Detection and antimicrobial resistance of Enterobacteriaceae other than Escherichia coli in raccoons from the Madrid region of Spain
, , , , , and | Nov 04, 2022
About this article
Published Online: Nov 04, 2022
Page range: 565 - 569
Received: May 13, 2022
Accepted: Oct 18, 2022
DOI: https://doi.org/10.2478/jvetres-2022-0058
Keywords
© 2022 J.A. Orden et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Identification by sequencing of the 16S ribosomal RNA (16S rRNA) gene and matrix-assisted laser desorption/ionisation–time-of-flight mass spectrometry (MALDI-TOF MS) of 12 Enterobacteriaceae isolates other than E. coli from raccoons in the Madrid region of Spain
| Isolate reference | Sequencing of the 16S rRNA gene | MALDI-TOF MS | |||
|---|---|---|---|---|---|
| Identification | Similarity (%) | First identification (score value) a | Second identification (score value) a | Consistency b | |
| 23147c | 98.8 | D | |||
| 23167b | 98.8 | C | |||
| 23353b | 99.3 | D | |||
| 23375a | 99.5 | D | |||
| 23375c | 99.9 | B | |||
| 23380a | 99.2 | D | |||
| 23380b | 99.1 | D | |||
| 23380c | 99.1 | D | |||
| 23381a | 99.7 | B | |||
| 23381c | 99.0 | B | |||
| 26650a | 99.5 | B | |||
| 26650c | 99.5 | B | |||
Antimicrobial susceptibility of 12 Enterobacteriaceae isolates other than E. coli from raccoons in the Madrid region of Spain
| Isolate reference | Identification | Susceptibility to: | |||||||
|---|---|---|---|---|---|---|---|---|---|
| AMP | AMC | FOX | ST | K | SF | NA | CIP | ||
| 23147c | R | R | R | S | S | I | S | S | |
| 23167b | R | R | R | S | S | I | S | S | |
| 23353b | R | R | R | S | S | I | I | I | |
| 23375a | R | R | R | S | S | S | S | S | |
| 23375c | R | S | S | R | S | S | R | S | |
| 23380a | S | S | S | I | S | I | S | S | |
| 23380b | R | R | S | I | S | S | S | S | |
| 23380c | R | R | S | I | S | S | S | S | |
| 23381a | R | S | S | S | S | I | S | I | |
| 23381c | I | S | S | S | R | R | S | I | |
| 26650a | R | S | S | S | S | I | S | S | |
| 26650c | R | S | S | S | S | I | S | S | |
Interpretive criteria for Enterobacteriaceae using disc diffusion susceptibility testing reported as inhibition zone diameters (mm)
| Antimicrobial agent | Disc (μg) | Susceptible | Intermediate | Resistant |
|---|---|---|---|---|
| Ampicillin | 10 | ≥17 | 14–16 | ≤13 |
| Amoxicillin-clavulanic acid | 30 (20/10) | ≥18 | 14–17 | ≤13 |
| Cefoxitin | 30 | ≥18 | 15–17 | ≤14 |
| Ceftriaxone | 30 | ≥23 | 20–22 | ≤19 |
| Streptomycin | 10 | ≥15 | 12–14 | ≤11 |
| Kanamycin | 30 | ≥18 | 14–17 | ≤13 |
| Amikacin | 30 | ≥17 | 15–16 | ≤14 |
| Gentamicin | 10 | ≥15 | 13–14 | ≤12 |
| Tetracycline | 30 | ≥15 | 12–14 | ≤11 |
| Chloramphenicol | 30 | ≥18 | 13–17 | ≤12 |
| Sulphafurazole | 300 | ≥17 | 13–16 | ≤12 |
| Trimethoprim-sulphamethoxazole | 25 (1.25/23.75) | ≥16 | 11–15 | ≤10 |
| Nalidixic acid | 30 | ≥19 | 14–18 | ≤13 |
| Ciprofloxacin | 5 | ≥26 | 22–25 | ≤21 |