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Mutations of p53 gene in canine sweat gland carcinomas probably associated with UV radiation

Agnieszka Jasik
Agnieszka Jasik
, 
Anna Kycko
Anna Kycko
, 
Monika Olech
Monika Olech
, 
Krzysztof Wyrostek
Krzysztof Wyrostek
, 
Anna Śmiech
Anna Śmiech
, 
Wojciech Łopuszyński
Wojciech Łopuszyński
, 
Iwona Otrocka-Domagała
Iwona Otrocka-Domagała
, 
Mateusz Mikiewicz
Mateusz Mikiewicz
 and 
Izabella Dolka
Izabella Dolka
   | Dec 20, 2021
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Article Category: Review article
Published Online: Dec 20, 2021
Page range: 519 - 526
Received: Aug 13, 2021
Accepted: Dec 09, 2021
DOI: https://doi.org/10.2478/jvetres-2021-0070
Keywords
© 2021 A. Jasik et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Example images of the laser capture microdissection of tumour cells acquired using Arcturus XT operating software (10× objective). A – Haematoxylin and eosin stained section, covered with xylene for visualisation, showing cystic/papillary type apocrine sweat gland carcinoma before microdissection, with the area containing tumour cells marked with a red line. B – The same section with marked tumour cells and laser infrared spots (IR spots). C – The same section after xylene evaporation and the microdissection of the marked cells. D – Tumour cells transferred to the macro cap after laser capture microdissection
Example images of the laser capture microdissection of tumour cells acquired using Arcturus XT operating software (10× objective). A – Haematoxylin and eosin stained section, covered with xylene for visualisation, showing cystic/papillary type apocrine sweat gland carcinoma before microdissection, with the area containing tumour cells marked with a red line. B – The same section with marked tumour cells and laser infrared spots (IR spots). C – The same section after xylene evaporation and the microdissection of the marked cells. D – Tumour cells transferred to the macro cap after laser capture microdissection

Fig. 2

Alignment of the amino acid sequence of exon 4 of the p53 protein of the canine control (K1) sample and tested samples of apocrine sweat gland carcinomas with canine (U62133, AB020761) and human (K03199) amino acid reference sequences of the p53 protein
Alignment of the amino acid sequence of exon 4 of the p53 protein of the canine control (K1) sample and tested samples of apocrine sweat gland carcinomas with canine (U62133, AB020761) and human (K03199) amino acid reference sequences of the p53 protein

Fig. 3

Alignment of the amino acid sequence of exon 5 of the p53 protein of the canine control (K1) sample and tested samples of apocrine sweat gland carcinomas with canine (U62133, AB020761) and human (K03199) amino acid references sequence of the p53 protein
Alignment of the amino acid sequence of exon 5 of the p53 protein of the canine control (K1) sample and tested samples of apocrine sweat gland carcinomas with canine (U62133, AB020761) and human (K03199) amino acid references sequence of the p53 protein

Fig. 4

Alignment of the amino acid sequence of exon 6 of the p53 protein of the canine control (K1) sample and tested samples of apocrine sweat gland carcinoma with canine (U62133, AB020761) and human (K03199) amino acid reference sequences of the p53 protein
Alignment of the amino acid sequence of exon 6 of the p53 protein of the canine control (K1) sample and tested samples of apocrine sweat gland carcinoma with canine (U62133, AB020761) and human (K03199) amino acid reference sequences of the p53 protein

Fig. 5

Alignment of the amino acid sequence of exon 8 of p53 of the canine control (K1) sample and tested samples of apocrine sweat gland carcinomas with canine (U62133, AB020761) and human (K03199) amino acid reference sequences of the p53 protein
Alignment of the amino acid sequence of exon 8 of p53 of the canine control (K1) sample and tested samples of apocrine sweat gland carcinomas with canine (U62133, AB020761) and human (K03199) amino acid reference sequences of the p53 protein

Mutations in the p53 gene in canine ASGC

Sample no. Histology* Breed Age (years) Body location Exon Codon † Mutation (amino acid)
180 Papillary ASGC — 5 Chest (rib area) 5 157 gTc → gCc (Val → Ala)
188 Papillary ASGC German shepherd 6 Forelimb (arm area) 8 264 aAc →a-c reading frame shift
190 Solid ASGC Mixed breed 8 Neck (side neck area) 5 178 caC → caT (His = His)
191 Ductal ASGC Mixed breed 10 Chest (scapular area) 5 149, 150 tcCCca → tcTTca (Ser = Ser; Pro > Ser)
193 Solid ASGC Dachshund 9 Forelimb (axillary area) 5 157 gTc → gCc (Val → Ala)
gcT → gcC
63 (Ala = Ala)
194 Tubular ASGC Giant schnauzer — Finger 4 6 123 aCt → aGt (Thr → Ser)
214 aCt → aGt
(Thr → Ser)
201 Ductal ASGC Mixed breed 12 Head (auricle area) 5 157 gTc → gCc (Val → Ala)
212 Papillary ASGC Mixed breed 11 — 4 90 tCc → tTc (Ser → Phe)
217 Ductal ASGC Mixed breed 5 — 4 90 tCc → tTc
(Ser → Phe)
219 Cystic, ASGC papillary Mixed breed 5 Pelvis (gluteal area) 4 90 tCc → tTc (Ser → Phe)
220 Solid ASGC — 11 Pelvis (anal area) 4 90 tCc → tTc (Ser → Phe)
221 Solid ASGC French bulldog 11 — 4 90 tCc → tTc (Ser → Phe)
222 Carcinosarcoma ASG Miniature schnauzer 8 Chest (rib area) 4 90 tCc → tTc (Ser → Phe)
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