total IgG, IgG2 and IgE antibodies than of anti-rDre-MIF-2 immunoglobulins. Additionally, a significantly different level of antibodies specific to both proteins was noted between the sera of infected dogs and those of uninfected dogs.

Conclusion

This study is the first attempt to characterise MIF orthologues from the filarial parasite D. repens, which may affect the immune response during infection.
Language:
English
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4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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