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Molecular identification of blaTEM and blaCTX-M genes in multidrug-resistant Escherichia coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia

Agus Widodo

Agus Widodo

  • Faculty of Veterinary Medicine, Universitas AirlanggaSurabaya, Indonesia
  • Department of Health, Faculty of Vocational Studies, Universitas AirlanggaSurabaya, Indonesia
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Widodo, Agus
, 
Mirni Lamid

Mirni Lamid

Department of Animal Husbandry, Faculty of Veterinary Medicine, Universitas AirlanggaSurabaya, Indonesia
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Lamid, Mirni
, 
Mustofa Helmi Effendi

Mustofa Helmi Effendi

Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas AirlanggaSurabaya, Indonesia
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Effendi, Mustofa Helmi
, 
Wiwiek Tyasningsih

Wiwiek Tyasningsih

Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas AirlanggaSurabaya, Indonesia
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Tyasningsih, Wiwiek
, 
Dadik Raharjo

Dadik Raharjo

Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas AirlanggaSurabaya, Indonesia
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Raharjo, Dadik
, 
Aswin Rafif Khairullah

Aswin Rafif Khairullah

Department of Animal Husbandry, Faculty of Veterinary Medicine, Universitas AirlanggaSurabaya, Indonesia
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Khairullah, Aswin Rafif
, 
Shendy Canadya Kurniawan

Shendy Canadya Kurniawan

Department of Animal Sciences, Wageningen University and ResearchWageningen, Netherlands
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Kurniawan, Shendy Canadya
, 
Lita Rakhma Yustinasari

Lita Rakhma Yustinasari

Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas AirlanggaSurabaya, Indonesia
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Yustinasari, Lita Rakhma
, 
Katty Hendriana Priscilia Riwu

Katty Hendriana Priscilia Riwu

Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas MandalikaMataram, Indonesia
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Riwu, Katty Hendriana Priscilia
 and 
Otto Sahat Martua Silaen

Otto Sahat Martua Silaen

Faculty of Medicine, Universitas IndonesiaJakarta, Indonesia
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Silaen, Otto Sahat Martua
  
Sep 20, 2023
Molecular identification of blaTEM and blaCTX-M genes in multidrug-resistant Escherichia coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia's Cover Image
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Published Online: Sep 20, 2023
Page range: 381 - 388
Received: Mar 30, 2023
Accepted: Sep 13, 2023
DOI: https://doi.org/10.2478/jvetres-2023-0052
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© 2023 Agus Widodo et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Escherichia coli is an opportunistic bacteria that can grow easily, produce toxins, and resist antibiotics. The phenomenon of E. coli developing multidrug resistance is currently the subject of extensive research. The objective of this study was to molecularly identify blaTEM and blaCTX-M genes in multidrug-resistant E. coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia.

Material and Methods

One hundred and ten milk samples were collected from 45 dairy cattle farms in Tulungagung, Indonesia. Indole, methyl red, Voges–Proskauer and in citrate tests and triple iron sugar agar tests were used to identify E. coli. Multidrug resistance was determined in isolates through antibiotic sensitivity tests using tetracycline, streptomycin, trimethoprim, chloramphenicol and aztreonam. Extended-spectrum beta lactamase enzyme production was confirmed by double-disc synergy test (DDST). Molecular identification was performed to confirm the blaTEM and blaCTX-M genes.

Results

One hundred and one (91.82%) E. coli strains were isolated from the samples. The antibiotic sensitivity test showed four (3.96%) multidrug-resistant (MDR) and one (0.99%) ESBL-positive E. coli by DDST confirmation. There were three (77.78%) blaTEM genes and one (0.99%) blaCTX-M gene discovered in the MDR E. coli isolates using PCR for molecular identification.

Conclusion

The findings of the blaTEM and blaCTX-M genes encoding ESBL E. coli in dairy cattle milk in Tulungagung, Indonesia is concerning and argues for prompt action to stop the emergence of antibiotic resistance which has an impact on public health.
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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