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Influence of direct-fed microbial blend and Ferula elaeochytris on in vitro rumen fermentation pattern and degradability during simulated ruminal acidosis

Ozge Sizmaz

Ozge Sizmaz

  • Department of Animal Nutrition and Nutritional Disease, Faculty of Veterinary Medicine, Ankara UniversityAnkara, Turkey
  • Department of Animal and Poultry Sciences, Virginia Tech UniversityBlacksburg, USA
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Sizmaz, Ozge
, 
Elsayed Mickdam

Elsayed Mickdam

  • Nutrition and Clinical Nutrition Department, South Valley UniversityQena, Egypt
  • Educational and Research Animal Farm, Faculty of Veterinary Medicine, South Valley UniversityQena, Egypt
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Mickdam, Elsayed
 and 
Eyüp Eren Gultepe

Eyüp Eren Gultepe

Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Afyon Kocatepe UniversityAfyonkarahisar, Turkey
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Eren Gultepe, Eyüp
  
Sep 30, 2022
Influence of direct-fed microbial blend and Ferula elaeochytris on in vitro rumen fermentation pattern and degradability during simulated ruminal acidosis's Cover Image
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Published Online: Sep 30, 2022
Page range: 411 - 419
Received: Feb 20, 2022
Accepted: Sep 13, 2022
DOI: https://doi.org/10.2478/jvetres-2022-0052
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© 2022 O. Sizmaz et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

The use of probiotics and phytobiotics has attracted interest because of their protective effect against acidosis. Ferula elaeochytris (FE) is considered a good source of bioactive compounds, mainly monoterpene α-pinene. This study aimed to investigate the effect of a direct-fed microbial blend (Pro) and FE on rumen fermentation parameters in vitro under normal and acidosis conditions.

Material and Methods

An in vitro experiment using the Hohenheimer Futterwerttest (HFT) gas production system was conducted. An acidosis challenge was made to compare the effectiveness of the probiotics blend and FE extract on ruminal pH regulation. To generate different ruminal fermentation parameters, the design of the trial considered the 2 additives (Pro and FE) × 6 incubation times (2, 4, 8, 12, 24 and 48 h) × 2 conditions (acidosis and normal) × 2 incubation runs for each feedstuff (barley, alfalfa and straw).

Results

An acidosis challenge was successfully induced. The Pro and FE additives had no impact on the observed rumen fermentation parameters such as volatile fatty acid concentration or ammonia (P = 0.001). The acidosis condition decreased total in vitro degradability (IVD) by 3.5% and 21.9% for barley and straw, respectively (P < 0.001). The additives had different significant effects on the IVD of nutrients during both normal and acidosis conditions. In alfalfa samples, FE supplementation significantly decreased the IVD of all observed nutrients under the ruminal acidosis condition, although it had no effect during the normal condition.

Conclusion

An acidosis challenge was successfully induced and the effect of additives was varied on fermentation parameters and rumen degradability of different feeds either under normal or acidosis conditions.
Language:
English
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4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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