Serological characterisation of Lagovirus virus-like particles originating from native and mutated VP60 of rabbit haemorrhagic disease virus 2 and European brown hare syndrome virus
, , and | Mar 23, 2024
Published Online: Mar 23, 2024
Page range: 9 - 17
Received: Oct 19, 2023
Accepted: Mar 14, 2024
DOI: https://doi.org/10.2478/jvetres-2024-0019
Keywords
© 2024 Martyna Krejmer-Rąbalska et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Since lagoviruses cannot be cultivated
Material and Methods
Virus-like particles (VLPs) were produced in a baculovirus expression system in
Results
Protein production was confirmed by Western blot analysis using rabbit or hare sera and ELISA tests with different types of monoclonal antibody. The haemagglutination properties of some VLPs were also evaluated. Electron microscopy of wild-type EBHSV, wild-type RHDV2 and the four VP60 variants produced in this experiment revealed the formation of characteristic VLP structures.
Conclusion
For the first time, mutated VLPs of RHDV2 with an RGD motif in the VP60 sequence were obtained, which could potentially be used to deliver cargo to eukaryotic cells. Virus-like particles based on the VP60 proteins of EBHSV and RHDV with a three-lysine substitution in the S or P2 domains were also obtained. Potential exists for VLPs of EBHSV and RHDV2 as vaccine candidates.