<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2020-0041_s_005_w2aab3b7c43b1b6b1aab1c15b1Aa"><h3>Introduction</h3><p>African swine fever (ASF) was officially reported in Vietnam in February 2019 and spread across the whole country, affecting all 63 provinces and cities.</p></sec><sec id="j_jvetres-2020-0041_s_006_w2aab3b7c43b1b6b1aab1c15b2Aa"><h3>Material and Methods</h3><p>In this study, ASF virus (ASFV) VN/Pig/HaNam/2019 (VN/Pig/HN/19) strain was isolated in primary porcine alveolar macrophage (PAM) cells from a sample originating from an outbreak farm in Vietnam’s Red River Delta region. The isolate was characterised using the haemadsorption (HAD) test, real-time PCR, and sequencing. The activity of antimicrobial feed products was evaluated <italic>via</italic> a contaminated ASFV feed assay.</p></sec><sec id="j_jvetres-2020-0041_s_007_w2aab3b7c43b1b6b1aab1c15b3Aa"><h3>Results</h3><p>Phylogenetic analysis of the viral p72 and EP402R genes placed VN/Pig/HN/19 in genotype II and serogroup 8 and related it closely to Eastern European and Chinese strains. Infectious titres of the virus propagated in primary PAMs were 10<sup>6</sup> HAD<sub>50</sub>/ml. Our study reports the activity against ASFV VN/Pig/HN/19 strain of antimicrobial Sal CURB RM E Liquid, F2 Dry and K2 Liquid. Our feed assay findings suggest that the antimicrobial RM E Liquid has a strong effect against ASFV replication. These results suggest that among the Sal CURB products, the antimicrobial RM E Liquid may have the most potential as a mitigant feed additive for ASFV infection. Therefore, further studies on the use of antimicrobial Sal CURB RM E Liquid <italic>in vivo</italic> are required.</p></sec><sec id="j_jvetres-2020-0041_s_008_w2aab3b7c43b1b6b1aab1c15b4Aa"><h3>Conclusions</h3><p>Our study demonstrates the threat of ASFV and emphasises the need to control and eradicate it in Vietnam by multiple measures.</p></sec></abstract>

IntroductionAfrican swine fever (ASF) was officially reported in Vietnam in February 2019 and spread across the whole country, affecting all 63 provinces and cities.Material and MethodsIn this study, ASF virus (ASFV) VN/Pig/HaNam/2019 (VN/Pig/HN/19) strain was isolated in primary porcine alveolar macrophage (PAM) cells from a sample originating from an outbreak farm in Vietnam’s Red River Delta region. The isolate was characterised using the haemadsorption (HAD) test, real-time PCR, and sequencing. The activity of antimicrobial feed products was evaluated via a contaminated ASFV feed assay.ResultsPhylogenetic analysis of the viral p72 and EP402R genes placed VN/Pig/HN/19 in genotype II and serogroup 8 and related it closely to Eastern European and Chinese strains. Infectious titres of the virus propagated in primary PAMs were 106 HAD50/ml. Our study reports the activity against ASFV VN/Pig/HN/19 strain of antimicrobial Sal CURB RM E Liquid, F2 Dry and K2 Liquid. Our feed assay findings suggest that the antimicrobial RM E Liquid has a strong effect against ASFV replication. These results suggest that among the Sal CURB products, the antimicrobial RM E Liquid may have the most potential as a mitigant feed additive for ASFV infection. Therefore, further studies on the use of antimicrobial Sal CURB RM E Liquid in vivo are required.ConclusionsOur study demonstrates the threat of ASFV and emphasises the need to control and eradicate it in Vietnam by multiple measures.

Genetic characterisation of African swine fever virus in outbreaks in Ha Nam province, Red River Delta Region of Vietnam, and activity of antimicrobial products against virus infection in contaminated feed

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

IntroductionAfrican swine fever (ASF) was officially reported in Vietnam in February 2019 and spread across the whole country, affecting all 63 provinces and...

Genetic characterisation of African swine fever virus in outbreaks in Ha Nam province, Red River Delta Region of Vietnam, and activity of antimicrobial products against virus infection in contaminated feed

Published Online: Jun 16, 2020

Page range: 207 - 213

Received: Nov 11, 2019

Accepted: Jun 01, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0041

Keywords
pig, African swine fever virus, virus isolation, Sal CURB, Vietnam

© 2020 H.T.T. Tran et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genetic characterisation of African swine fever virus in outbreaks in Ha Nam province, Red River Delta Region of Vietnam, and activity of antimicrobial products against virus infection in contaminated feed

Published Online: Jun 16, 2020

Page range: 207 - 213

Received: Nov 11, 2019

Accepted: Jun 01, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0041

Keywordspig, African swine fever virus, virus isolation, Sal CURB, Vietnam

© 2020 H.T.T. Tran et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
pig, African swine fever virus, virus isolation, Sal CURB, Vietnam