Laboratory experience with the microscopic method for the detection of insects in poultry feeds
Published Online: Jul 30, 2022
Page range: 403 - 409
Received: Feb 08, 2022
Accepted: Jul 13, 2022
DOI: https://doi.org/10.2478/jvetres-2022-0040
Keywords
© 2022 A.Weiner, K. Kwiatek. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
The use of insects and their processed animal proteins (PAPs) for animal nutrition creates the need for research into methods useful for routine surveillance for their presence. The aim of this study was to evaluate a modified microscopic method for the detection of particles of insects in poultry feed.
Material and Methods
A total of 90 samples including PAP of insects (
Results
Characteristic features of insects including cuticulae, muscles, bristles and tracheoles were determined in the microscopic images obtained. In all spiked samples, characteristic fragments of insects were detected. The fragments of muscle and tracheoles only indicated the presence of material from members of the insect class but could not facilitate identification of organisms to species level.
Conclusion
The results obtained with this double sedimentation protocol for the isolation of insect PAP from feed for poultry have shown that the method can be used in routine analysis.