Origin of exosomes | BLV infection | BLV markers | Cellular markers | ||||
---|---|---|---|---|---|---|---|
gp51 | p24 | CD63 | CD9 | flotillin-1 | |||
Positive control – supernatant of FLK-BLV culture | + | + | + | + | + | + | |
Supernatant of cultured |
1 | + | + | + | + | + | + |
2 | + | + | + | + | + | + | |
3 | + | + | + | + | + | + | |
4 | + | + | + | + | + | + | |
5 | + | + | + | + | + | + | |
Negative control – plasma BLV− | − | − | − | + | + | + | |
Positive control – lysate of FLK–BLV cells | + | + | + | + | + | + | |
Bovine BLV+ sera (1–7) and BLV− sera (8–11) | 1 | + | + | + | + | + | + |
2 | + | + | + | + | + | + | |
3 | + | + | + | + | + | + | |
4 | + | + | + | + | + | + | |
5 | + | + | + | + | + | + | |
6 | + | + | + | + | + | + | |
7 | + | + | + | + | + | + | |
8 | − | − | − | + | + | + | |
9 | − | − | − | + | + | + | |
10 | − | − | − | + | + | + | |
11 | − | − | − | + | + | + |