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Antimicrobial resistance and virulence genes of Streptococcus agalactiae isolated from mastitis milk samples in China

Yankun Zhao

Yankun Zhao

  • Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural SciencesUrumqi, People’s Republic of China
  • Ministry of Agriculture and Rural Affairs Laboratory of Quality and Safety Risk Assessment of Agro-ProductsUrumqi, People’s Republic of China
  • Key Laboratory of Agro-Products Quality and Safety of XinjiangUrumqi, People’s Republic of China
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Zhao, Yankun
, 
Wei Shao

Wei Shao

College of Animal Science, Xinjiang Agriculture UniversityUrumqi, People’s Republic of China
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Shao, Wei
, 
Fulan Wang

Fulan Wang

  • Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural SciencesUrumqi, People’s Republic of China
  • Ministry of Agriculture and Rural Affairs Laboratory of Quality and Safety Risk Assessment of Agro-ProductsUrumqi, People’s Republic of China
  • Key Laboratory of Agro-Products Quality and Safety of XinjiangUrumqi, People’s Republic of China
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Wang, Fulan
, 
Jiaoxiao Ma

Jiaoxiao Ma

  • Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural SciencesUrumqi, People’s Republic of China
  • Ministry of Agriculture and Rural Affairs Laboratory of Quality and Safety Risk Assessment of Agro-ProductsUrumqi, People’s Republic of China
  • Key Laboratory of Agro-Products Quality and Safety of XinjiangUrumqi, People’s Republic of China
  • College of Animal Science, Xinjiang Agriculture UniversityUrumqi, People’s Republic of China
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Ma, Jiaoxiao
, 
He Chen

He Chen

  • Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural SciencesUrumqi, People’s Republic of China
  • Ministry of Agriculture and Rural Affairs Laboratory of Quality and Safety Risk Assessment of Agro-ProductsUrumqi, People’s Republic of China
  • Key Laboratory of Agro-Products Quality and Safety of XinjiangUrumqi, People’s Republic of China
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Chen, He
, 
Shuai Wang

Shuai Wang

  • Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural SciencesUrumqi, People’s Republic of China
  • Ministry of Agriculture and Rural Affairs Laboratory of Quality and Safety Risk Assessment of Agro-ProductsUrumqi, People’s Republic of China
  • Key Laboratory of Agro-Products Quality and Safety of XinjiangUrumqi, People’s Republic of China
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Wang, Shuai
, 
Yating Wu

Yating Wu

  • Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural SciencesUrumqi, People’s Republic of China
  • Ministry of Agriculture and Rural Affairs Laboratory of Quality and Safety Risk Assessment of Agro-ProductsUrumqi, People’s Republic of China
  • Key Laboratory of Agro-Products Quality and Safety of XinjiangUrumqi, People’s Republic of China
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Wu, Yating
, 
Cheng Wang

Cheng Wang

  • Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural SciencesUrumqi, People’s Republic of China
  • Ministry of Agriculture and Rural Affairs Laboratory of Quality and Safety Risk Assessment of Agro-ProductsUrumqi, People’s Republic of China
  • Key Laboratory of Agro-Products Quality and Safety of XinjiangUrumqi, People’s Republic of China
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Wang, Cheng
, 
Nan Zheng

Nan Zheng

Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural SciencesBeijing, People’s Republic of China
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Zheng, Nan
, 
Jiaqi Wang

Jiaqi Wang

Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural SciencesBeijing, People’s Republic of China
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Wang, Jiaqi
 and 
Huimin Liu

Huimin Liu

Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural SciencesBeijing, People’s Republic of China
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Liu, Huimin
  
Dec 16, 2022
Antimicrobial resistance and virulence genes of Streptococcus agalactiae isolated from mastitis milk samples in China's Cover Image
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Published Online: Dec 16, 2022
Page range: 581 - 590
Received: May 07, 2022
Accepted: Dec 05, 2022
DOI: https://doi.org/10.2478/jvetres-2022-0069
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© 2022 Y. Zhao et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Streptococcus agalactiae is an important zoonotic pathogen that affects milk production and quality and poses a threat to public health. Treatment of infections with this bacterium exploits antimicrobials, to which the resistance of S. agalactiae is a growing problem. Addressing the possibility of a correlation between this pathogen’s genetic factors for antimicrobial resistance and virulence, this study attempted to identify the relevant genes.

Material and Methods

Antimicrobial resistance of S. agalactiae isolated from 497 Chinese bovine mastitic milk samples was detected by the broth microdilution method. Eight drug resistance genes and eleven virulence genes were detected using PCR.

Results

Streptococcus agalactiae was 100% susceptible to rifampicin and vancomycin, 93.33% susceptible to sulfisoxazole and sulfamethoxazole, but 100% resistant to ≥3 of the 16 antimicrobial agents, thereby being multidrug resistant, with resistance to oxacillin, tetracycline, erythromycin, clindamycin, and gentamicin being common. The ermB, ermA and lnuA genes were carried by 73.33%, 66.67% and 60.00% of the strains, respectively. The carriage rates of the glnA, clyE, hylB, bibA, iagA, and fbsA virulence genes were greater than 40%, lmb and bac were not observed in any strain, and glnA+hylB+bibA+iagA+fbsA+clyE combined virulence gene patterns were the most commonly detected.

Conclusion

Antimicrobial resistance of S. agalactiae is still a great concern for cattle health in China, and multidrug resistance coupled with the high positive rates of this bacterium’s strains for virulence genes indicates the importance of S. agalactiae surveillance and susceptibility tests.
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