<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2022-0052_s_005">
<h3>Introduction</h3>
<p>The use of probiotics and phytobiotics has attracted interest because of their protective effect against acidosis. <italic>Ferula elaeochytris</italic> (FE) is considered a good source of bioactive compounds, mainly monoterpene α-pinene. This study aimed to investigate the effect of a direct-fed microbial blend (Pro) and FE on rumen fermentation parameters <italic>in vitro</italic> under normal and acidosis conditions.</p></sec>
<sec id="j_jvetres-2022-0052_s_006">
<h3>Material and Methods</h3>
<p>An <italic>in vitro</italic> experiment using the Hohenheimer Futterwerttest (HFT) gas production system was conducted. An acidosis challenge was made to compare the effectiveness of the probiotics blend and FE extract on ruminal pH regulation. To generate different ruminal fermentation parameters, the design of the trial considered the 2 additives (Pro and FE) × 6 incubation times (2, 4, 8, 12, 24 and 48 h) × 2 conditions (acidosis and normal) × 2 incubation runs for each feedstuff (barley, alfalfa and straw).</p></sec>
<sec id="j_jvetres-2022-0052_s_007">
<h3>Results</h3>
<p>An acidosis challenge was successfully induced. The Pro and FE additives had no impact on the observed rumen fermentation parameters such as volatile fatty acid concentration or ammonia (P = 0.001). The acidosis condition decreased total <italic>in vitro</italic> degradability (IVD) by 3.5% and 21.9% for barley and straw, respectively (P &lt; 0.001). The additives had different significant effects on the IVD of nutrients during both normal and acidosis conditions. In alfalfa samples, FE supplementation significantly decreased the IVD of all observed nutrients under the ruminal acidosis condition, although it had no effect during the normal condition.</p></sec>
<sec id="j_jvetres-2022-0052_s_008">
<h3>Conclusion</h3>
<p>An acidosis challenge was successfully induced and the effect of additives was varied on fermentation parameters and rumen degradability of different feeds either under normal or acidosis conditions.</p></sec>
</abstract>

Introduction
The use of probiotics and phytobiotics has attracted interest because of their protective effect against acidosis. Ferula elaeochytris (FE) is considered a good source of bioactive compounds, mainly monoterpene α-pinene. This study aimed to investigate the effect of a direct-fed microbial blend (Pro) and FE on rumen fermentation parameters in vitro under normal and acidosis conditions.

Material and Methods
An in vitro experiment using the Hohenheimer Futterwerttest (HFT) gas production system was conducted. An acidosis challenge was made to compare the effectiveness of the probiotics blend and FE extract on ruminal pH regulation. To generate different ruminal fermentation parameters, the design of the trial considered the 2 additives (Pro and FE) × 6 incubation times (2, 4, 8, 12, 24 and 48 h) × 2 conditions (acidosis and normal) × 2 incubation runs for each feedstuff (barley, alfalfa and straw).

Results
An acidosis challenge was successfully induced. The Pro and FE additives had no impact on the observed rumen fermentation parameters such as volatile fatty acid concentration or ammonia (P = 0.001). The acidosis condition decreased total in vitro degradability (IVD) by 3.5% and 21.9% for barley and straw, respectively (P < 0.001). The additives had different significant effects on the IVD of nutrients during both normal and acidosis conditions. In alfalfa samples, FE supplementation significantly decreased the IVD of all observed nutrients under the ruminal acidosis condition, although it had no effect during the normal condition.

Conclusion
An acidosis challenge was successfully induced and the effect of additives was varied on fermentation parameters and rumen degradability of different feeds either under normal or acidosis conditions.

Influence of direct-fed microbial blend and <italic>Ferula elaeochytris</italic> on <italic>in vitro</italic> rumen fermentation pattern and degradability during simulated ruminal acidosis

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Influence of direct-fed microbial blend and  on  rumen fermentation pattern and degradability during simulated ruminal acidosis

Introduction
The use of probiotics and phytobiotics has attracted interest because of their protective effect against acidosis. Ferula elaeochytris (FE) is...

Influence of direct-fed microbial blend and Ferula elaeochytris on in vitro rumen fermentation pattern and degradability during simulated ruminal acidosis

Published Online: Sep 30, 2022

Page range: 411 - 419

Received: Feb 20, 2022

Accepted: Sep 13, 2022

DOI: https://doi.org/10.2478/jvetres-2022-0052

Keywords
HFT, phytobiotic, probiotic, α-pinene

© 2022 O. Sizmaz et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Influence of direct-fed microbial blend and Ferula elaeochytris on in vitro rumen fermentation pattern and degradability during simulated ruminal acidosis

Published Online: Sep 30, 2022

Page range: 411 - 419

Received: Feb 20, 2022

Accepted: Sep 13, 2022

DOI: https://doi.org/10.2478/jvetres-2022-0052

KeywordsHFT, phytobiotic, probiotic, α-pinene

© 2022 O. Sizmaz et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
HFT, phytobiotic, probiotic, α-pinene