Detection and antimicrobial resistance of Enterobacteriaceae other than Escherichia coli in raccoons from the Madrid region of Spain

Identification by sequencing of the 16S ribosomal RNA (16S rRNA) gene and matrix-assisted laser desorption/ionisation–time-of-flight mass spectrometry (MALDI-TOF MS) of 12 Enterobacteriaceae isolates other than E_ coli from raccoons in the Madrid region of Spain

Isolate reference	Sequencing of the 16S rRNA gene		MALDI-TOF MS
	Identification	Similarity (%)	First identification (score value) a	Second identification (score value) a	Consistency b
23147c	Enterobacter hormaechei subsp. hoffmannii	98.8	Enterobacter cloacae (2.296)	Escherichia coli (2.254)	D
23167b	Citrobacter freundii	98.8	Citrobacter braakii (2.458)	Citrobacter freundii (2.337)	C
23353b	Citrobacter portucalensis	99.3	Citrobacter braakii (2.467)	Citrobacter freundii (2.282)	D
23375a	Citrobacter portucalensis	99.5	Citrobacter freundii (2.289)	Citrobacter braakii (2.261)	D
23375c	Raoultella ornithinolytica	99.9	Raoultella ornithinolytica (2.399)	Raoultella planticola (2.301)	B
23380a	Citrobacter murliniae	99.2	Citrobacter braakii (2.397)	Citrobacter freundii (2.154)	D
23380b	Hafnia paralvei	99.1	Hafnia alvei (2.292)	Dickeya chrysanthemi (1.504)	D
23380c	Hafnia paralvei	99.1	Hafnia alvei (2.292)	Dickeya chrysanthemi (1.504)	D
23381a	Raoultella ornithinolytica	99.7	Raoultella ornithinolytica (2.383)	Raoultella planticola (2.245)	B
23381c	Citrobacter gillenii	99.0	Citrobacter gillenii (2.460)	Citrobacter freundii (2.122)	B
26650a	Citrobacter gillenii	99.5	Citrobacter gillenii (2.408)	Citrobacter freundii (2.140)	B
26650c	Citrobacter gillenii	99.5	Citrobacter gillenii (2.408)	Citrobacter freundii (2.140)	B

Antimicrobial susceptibility of 12 Enterobacteriaceae isolates other than E_ coli from raccoons in the Madrid region of Spain

Isolate reference	Identification	Susceptibility to:
		AMP	AMC	FOX	ST	K	SF	NA	CIP
23147c	Enterobacter hormaechei subsp. hoffmannii	R	R	R	S	S	I	S	S
23167b	Citrobacter freundii	R	R	R	S	S	I	S	S
23353b	Citrobacter portucalensis	R	R	R	S	S	I	I	I
23375a	Citrobacter portucalensis	R	R	R	S	S	S	S	S
23375c	Raoultella ornithinolytica	R	S	S	R	S	S	R	S
23380a	Citrobacter murliniae	S	S	S	I	S	I	S	S
23380b	Hafnia paralvei	R	R	S	I	S	S	S	S
23380c	Hafnia paralvei	R	R	S	I	S	S	S	S
23381a	Raoultella ornithinolytica	R	S	S	S	S	I	S	I
23381c	Citrobacter gillenii	I	S	S	S	R	R	S	I
26650a	Citrobacter gillenii	R	S	S	S	S	I	S	S
26650c	Citrobacter gillenii	R	S	S	S	S	I	S	S

Interpretive criteria for Enterobacteriaceae using disc diffusion susceptibility testing reported as inhibition zone diameters (mm)

Antimicrobial agent	Disc (μg)	Susceptible	Intermediate	Resistant
Ampicillin	10	≥17	14–16	≤13
Amoxicillin-clavulanic acid	30 (20/10)	≥18	14–17	≤13
Cefoxitin	30	≥18	15–17	≤14
Ceftriaxone	30	≥23	20–22	≤19
Streptomycin	10	≥15	12–14	≤11
Kanamycin	30	≥18	14–17	≤13
Amikacin	30	≥17	15–16	≤14
Gentamicin	10	≥15	13–14	≤12
Tetracycline	30	≥15	12–14	≤11
Chloramphenicol	30	≥18	13–17	≤12
Sulphafurazole	300	≥17	13–16	≤12
Trimethoprim-sulphamethoxazole	25 (1.25/23.75)	≥16	11–15	≤10
Nalidixic acid	30	≥19	14–18	≤13
Ciprofloxacin	5	≥26	22–25	≤21