Determination of anti-phage antibodies in calf sera following application of  and -specific bacteriophages

Parameter	FBS			Calves with clinical signs of disease after phage application			Healthy calves after phage application			Healthy calves not treated with phages
Parameter
	IgG	IgM	IgA	IgG	IgM	IgA	IgG	IgM	IgA	IgG	IgM	IgA
Mean level of antibodies against whole M. haemolytica and E. coli bacteriophages	0.007 ± 0.004	0.008 ± 0.006	0.001 ± 0.0009	0.64 ± 0.09^ab	0.192 ± 0.068^ab	0.114 ± 0.048^ab	0.845 ± 0.111^ab	0.239 ± 0.082^ab	0.021 ± 0.007^a	0.056 ± 0.024^a	0.019 ± 0.00^a	0.017 ± 0.01^a
Mean level of antibodies against whole E. coli phages	0.0032 ± 0.001	0.014 ± 0.008	0.001 ± 0.0008	0.74 ± 0.16^ab	0.211 ± 0.052^ab	0.19 ± 0.09^ab	0.71 ± 0.13^ab	0.203 ± 0.2^ab	0.029 ± 0.003^a	0.06 ± 0.009^a	0.096 ± 0.038^a	0.02 ± 0.007^a
Mean level of antibodies against whole M. haemolytica phages	0.005 ± 0.004	0.004 ± 0.0001	0.002 ± 0.0005	0.95 ± 0.08^ab	0.99 ± 0.111^ab	0.23 ± 0.005^ab	0.6 ± 0.1^a	0.33 ± 0.02^ab	0.02 ± 0.002^a	0.72 ± 0.017^a	0.08 ± 0.03^a	0.03 ± 0.001^a
Mean level of antibodies against M. haemolytica and E. coli extracted proteins	0.005 ± 0.003	0.009 ± 0.007	0.001 ± 0.0005	0.71 ± 0.134^ab	0.18 ± 0.34^ab	0.15 ± 0.05^ab	0.53 ± 0.13^ab	0.226 ± 0.13^ab	0.03 ± 0.016	0.06 ± 0.01^a	0.07 ± 0.04^a	0.02 ± 0.0009^a
Mean level of antibodies against E. coli extracted proteins	0.004 ± 0.003	0.002 ± 0.0009	0.001 ± 0.0001	0.83 ± 0.032^ab	0.21 ± 0.004^ab	0.201 ± 0.002^ab	0.68 ± 0.15^ab	0.21 ± 0.004^ab	0.099 ± 0.007^a	0.062 ± 0.001^a	0.042 ± 0.002^a	0.02 ± 0.008^a
Mean level of antibodies against M. haemolytica extracted proteins	0.007 ± 0.004	0.004 ± 0.002	0.001 ± 0.000	0.77 ± 0.13^ab	0.3 ± 0.04^ab	0.15 ± 0.03^ab	0.65 ± 0.21^ab	0.26 ± 0.18 ^ab	0.05 ± 0.02^a	0.071 ± 0.04^a	0.05 ± 0.02^a	0.03 ± 0.01^a

Average values obtained in the phage neutralisation assay

Type of phage solution	Mean K ± SD in untreated calves	Mean K ± SD in treated calves
E. coli phages	0.0019 ± 0.001	7.28 ± 0.54*
M. haemolytica phages	0.0046 ± 0.003	5.57 ± 1.81*
E. coli extracted proteins	0.00156 ± 0.0004	6.74 ± 0.5*
M. haemolytica extracted proteins	0.0013 ± 0.0005	5.053 ± 1.53*

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Determination of anti-phage antibodies in calf sera following application of Escherichia coli and Mannheimia haemolytica-specific bacteriophages

Publicado en línea: 12 ago 2022

Páginas: 353 - 360

Recibido: 17 ene 2022

Aceptado: 21 jul 2022

DOI: https://doi.org/10.2478/jvetres-2022-0041

Palabras clave
bacteriophages, ELISA, immunoglobulins

© 2022 R. Urban-Chmiel. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Average absorbance values (±standard deviation) measured in serum samples from calves by ELISA in response to application of bacteriophages specific for E. coli and M. haemolytica

Average values obtained in the phage neutralisation assay

Determination of anti-phage antibodies in calf sera following application of Escherichia coli and Mannheimia haemolytica-specific bacteriophages

Publicado en línea: 12 ago 2022

Páginas: 353 - 360

Recibido: 17 ene 2022

Aceptado: 21 jul 2022

DOI: https://doi.org/10.2478/jvetres-2022-0041

Palabras clavebacteriophages, ELISA, immunoglobulins

© 2022 R. Urban-Chmiel. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Average absorbance values (±standard deviation) measured in serum samples from calves by ELISA in response to application of bacteriophages specific for E. coli and M. haemolytica

Average values obtained in the phage neutralisation assay

Palabras clave
bacteriophages, ELISA, immunoglobulins