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Cytokine secretion in stem cells of cattle infected with bovine leukaemia virus

Maria Szczotka

Maria Szczotka

Department of Biochemistry, National Veterinary Research InstitutePuławy, Poland
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Szczotka, Maria
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Jacek Kuźmak

Jacek Kuźmak

Department of Biochemistry, National Veterinary Research InstitutePuławy, Poland
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Kuźmak, Jacek
  
Mar 23, 2024
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Published Online: Mar 23, 2024
Page range: 19 - 33
Received: Aug 29, 2023
Accepted: Feb 28, 2024
DOI: https://doi.org/10.2478/jvetres-2024-0012
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© 2024 Maria Szczotka et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Cytokine expression of CD34+ stem cells isolated from the blood of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma
Cytokine expression of CD34+ stem cells isolated from the blood of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Fig. 2.

Cytokine expression in CD34+ stem cells isolated from the bone marrow of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma
Cytokine expression in CD34+ stem cells isolated from the bone marrow of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Fig. 3.

Cytokine expression in CD34+ stem cells isolated from the spleen of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma
Cytokine expression in CD34+ stem cells isolated from the spleen of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Fig. 4.

Cytokine expression in CD34+ stem cells isolated from the lymph nodes of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma
Cytokine expression in CD34+ stem cells isolated from the lymph nodes of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Fig. 5.

The cytokine levels in culture of CD34+ stem cells isolated from the blood of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma
The cytokine levels in culture of CD34+ stem cells isolated from the blood of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Fig. 6.

Results of two-way analysis of variance of the cytokine levels in culture of CD34+ stem cells isolated from the blood of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma
Results of two-way analysis of variance of the cytokine levels in culture of CD34+ stem cells isolated from the blood of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Fig. 7.

Results of two-way analysis of variance of the cytokine levels in culture of CD34+ stem cells isolated from the bone marrow of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma
Results of two-way analysis of variance of the cytokine levels in culture of CD34+ stem cells isolated from the bone marrow of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Fig. 8.

The cytokine levels in culture of CD34+ stem cells isolated from the spleens of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma
The cytokine levels in culture of CD34+ stem cells isolated from the spleens of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Fig. 9.

Results of two-way analysis of variance of the cytokine levels in cultures of CD34+ stem cells isolated from the spleens of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma
Results of two-way analysis of variance of the cytokine levels in cultures of CD34+ stem cells isolated from the spleens of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Fig. 10.

The cytokine levels in cultures of CD34+ stem cells isolated from the lymph nodes of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma
The cytokine levels in cultures of CD34+ stem cells isolated from the lymph nodes of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Fig. 11.

Results of two-way analysis of variance of the cytokine levels in cultures of CD34+ stem cells isolated from the lymph nodes of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma
Results of two-way analysis of variance of the cytokine levels in cultures of CD34+ stem cells isolated from the lymph nodes of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Fig. 12.

The expression of bovine leukaemia virus glycoprotein 51 in foetal lamb kidney cells permanently infected with BLV
The expression of bovine leukaemia virus glycoprotein 51 in foetal lamb kidney cells permanently infected with BLV

Fig. 13.

The expression of bovine leukaemia virus glycoprotein 51 in CD34+ blood stem cells
The expression of bovine leukaemia virus glycoprotein 51 in CD34+ blood stem cells

Fig. 14.

The expression of bovine leukaemia virus glycoprotein 51 in CD34+ bone marrow stem cells
The expression of bovine leukaemia virus glycoprotein 51 in CD34+ bone marrow stem cells

Fig. 15.

The expression of bovine leukaemia virus glycoprotein 51 in CD34+ spleen stem cells
The expression of bovine leukaemia virus glycoprotein 51 in CD34+ spleen stem cells

Fig. 16.

The expression of bovine leukaemia virus glycoprotein 51 in CD34+ lymph node stem cells
The expression of bovine leukaemia virus glycoprotein 51 in CD34+ lymph node stem cells

Fig. 17.

Lack of fluorescence in the control cells
Lack of fluorescence in the control cells

Mean values of cytokine secretion by bovine leukaemia virus–infected and uninfected CD34+ stem cells cultured in vitro

Sample type and presence of provirus (+/-) Mean secretion (pg/mL)
IL-6 IL-10 IL-12p40 IL-12p70 TNF-α IFN-γ
Blood BLV+ 1.90 3.42 27.01 5.30 3.36 2.22
BLV- 1.45 5.44 29.13 2.44 2.33 2.44
Bone marrow BLV+ 1.63 2.72 18.84 2.13 2.94 2.74
BLV- 1.89 2.70 17.12 1.13 2.47 2.26
Spleen BLV+ 0.33 1.87 53.03 1.46 3.82 1.67
BLV- 1.68 0.86 103.20 3.53 4.40 3.67
Lymph node BLV+ 1.21 1.36 49.81 2.19 3.72 1.93
BLV- 0.78 0.71 40.52 2.09 3.89 1.90
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