Determination of anti-phage antibodies in calf sera following application of Escherichia coli and Mannheimia haemolytica-specific bacteriophages

Parameter	FBS			Calves with clinical signs of disease after phage application			Healthy calves after phage application			Healthy calves not treated with phages
Parameter
	IgG	IgM	IgA	IgG	IgM	IgA	IgG	IgM	IgA	IgG	IgM	IgA
Mean level of antibodies against whole M. haemolytica and E. coli bacteriophages	0.007 ± 0.004	0.008 ± 0.006	0.001 ± 0.0009	0.64 ± 0.09^ab	0.192 ± 0.068^ab	0.114 ± 0.048^ab	0.845 ± 0.111^ab	0.239 ± 0.082^ab	0.021 ± 0.007^a	0.056 ± 0.024^a	0.019 ± 0.00^a	0.017 ± 0.01^a
Mean level of antibodies against whole E. coli phages	0.0032 ± 0.001	0.014 ± 0.008	0.001 ± 0.0008	0.74 ± 0.16^ab	0.211 ± 0.052^ab	0.19 ± 0.09^ab	0.71 ± 0.13^ab	0.203 ± 0.2^ab	0.029 ± 0.003^a	0.06 ± 0.009^a	0.096 ± 0.038^a	0.02 ± 0.007^a
Mean level of antibodies against whole M. haemolytica phages	0.005 ± 0.004	0.004 ± 0.0001	0.002 ± 0.0005	0.95 ± 0.08^ab	0.99 ± 0.111^ab	0.23 ± 0.005^ab	0.6 ± 0.1^a	0.33 ± 0.02^ab	0.02 ± 0.002^a	0.72 ± 0.017^a	0.08 ± 0.03^a	0.03 ± 0.001^a
Mean level of antibodies against M. haemolytica and E. coli extracted proteins	0.005 ± 0.003	0.009 ± 0.007	0.001 ± 0.0005	0.71 ± 0.134^ab	0.18 ± 0.34^ab	0.15 ± 0.05^ab	0.53 ± 0.13^ab	0.226 ± 0.13^ab	0.03 ± 0.016	0.06 ± 0.01^a	0.07 ± 0.04^a	0.02 ± 0.0009^a
Mean level of antibodies against E. coli extracted proteins	0.004 ± 0.003	0.002 ± 0.0009	0.001 ± 0.0001	0.83 ± 0.032^ab	0.21 ± 0.004^ab	0.201 ± 0.002^ab	0.68 ± 0.15^ab	0.21 ± 0.004^ab	0.099 ± 0.007^a	0.062 ± 0.001^a	0.042 ± 0.002^a	0.02 ± 0.008^a
Mean level of antibodies against M. haemolytica extracted proteins	0.007 ± 0.004	0.004 ± 0.002	0.001 ± 0.000	0.77 ± 0.13^ab	0.3 ± 0.04^ab	0.15 ± 0.03^ab	0.65 ± 0.21^ab	0.26 ± 0.18 ^ab	0.05 ± 0.02^a	0.071 ± 0.04^a	0.05 ± 0.02^a	0.03 ± 0.01^a

Average values obtained in the phage neutralisation assay

Type of phage solution	Mean K ± SD in untreated calves	Mean K ± SD in treated calves
E. coli phages	0.0019 ± 0.001	7.28 ± 0.54*
M. haemolytica phages	0.0046 ± 0.003	5.57 ± 1.81*
E. coli extracted proteins	0.00156 ± 0.0004	6.74 ± 0.5*
M. haemolytica extracted proteins	0.0013 ± 0.0005	5.053 ± 1.53*

Language:: English

Publication timeframe:: 4 times per year
Journal Subjects:: Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine

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Determination of anti-phage antibodies in calf sera following application of Escherichia coli and Mannheimia haemolytica-specific bacteriophages

Published Online: Aug 12, 2022

Page range: 353 - 360

Received: Jan 17, 2022

Accepted: Jul 21, 2022

DOI: https://doi.org/10.2478/jvetres-2022-0041

Keywords
bacteriophages, ELISA, immunoglobulins

© 2022 R. Urban-Chmiel. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Average absorbance values (±standard deviation) measured in serum samples from calves by ELISA in response to application of bacteriophages specific for E_ coli and M_ haemolytica

Average values obtained in the phage neutralisation assay

Determination of anti-phage antibodies in calf sera following application of Escherichia coli and Mannheimia haemolytica-specific bacteriophages

Renata Urban-Chmiel

Anna Nowaczek

Paulina Chmiel

Jacek Wawrzykowski

Ewelina Pyzik

Agnieszka Marek

Dagmara Stępien-Pyśniak

Mohammed M.M. Alomari

Marta Dec

Published Online: Aug 12, 2022

Page range: 353 - 360

Received: Jan 17, 2022

Accepted: Jul 21, 2022

DOI: https://doi.org/10.2478/jvetres-2022-0041

Keywordsbacteriophages, ELISA, immunoglobulins

© 2022 R. Urban-Chmiel. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Average absorbance values (±standard deviation) measured in serum samples from calves by ELISA in response to application of bacteriophages specific for E_ coli and M_ haemolytica

Average values obtained in the phage neutralisation assay

Keywords
bacteriophages, ELISA, immunoglobulins