<abstract xmlns="http://www.w3.org/1999/xhtml">
<sec id="j_jvetres-2021-0070_s_005">
<h3>Introduction</h3>
<p>Apocrine sweat gland carcinomas (ASGCs) are rare malignant skin tumours in dogs and humans. The literature published so far focuses mostly on the clinico-epidemiological aspect of these tumours, but little is known about their pathogenesis. In this study we aimed to determine whether the <italic>p53</italic> gene is involved in the carcinogenesis of the apocrine sweat gland in dogs and whether ultraviolet radiation (UV) is related to it.</p></sec>
<sec id="j_jvetres-2021-0070_s_006">
<h3>Material and Methods</h3>
<p>Forty canine ASGCs were submitted to laser capture microdissection to isolate neoplastic cells, from which DNA was subsequently extracted. PCR amplification and sequencing of <italic>p53</italic> exons 2–8 was then performed, followed by computer analysis of the obtained sequences.</p></sec>
<sec id="j_jvetres-2021-0070_s_007">
<h3>Results</h3>
<p>Sixteen mutations within the <italic>p53</italic> gene were found in 13 tumours. The mutations involved C → T, T → C, G → A, and CC → TT transitions, C → G transversion and adenine deletion, which are gene alteration types known to be related to UV radiation in the process of skin carcinogenesis in humans. Six of the thirteen tumour cases displayed the C → T transitions in the same location in exon 4 and three of the thirteen cases displayed T → C in the same location in exon 5.</p></sec>
<sec id="j_jvetres-2021-0070_s_008">
<h3>Conclusion</h3>
<p>The results of the present study indicate both the participation of the <italic>p53</italic> gene and the influence of UV radiation in the formation of ASGCs in dogs.</p></sec>
</abstract>

Introduction
Apocrine sweat gland carcinomas (ASGCs) are rare malignant skin tumours in dogs and humans. The literature published so far focuses mostly on the clinico-epidemiological aspect of these tumours, but little is known about their pathogenesis. In this study we aimed to determine whether the p53 gene is involved in the carcinogenesis of the apocrine sweat gland in dogs and whether ultraviolet radiation (UV) is related to it.

Material and Methods
Forty canine ASGCs were submitted to laser capture microdissection to isolate neoplastic cells, from which DNA was subsequently extracted. PCR amplification and sequencing of p53 exons 2–8 was then performed, followed by computer analysis of the obtained sequences.

Results
Sixteen mutations within the p53 gene were found in 13 tumours. The mutations involved C → T, T → C, G → A, and CC → TT transitions, C → G transversion and adenine deletion, which are gene alteration types known to be related to UV radiation in the process of skin carcinogenesis in humans. Six of the thirteen tumour cases displayed the C → T transitions in the same location in exon 4 and three of the thirteen cases displayed T → C in the same location in exon 5.

Conclusion
The results of the present study indicate both the participation of the p53 gene and the influence of UV radiation in the formation of ASGCs in dogs.

Mutations of <italic>p53</italic> gene in canine sweat gland carcinomas probably associated with UV radiation

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mutations of  gene in canine sweat gland carcinomas probably associated with UV radiation

Introduction
Apocrine sweat gland carcinomas (ASGCs) are rare malignant skin tumours in dogs and humans. The literature published so far focuses mostly on the...

Mutations of p53 gene in canine sweat gland carcinomas probably associated with UV radiation

Article Category: Review article

Published Online: Dec 20, 2021

Page range: 519 - 526

Received: Aug 13, 2021

Accepted: Dec 09, 2021

DOI: https://doi.org/10.2478/jvetres-2021-0070

Keywords
dog, apocrine sweat gland carcinoma, gene, mutations, UVR

© 2021 A. Jasik et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mutations of p53 gene in canine sweat gland carcinomas probably associated with UV radiation

Article Category: Review article

Published Online: Dec 20, 2021

Page range: 519 - 526

Received: Aug 13, 2021

Accepted: Dec 09, 2021

DOI: https://doi.org/10.2478/jvetres-2021-0070

Keywordsdog, apocrine sweat gland carcinoma, gene, mutations, UVR

© 2021 A. Jasik et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
dog, apocrine sweat gland carcinoma, gene, mutations, UVR